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Molecular characterization of eukaryotic algal communities in the tropical phyllosphere based on real-time sequencing of the 18S rDNA gene
Zhu, Huan1; Li, Shuyin1; Hu, Zhengyu2; Liu, Guoxiang1
2018-12-18
Source PublicationBMC PLANT BIOLOGY
ISSN1471-2229
Volume18Issue:365Pages:1-14
AbstractBackgroudFoliicolous algae are a common occurrence in tropical forests. They are referable to a few simple morphotypes (unicellular, sarcinoid-like or filamentous), which makes their morphology of limited usefulness for taxonomic studies and species diversity assessments. The relationship between algal community and their host phyllosphere was not clear. In order to obtain a more accurate assessment, we used single molecule real-time sequencing of the 18S rDNA gene to characterize the eukaryotic algal community in an area of South-western China.ResultWe annotated 2922 OTUs belonging to five classes, Ulvophyceae, Trebouxiophyceae, Chlorophyceae, Dinophyceae and Eustigmatophyceae. Novel clades formed by large numbers sequences of green algae were detected in the order Trentepohliales (Ulvophyceae) and the Watanabea clade (Trebouxiophyceae), suggesting that these foliicolous communities may be substantially more diverse than so far appreciated and require further research. Species in Trentepohliales, Watanabea clade and Apatococcus clade were detected as the core members in the phyllosphere community studied. Communities from different host trees and sampling sites were not significantly different in terms of OTUs composition. However, the communities of Musa and Ravenala differed from other host plants significantly at the genus level, since they were dominated by Trebouxiophycean epiphytes.ConclusionThe cryptic diversity of eukaryotic algae especially Chlorophytes in tropical phyllosphere is very high. The community structure at species-level has no significant relationship either with host phyllosphere or locations. The core algal community in tropical phyllopshere is consisted of members from Trentepohliales, Watanabea clade and Apatococcus clade. Our study provided a large amount of novel 18S rDNA sequences that will be useful to unravel the cryptic diversity of phyllosphere eukaryotic algae and for comparisons with similar future studies on this type of communities.
SubtypeArticle
KeywordAerial Algae Community Structure Diversity Phyllosphere Phylogeny Smrt Sequencing Tropical Forest
DOI10.1186/s12870-018-1588-7
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationNational Natural Science Foundation of China(31600168 ; Chinese Academy of Sciences Key deployment project(ZDRWZS-2017-221) ; 31870189) ; National Natural Science Foundation of China(31600168 ; Chinese Academy of Sciences Key deployment project(ZDRWZS-2017-221) ; 31870189)
Language英语
WOS Research AreaPlant Sciences
WOS SubjectPlant Sciences
WOS KeywordCOCCOID GREEN-ALGAE ; SP-NOV TREBOUXIOPHYCEAE ; TRENTEPOHLIALES ULVOPHYCEAE ; CORTICOLOUS MICROALGAE ; SUBAERIAL ALGAE ; CHLOROPHYTA ; DIVERSITY ; TREE ; SYSTEMATICS ; MORPHOLOGY
WOS IDWOS:000453706200002
Funding OrganizationNational Natural Science Foundation of China(31600168 ; Chinese Academy of Sciences Key deployment project(ZDRWZS-2017-221) ; 31870189) ; National Natural Science Foundation of China(31600168 ; Chinese Academy of Sciences Key deployment project(ZDRWZS-2017-221) ; 31870189)
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/67779
Collection藻类生物学及应用研究中心
Affiliation1.Chinese Acad Sci, Key Lab Algal Biol, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Zhu, Huan,Li, Shuyin,Hu, Zhengyu,et al. Molecular characterization of eukaryotic algal communities in the tropical phyllosphere based on real-time sequencing of the 18S rDNA gene[J]. BMC PLANT BIOLOGY,2018,18(365):1-14.
APA Zhu, Huan,Li, Shuyin,Hu, Zhengyu,&Liu, Guoxiang.(2018).Molecular characterization of eukaryotic algal communities in the tropical phyllosphere based on real-time sequencing of the 18S rDNA gene.BMC PLANT BIOLOGY,18(365),1-14.
MLA Zhu, Huan,et al."Molecular characterization of eukaryotic algal communities in the tropical phyllosphere based on real-time sequencing of the 18S rDNA gene".BMC PLANT BIOLOGY 18.365(2018):1-14.
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