IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心
Different responses in one-year-old and three-year-old grass carp reveal the mechanism of age restriction of GCRV infection
Chen, Geng1,2; Xiong, Lv1,2; Wang, Yumeng1,3; He, Libo1; Huang, Rong1; Liao, Lanjie1; Zhu, Zuoyan1; Wang, Yaping1
2019-03-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume86Issue:1Pages:702-712
AbstractGrass carp is an important fish species in Chinese aquaculture, and can be afflicted by a hemorrhagic disease caused by the grass carp reovirus (GCRV). Interestingly, the affects of GCRV infection of grass carp are age-restricted, meaning that one-year-old grass carp can be infected and can suffer hemorrhagic disease, but three-year-old carp are not so afflicted. In this study, we investigated the mechanism responsible for this age-restricted pathology. We evaluated the relative copy number of GCRV RNA, the expression levels of proteins in blood, and changes in DNA methylation in carp from the two age groups after infection with GCRV. After GCRV infection, the relative copy number of GCRV RNA in three-year-old grass carp was significantly lower than in one-year-old carp. The differences in circulating protein levels mainly occurred in concentrated in complement and coagulation proteins, and the expression levels of these proteins were significantly higher in three-year-old grass carp than in one-year-old carp. Moreover, the expression levels of DNA methylation-related genes in the liver and spleen of one-year-old grass carp were significantly higher than those of three-year-old carp. These results suggested that as age of grass carp increases, faster and more efficient response of the immune system after viral infection, especially the complement system, and differences in DNA methylation may be important factors that affect the age restriction observed in GCRV infection. Our study provides new insights into the mechanisms underlying age restriction of GCRV infection.
SubtypeArticle
KeywordGrass Carp Grass Carp Reovirus Age Restriction Complement Dna Methylation
DOI10.1016/j.fsi.2018.11.074
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationNational Natural Science Foundation of China(31721005 ; National Natural Science Foundation of China(31721005 ; 31702332) ; 31702332) ; National Natural Science Foundation of China(31721005 ; National Natural Science Foundation of China(31721005 ; 31702332) ; 31702332)
Language英语
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS KeywordMESSENGER-RNA EXPRESSION ; NF-KAPPA-B ; CTENOPHARYNGODON-IDELLUS ; RAINBOW-TROUT ; SNP DETECTION ; I GENE ; VIRUS ; REOVIRUS ; ASSOCIATION ; METHYLATION
WOS IDWOS:000459528400079
Funding OrganizationNational Natural Science Foundation of China(31721005 ; National Natural Science Foundation of China(31721005 ; 31702332) ; 31702332) ; National Natural Science Foundation of China(31721005 ; National Natural Science Foundation of China(31721005 ; 31702332) ; 31702332)
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/72461
Collection鱼类生物学及渔业生物技术研究中心
Corresponding AuthorWang, Yaping
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Wuhan Univ, Coll Life Sci, Wuhan, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Chen, Geng,Xiong, Lv,Wang, Yumeng,et al. Different responses in one-year-old and three-year-old grass carp reveal the mechanism of age restriction of GCRV infection[J]. FISH & SHELLFISH IMMUNOLOGY,2019,86(1):702-712.
APA Chen, Geng.,Xiong, Lv.,Wang, Yumeng.,He, Libo.,Huang, Rong.,...&Wang, Yaping.(2019).Different responses in one-year-old and three-year-old grass carp reveal the mechanism of age restriction of GCRV infection.FISH & SHELLFISH IMMUNOLOGY,86(1),702-712.
MLA Chen, Geng,et al."Different responses in one-year-old and three-year-old grass carp reveal the mechanism of age restriction of GCRV infection".FISH & SHELLFISH IMMUNOLOGY 86.1(2019):702-712.
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