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Integrated site-specific quantification of faecal bacteria and detection of DNA markers in faecal contamination source tracking as a microbial risk tracking tool in urban Lake ecosystems
Donde, Oscar Omondi1,2,3; Tian Cuicui1; Xiao Bangding1
2018-09-01
Source PublicationJOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN2096-5508
Volume36Issue:5Pages:1629-1642
AbstractThe presence of feacal-derived pathogens in water is responsible for several infectious diseases and deaths worldwide. As a solution, sources of fecal pollution in waters must be accurately assessed, properly determined and strictly controlled. However, the exercise has remained challenging due to the existing overlapping characteristics by different members of faecal coliform bacteria and the inadequacy of information pertaining to the contribution of seasonality and weather condition on tracking the possible sources of pollution. There are continued efforts to improve the Faecal Contamination Source Tracking (FCST) techniques such as Microbial Source Tracking (MST). This study aimed to make contribution to MST by evaluating the efficacy of combining site specific quantification of faecal contamination indicator bacteria and detection of DNA markers while accounting for seasonality and weather conditions' effects in tracking the major sources of faecal contamination in a freshwater system (Donghu Lake, China). The results showed that the use of cyd gene in addition to lacZ and uidA genes differentiates E. coli from other closely related faecal bacteria. The use of selective media increases the pollution source tracking accuracy. BSA addition boosts PCR detection and increases FCST efficiency. Seasonality and weather variability also influence the detection limit for DNA markers.
SubtypeArticle
KeywordAssay Contamination Faecal Bacteria Indicator Source Tracking Water Quality
DOI10.1007/s00343-018-7107-8
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
Indexed BySCI
Funding OrganizationKey Laboratory of Algal Biology of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, People's Republic of China ; Key Laboratory of Algal Biology of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, People's Republic of China ; International College of the University of Chinese Academy of Sciences, Beijing, People's Republic of China ; International College of the University of Chinese Academy of Sciences, Beijing, People's Republic of China ; Egerton University, Department of Environmental Science, Egerton-Kenya ; Egerton University, Department of Environmental Science, Egerton-Kenya ; Kenya Marine and Fisheries Research Institute, Lake Turkana Research Station, Lodwar, Kenya ; Kenya Marine and Fisheries Research Institute, Lake Turkana Research Station, Lodwar, Kenya ; Key Laboratory of Algal Biology of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, People's Republic of China ; Key Laboratory of Algal Biology of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, People's Republic of China ; International College of the University of Chinese Academy of Sciences, Beijing, People's Republic of China ; International College of the University of Chinese Academy of Sciences, Beijing, People's Republic of China ; Egerton University, Department of Environmental Science, Egerton-Kenya ; Egerton University, Department of Environmental Science, Egerton-Kenya ; Kenya Marine and Fisheries Research Institute, Lake Turkana Research Station, Lodwar, Kenya ; Kenya Marine and Fisheries Research Institute, Lake Turkana Research Station, Lodwar, Kenya
Language英语
WOS Research AreaMarine & Freshwater Biology ; Oceanography
WOS SubjectLimnology ; Oceanography
WOS KeywordREAL-TIME PCR ; ESCHERICHIA-COLI ; GENETIC-MARKERS ; QUANTITATIVE PCR ; MULTIPLEX PCR ; WATER ; POLLUTION ; IDENTIFICATION ; INDICATORS ; CATCHMENT
WOS IDWOS:000450165300015
Funding OrganizationKey Laboratory of Algal Biology of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, People's Republic of China ; Key Laboratory of Algal Biology of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, People's Republic of China ; International College of the University of Chinese Academy of Sciences, Beijing, People's Republic of China ; International College of the University of Chinese Academy of Sciences, Beijing, People's Republic of China ; Egerton University, Department of Environmental Science, Egerton-Kenya ; Egerton University, Department of Environmental Science, Egerton-Kenya ; Kenya Marine and Fisheries Research Institute, Lake Turkana Research Station, Lodwar, Kenya ; Kenya Marine and Fisheries Research Institute, Lake Turkana Research Station, Lodwar, Kenya ; Key Laboratory of Algal Biology of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, People's Republic of China ; Key Laboratory of Algal Biology of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, People's Republic of China ; International College of the University of Chinese Academy of Sciences, Beijing, People's Republic of China ; International College of the University of Chinese Academy of Sciences, Beijing, People's Republic of China ; Egerton University, Department of Environmental Science, Egerton-Kenya ; Egerton University, Department of Environmental Science, Egerton-Kenya ; Kenya Marine and Fisheries Research Institute, Lake Turkana Research Station, Lodwar, Kenya ; Kenya Marine and Fisheries Research Institute, Lake Turkana Research Station, Lodwar, Kenya
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/56075
Collection水环境工程研究中心
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Lake Restorat Res Grp, Key Lab Algal Biol, Wuhan 430072, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Int Coll, Beijing 100049, Peoples R China
3.Egerton Univ, Dept Environm Sci, POB 536-20115, Egerton, Kenya
Recommended Citation
GB/T 7714
Donde, Oscar Omondi,Tian Cuicui,Xiao Bangding. Integrated site-specific quantification of faecal bacteria and detection of DNA markers in faecal contamination source tracking as a microbial risk tracking tool in urban Lake ecosystems[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2018,36(5):1629-1642.
APA Donde, Oscar Omondi,Tian Cuicui,&Xiao Bangding.(2018).Integrated site-specific quantification of faecal bacteria and detection of DNA markers in faecal contamination source tracking as a microbial risk tracking tool in urban Lake ecosystems.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,36(5),1629-1642.
MLA Donde, Oscar Omondi,et al."Integrated site-specific quantification of faecal bacteria and detection of DNA markers in faecal contamination source tracking as a microbial risk tracking tool in urban Lake ecosystems".JOURNAL OF OCEANOLOGY AND LIMNOLOGY 36.5(2018):1629-1642.
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