IHB OpenIR  > 藻类生物技术和生物能源研发中心
Comparative transcriptome analyses of oleaginous Botryococcus braunii race A reveal significant differences in gene expression upon cobalt enrichment
Cheng, Pengfei1; Zhou, Chengxu1; Wang, Yan1; Xu, Zhihui1; Xu, Jilin1; Zhou, Dongqing2,3; Zhang, Yinghui2,3; Wu, Haizhen2,3; Zhang, Xuezhi4; Liu, Tianzhong5; Tang, Ming6; Yang, Qiyong6; Yan, Xiaojun7; Fan, Jianhua2,3
2018-12-18
Source PublicationBIOTECHNOLOGY FOR BIOFUELS
ISSN1754-6834
Volume11Issue:333Pages:1
AbstractBackgroundBotryococcus braunii is known for its high hydrocarbon content, thus making it a strong candidate feedstock for biofuel production. Previous study has revealed that a high cobalt concentration can promote hydrocarbon synthesis and it has little effect on growth of B. braunii cells. However, mechanisms beyond the cobalt enrichment remain unknown. This study seeks to explore the physiological and transcriptional response and the metabolic pathways involved in cobalt-induced hydrocarbon synthesis in algae cells.ResultsGrowth curves were similar at either normal or high cobalt concentration (4.5mg/L), suggesting the absence of obvious deleterious effects on growth introduced by cobalt. Photosynthesis indicators (decline in Fv/Fm ratio and chlorophyll content) and reactive oxygen species parameters revealed an increase in physiological stress in the high cobalt concentration. Moreover, cobalt enrichment treatment resulted in higher crude hydrocarbon content (51.3% on day 8) compared with the control (43.4% on day 8) throughout the experiment (with 18.2% improvement finally). Through the de novo assembly and functional annotation of the B. braunii race A SAG 807-1 transcriptome, we retrieved 196,276 non-redundant unigenes with an average length of 1086bp. Of the assembled unigenes, 89,654 (45.7%), 42,209 (21.5%), and 32,318 (16.5%) were found to be associated with at least one KOG, GO, or KEGG ortholog function. In the early treatment (day 2), the most strongly upregulated genes were those involved in the fatty acid biosynthesis and metabolism and oxidative phosphorylation, whereas the most downregulated genes were those involved in carbohydrate metabolism and photosynthesis. Genes that produce terpenoid liquid hydrocarbons were also well identified and annotated, and 21 (or 29.2%) were differentially expressed along the cobalt treatment.ConclusionsBotryococcus braunii SAG 807-1 can tolerate high cobalt concentration and benefit from hydrocarbon accumulation. The time-course expression profiles for fatty acid biosynthesis, metabolism, and TAG assembly were obtained through different approaches but had equally satisfactory results with the redirection of free long-chain fatty acid and VLCFA away from TAG assembly and oxidation. These molecules served as precursors and backbone supply for the fatty acid-derived hydrocarbon accumulation. These findings provide a foundation for exploiting the regulation mechanisms in B. braunii race A for improved photosynthetic production of hydrocarbons.
SubtypeArticle
KeywordBotryococcus Braunii Cobalt Treatment Physiological Response Transcriptome Regulation Mechanism
DOI10.1186/s13068-018-1331-5
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Technology
Indexed BySCI
Funding OrganizationNational Natural Science Foundation of China(31560724 ; China Postdoctoral Science Foundation(2017T100583 ; Natural Science Foundation of Jiangxi Province(20171BAB214014) ; Natural Science Foundation of Shanghai(18ZR1410100 ; Shanghai Pujiang Program(18PJD008) ; Open Funding Project of the State Key Laboratory of Bioreactor Engineering(2018021) ; Key Laboratory of Poyang Lake Ecological Environment and Resource Development(PK2017001) ; K. C. Wong Magna Fund in Ningbo University ; 31872608) ; 2016M600616) ; 17ZR1406700) ; National Natural Science Foundation of China(31560724 ; China Postdoctoral Science Foundation(2017T100583 ; Natural Science Foundation of Jiangxi Province(20171BAB214014) ; Natural Science Foundation of Shanghai(18ZR1410100 ; Shanghai Pujiang Program(18PJD008) ; Open Funding Project of the State Key Laboratory of Bioreactor Engineering(2018021) ; Key Laboratory of Poyang Lake Ecological Environment and Resource Development(PK2017001) ; K. C. Wong Magna Fund in Ningbo University ; 31872608) ; 2016M600616) ; 17ZR1406700)
Language英语
WOS Research AreaBiotechnology & Applied Microbiology ; Energy & Fuels
WOS SubjectBiotechnology & Applied Microbiology ; Energy & Fuels
WOS KeywordOIL-RICH RACE ; HYDROCARBON PRODUCTION ; SQUALENE SYNTHASE ; GREEN-ALGA ; STRAIN ; GROWTH ; BIOSYNTHESIS ; ACCUMULATION ; CHLOROPHYTA ; MECHANISMS
WOS IDWOS:000453684700002
Funding OrganizationNational Natural Science Foundation of China(31560724 ; China Postdoctoral Science Foundation(2017T100583 ; Natural Science Foundation of Jiangxi Province(20171BAB214014) ; Natural Science Foundation of Shanghai(18ZR1410100 ; Shanghai Pujiang Program(18PJD008) ; Open Funding Project of the State Key Laboratory of Bioreactor Engineering(2018021) ; Key Laboratory of Poyang Lake Ecological Environment and Resource Development(PK2017001) ; K. C. Wong Magna Fund in Ningbo University ; 31872608) ; 2016M600616) ; 17ZR1406700) ; National Natural Science Foundation of China(31560724 ; China Postdoctoral Science Foundation(2017T100583 ; Natural Science Foundation of Jiangxi Province(20171BAB214014) ; Natural Science Foundation of Shanghai(18ZR1410100 ; Shanghai Pujiang Program(18PJD008) ; Open Funding Project of the State Key Laboratory of Bioreactor Engineering(2018021) ; Key Laboratory of Poyang Lake Ecological Environment and Resource Development(PK2017001) ; K. C. Wong Magna Fund in Ningbo University ; 31872608) ; 2016M600616) ; 17ZR1406700)
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/67776
Collection藻类生物技术和生物能源研发中心
Affiliation1.Ningbo Univ, Coll Food & Pharmaceut Sci, Ningbo 315211, Zhejiang, Peoples R China
2.East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China
3.East China Univ Sci & Technol, Dept Appl Biol, Shanghai 200237, Peoples R China
4.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
5.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Qingdao 266101, Peoples R China
6.Jiujiang Univ, Poyang Lake Ecoecon Res Ctr, Jiujiang 332000, Peoples R China
7.Ningbo Univ, Key Lab Marine Biotechnol Zhejiang Prov, 818 Fenghua Rd, Ningbo 315211, Zhejiang, Peoples R China
Recommended Citation
GB/T 7714
Cheng, Pengfei,Zhou, Chengxu,Wang, Yan,et al. Comparative transcriptome analyses of oleaginous Botryococcus braunii race A reveal significant differences in gene expression upon cobalt enrichment[J]. BIOTECHNOLOGY FOR BIOFUELS,2018,11(333):1.
APA Cheng, Pengfei.,Zhou, Chengxu.,Wang, Yan.,Xu, Zhihui.,Xu, Jilin.,...&Fan, Jianhua.(2018).Comparative transcriptome analyses of oleaginous Botryococcus braunii race A reveal significant differences in gene expression upon cobalt enrichment.BIOTECHNOLOGY FOR BIOFUELS,11(333),1.
MLA Cheng, Pengfei,et al."Comparative transcriptome analyses of oleaginous Botryococcus braunii race A reveal significant differences in gene expression upon cobalt enrichment".BIOTECHNOLOGY FOR BIOFUELS 11.333(2018):1.
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