IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心
Deep sequencing of small RNAs from 11 tissues of grass carp Ctenopharyngodon idella and discovery of sex-related microRNAs
Chen, Liangming1,2; Huang, Rong1; Zhu, Denghui1,2; Yang, Cheng1,2; He, Libo1; Li, Yongming1; Liao, Lanjie1; Zhu, Zuoyan1; Wang, Yaping1
2019
Source PublicationJOURNAL OF FISH BIOLOGY
ISSN0022-1112
Volume94Issue:1Pages:132-141
AbstractThis research identified 169 known microRNAs (miRNAs), 380 novel miRNAs, and 30,538 targets in 11 tissues (blood, brain, derma, gill, heart, intestine, kidney, liver, muscle, pronephros, and spleen) from grass carp Ctenopharyngodon idella with high-throughput sequencing (HTS). Transcripts per million (TPM) expression analysis detected 41 brain-enriched miRNAs (accounting for 61.19% of all tissue-enriched miRNAs). Real-time quantitative PCR (RT-qPCR) confirmed that 21 of 24 randomly selected tissue-enriched miRNAs from the TPM analysis were indeed tissue-enriched (P < 0.05), suggesting the HTS and TPM analyses were reliable. Nine of the 41 brain-enriched miRNAs are complementary to members of the double-sex and mab-3 related transcription factor family (dmrt) involved in sex differentiation. RT-qPCR revealed that cid-miR-138 was more highly expressed in testis than in ovary (P < 0.01), while the reverse was true for target gene dmrt4a (P < 0.01). This opposite expression pattern suggested the direct participation of cid-miR-138-dmrt4a in neuroendocrine mechanisms related to brain-pituitary networks during sex development. The discovery of miRNAs from 11 C. idella tissues expands the available fish miRNA database, and enhances our understanding of the role of sex-related miRNAs in tissue differentiation and maintenance of specific tissue functions in fishes.
SubtypeArticle
KeywordDeep Sequencing Dmrt Grass Carp Microrna Sex Differentiation
DOI10.1111/jfb.13875
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationNational Natural Science Foundation of China(31572614) ; Strategic Priority Research Program of Chinese Academy of Sciences(XDA08030203) ; State of Key Laboratory of Freshwater Ecology and Biotechnology(2016FBZ02) ; National Natural Science Foundation of China(31572614) ; Strategic Priority Research Program of Chinese Academy of Sciences(XDA08030203) ; State of Key Laboratory of Freshwater Ecology and Biotechnology(2016FBZ02)
Language英语
WOS Research AreaFisheries ; Marine & Freshwater Biology
WOS SubjectFisheries ; Marine & Freshwater Biology
WOS KeywordEXPRESSION ; GENES ; EVOLUTION ; IDENTIFICATION ; MECHANISMS ; INSIGHTS ; GENOME ; TARGET ; FISH ; KEGG
WOS IDWOS:000457463200015
Funding OrganizationNational Natural Science Foundation of China(31572614) ; Strategic Priority Research Program of Chinese Academy of Sciences(XDA08030203) ; State of Key Laboratory of Freshwater Ecology and Biotechnology(2016FBZ02) ; National Natural Science Foundation of China(31572614) ; Strategic Priority Research Program of Chinese Academy of Sciences(XDA08030203) ; State of Key Laboratory of Freshwater Ecology and Biotechnology(2016FBZ02)
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/72379
Collection鱼类生物学及渔业生物技术研究中心
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Chen, Liangming,Huang, Rong,Zhu, Denghui,et al. Deep sequencing of small RNAs from 11 tissues of grass carp Ctenopharyngodon idella and discovery of sex-related microRNAs[J]. JOURNAL OF FISH BIOLOGY,2019,94(1):132-141.
APA Chen, Liangming.,Huang, Rong.,Zhu, Denghui.,Yang, Cheng.,He, Libo.,...&Wang, Yaping.(2019).Deep sequencing of small RNAs from 11 tissues of grass carp Ctenopharyngodon idella and discovery of sex-related microRNAs.JOURNAL OF FISH BIOLOGY,94(1),132-141.
MLA Chen, Liangming,et al."Deep sequencing of small RNAs from 11 tissues of grass carp Ctenopharyngodon idella and discovery of sex-related microRNAs".JOURNAL OF FISH BIOLOGY 94.1(2019):132-141.
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