IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心
Cloning of six serpin genes and their responses to GCRV infection in grass carp (Ctenopharyngodon idella)
Chen, Liangming1,2; Huang, Rong1; Zhu, Denghui1,2; Wang, Yumeng3; Mehjabin, Rumana1,2; Li, Yongming1; Liao, Lanjie1; He, Libo1; Zhu, Zuoyan1; Wang, Yaping1
2019-03-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume86Issue:1Pages:93-100
AbstractGrass carp, an economically important aquaculture fish, is very sensitive to Grass Carp Reovirus (GCRV). Haemorrhagic disease caused by GCRV infection can cause large-scale death of first-year grass carp, thereby severely restricting the intensive culture. Serpins (serine protease inhibitors) belong to the protease inhibitor gene family and are involved in numerous physiological and pathological processes, particularly coagulation and anticoagulation. Reports on grass carp serpins are scarce. Thus, we cloned six grass carp serpin genes (serpinb1, serpinc1, serpind1, serpinf1, serpinf2b and serping1) in this study. Molecular evolution showed that serpins between grass carp and zebrafish or carp are the closest relatives. SERPIN domains in these 6 serpins and reactive centre loop (RCL) along with their cleavage sites of 5 serpins (serpinb1, serpinc1, serpind1, serpinf2b and serping1) were predicted. Real-time quantitative PCR (RT-qPCR) showed that these serpins displayed tissue significance. Among them, serpinc1, serpind1, serpinf2b and serping1 had the highest expression levels in the liver. After GCRV infection, RT-qPCR showed that the liver-enriched serpins were significantly changed. Key procoagulant factor genes (kng-1, f2, f3a, f3b and f7) and anticoagulant genes (gm, pig, thbd, proc and pros) also showed significant changes on the mRNA level. Comprehensive comparative analysis showed that the up-regulated expression of key clotting factor genes was more prominent than that of main anti-coagulation factor genes. Thus, the function of coagulation may be more dominant in grass carp during the GCRV infection, which may cause overproduction of thrombi. The serpins were involved in GCRV infection and liver-enriched serpins participate in the interaction between coagulation and anticoagulation. This study provided new insights into further research on the biological functions of grass carp serpins and clarifying the molecular mechanism of GCRV affecting the homeostasis of grass carp blood environment.
SubtypeArticle
KeywordGrass Carp Grass Carp Reovirus (Gcrv) Serpins (Serine Protease Inhibitors) Coagulation And antiCoagulation
DOI10.1016/j.fsi.2018.11.008
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationNational Natural Science Foundation of China(31572614) ; National Natural Science Foundation of China(31572614) ; Strategic Priority Research Program of Chinese Academy of Sciences(XDA08030203) ; Strategic Priority Research Program of Chinese Academy of Sciences(XDA08030203) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2016FBZ02) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2016FBZ02) ; National Natural Science Foundation of China(31572614) ; National Natural Science Foundation of China(31572614) ; Strategic Priority Research Program of Chinese Academy of Sciences(XDA08030203) ; Strategic Priority Research Program of Chinese Academy of Sciences(XDA08030203) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2016FBZ02) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2016FBZ02)
Language英语
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS KeywordBLOOD-COAGULATION ; MOLECULAR-CLONING ; INHIBITORS ; HEMOSTASIS ; INITIATION ; CASCADE
WOS IDWOS:000459528400011
Funding OrganizationNational Natural Science Foundation of China(31572614) ; National Natural Science Foundation of China(31572614) ; Strategic Priority Research Program of Chinese Academy of Sciences(XDA08030203) ; Strategic Priority Research Program of Chinese Academy of Sciences(XDA08030203) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2016FBZ02) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2016FBZ02) ; National Natural Science Foundation of China(31572614) ; National Natural Science Foundation of China(31572614) ; Strategic Priority Research Program of Chinese Academy of Sciences(XDA08030203) ; Strategic Priority Research Program of Chinese Academy of Sciences(XDA08030203) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2016FBZ02) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2016FBZ02)
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/72460
Collection鱼类生物学及渔业生物技术研究中心
Corresponding AuthorHuang, Rong; Wang, Yaping
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Wuhan Univ, Wuhan 430072, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Chen, Liangming,Huang, Rong,Zhu, Denghui,et al. Cloning of six serpin genes and their responses to GCRV infection in grass carp (Ctenopharyngodon idella)[J]. FISH & SHELLFISH IMMUNOLOGY,2019,86(1):93-100.
APA Chen, Liangming.,Huang, Rong.,Zhu, Denghui.,Wang, Yumeng.,Mehjabin, Rumana.,...&Wang, Yaping.(2019).Cloning of six serpin genes and their responses to GCRV infection in grass carp (Ctenopharyngodon idella).FISH & SHELLFISH IMMUNOLOGY,86(1),93-100.
MLA Chen, Liangming,et al."Cloning of six serpin genes and their responses to GCRV infection in grass carp (Ctenopharyngodon idella)".FISH & SHELLFISH IMMUNOLOGY 86.1(2019):93-100.
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