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题名: Identification and characterization of the interferon-gamma-inducible lysosomal thiol reductase gene in Chinese soft-shelled turtle, Pelodiscus sinensis
作者: Fu, Jianping1; Chen, Shannan2; Zhao, Xin1; Luo, Zhang2; Zou, Pengfei2; Liu, Yi1
关键词: GILT ; Thiol reductase activity ; IFN-gamma ; Pelodiscus sinensis
刊名: DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
发表日期: 2019
DOI: 10.1016/j.dci.2018.08.019
卷: 90, 页:55-59
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Fisheries ; Immunology ; Zoology
研究领域[WOS]: Fisheries ; Immunology ; Zoology
英文摘要: The reduction of disulfide bonds of exogenous antigens is crucial to the MHC-II class antigen processing and presenting pathway and is catalysed by interferon-gamma-inducible lysosomal thiol reductase (GILT). In this study, a reptile GILT gene from Chinese soft-shelled turtle, Pelodiscus sinensis (PsGILT), was identified. The full-length cDNA of PsGILT is 1631 nucleotides (nt), including a 5'-untranslated region (UTR) of 3 nt, a 3'-UTR of 860 nt and an open reading frame (ORF) of 768 nt encoding 255 amino acids (aa). The conserved features in known GILTs, such as signal peptide, CXXC motif, GILT signature sequence, N-glycosylation site and conserved cysteines, were all found in the putative PsGILT protein. Genomic analysis revealed that PsGILT kept the "7 exons and 6 introns" structure of vertebrate GILT genes. PsGILT was expressed in all examined organs/tissues and was mainly expressed in spleen and blood. Increased mRNA expression levels of PsIFN-gamma and PsGILT in PBLs were observed after induction with LPS, PolyI:C and recombinant IFN-gamma (rIFN-gamma). We also tested the reductase activity of rGILT in vitro and found that it could reduce intact human IgG into H chains and L chains. These above results implied that PsGILT may play an important role in resisting bacterial and viral infections, like other vertebrate GILTs.
关键词[WOS]: INNATE IMMUNE-RESPONSE ; EXPRESSION ANALYSIS ; GILT GENE ; MOLECULAR-CLONING ; DISULFIDE BONDS ; IFN-GAMMA ; REPLICATION ; MATURATION
语种: 英语
项目资助者: National Natural Science Foundation of China(31502196) ; Natural Science Foundation of Jiangxi Province(20151BAB214021)
WOS记录号: WOS:000449130300007
ISSN号: 0145-305X
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内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/56076
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Jiangxi Normal Univ, Coll Life Sci, 99 Ziyang Rd, Nanchang 330022, Jiangxi, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
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