Dietary citric acid supplementation alleviates soybean meal-induced intestinal oxidative damage and micro-ecological imbalance in juvenile turbot, Scophthalmus maximus L
Chen, Zhichu1,2; Zhao, Sifan1,2; Liu, Yang1,2; Yang, Pei1,2; Ai, Qinghui1,2; Zhang, Wenbing1,2; Xu, Weiqi1,2; Zhang, Yongan3; Zhang, Yanjiao1,2,4; Mai, Kangsen1,2,4
2018-12-01
Source PublicationAQUACULTURE RESEARCH
ISSN1355-557X
Volume49Issue:12Pages:3804-3816
AbstractA 12-week feeding trial was conducted to investigate the potential protective effects of citric acid against soybean meal-induced intestinal oxidative damage and micro-ecological imbalance in juvenile turbot (S. maximus L.). Four isonitrogenous and isolipidic experimental diets, that is fish meal-based diet (FM), FM with 40% fish meal protein replaced with soybean meal protein diet (SBM), supplemented with 1.5% citric acid (1.5% CA) or 3% citric acid (3% CA). Results showed that both citric acid diets significantly enhanced the total antioxidative capacity and the gene expression of superoxide dismutase, glutathione peroxidase and heme oxygenase 1, while also decreasing the malondialdehyde content in the distal intestine. Compared to diet SBM, the genes expression of p53, protein kinase C and Caspase-3 were remarkably declined by dietary citric acid supplementation, while the genes expression of proliferating cell nuclear antigen and mucins showed an opposite trend. The structural integrity of the distal intestine in fish fed citric acid was showed in the histological results. Sequencing of bacterial 16s rRNA V4 region showed that the profile of intestinal bacteria was altered by dietary citric acid supplementation, which was supported by the diet-cluster of PCoA and phylogenetic tree. MetaStat analysis indicated that dietary citric acid dramatically reduced the relative abundance of the Vibrio genus. In conclusion, dietary citric acid mitigated soybean meal-induced intestinal oxidative damage, beneficially alleviated the micro-ecological imbalance and specifically reduced the relative abundance of the Vibrio genus in the distal intestine of juvenile turbot.
SubtypeArticle
KeywordCitric Acid Intestinal Barrier Function Oxidative Stress Soybean Meal Turbot
DOI10.1111/are.13847
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationNational Basic Research Program(2014CB138601) ; National Basic Research Program(2014CB138601) ; China Agriculture Research System(CARS 50-G08) ; China Agriculture Research System(CARS 50-G08) ; National Natural Science Foundation of China(41576137 ; National Natural Science Foundation of China(41576137 ; 31202006) ; 31202006) ; National Basic Research Program(2014CB138601) ; National Basic Research Program(2014CB138601) ; China Agriculture Research System(CARS 50-G08) ; China Agriculture Research System(CARS 50-G08) ; National Natural Science Foundation of China(41576137 ; National Natural Science Foundation of China(41576137 ; 31202006) ; 31202006)
Language英语
WOS Research AreaFisheries
WOS SubjectFisheries
WOS KeywordDIGESTIVE ENZYME-ACTIVITIES ; SHRIMP LITOPENAEUS-VANNAMEI ; AIRWAY EPITHELIAL-CELLS ; TILAPIA OREOCHROMIS SP. ; CHAIN FATTY-ACIDS ; SALMO-SALAR L. ; GROWTH-PERFORMANCE ; ORGANIC-ACIDS ; GUT MICROBIOTA ; HOST-DEFENSE
WOS IDWOS:000449876500011
Funding OrganizationNational Basic Research Program(2014CB138601) ; National Basic Research Program(2014CB138601) ; China Agriculture Research System(CARS 50-G08) ; China Agriculture Research System(CARS 50-G08) ; National Natural Science Foundation of China(41576137 ; National Natural Science Foundation of China(41576137 ; 31202006) ; 31202006) ; National Basic Research Program(2014CB138601) ; National Basic Research Program(2014CB138601) ; China Agriculture Research System(CARS 50-G08) ; China Agriculture Research System(CARS 50-G08) ; National Natural Science Foundation of China(41576137 ; National Natural Science Foundation of China(41576137 ; 31202006) ; 31202006)
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/56065
Collection藻类生物学及应用研究中心_水生生物分子与细胞生物学研究中心
Affiliation1.Ocean Univ China, Minist Agr, Key Lab Aquaculture Nutr & Feed, Qingdao, Peoples R China
2.Ocean Univ China, Minist Educ, Key Lab Mariculture, Qingdao, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Hubei, Peoples R China
4.Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao, Peoples R China
Recommended Citation
GB/T 7714
Chen, Zhichu,Zhao, Sifan,Liu, Yang,et al. Dietary citric acid supplementation alleviates soybean meal-induced intestinal oxidative damage and micro-ecological imbalance in juvenile turbot, Scophthalmus maximus L[J]. AQUACULTURE RESEARCH,2018,49(12):3804-3816.
APA Chen, Zhichu.,Zhao, Sifan.,Liu, Yang.,Yang, Pei.,Ai, Qinghui.,...&Mai, Kangsen.(2018).Dietary citric acid supplementation alleviates soybean meal-induced intestinal oxidative damage and micro-ecological imbalance in juvenile turbot, Scophthalmus maximus L.AQUACULTURE RESEARCH,49(12),3804-3816.
MLA Chen, Zhichu,et al."Dietary citric acid supplementation alleviates soybean meal-induced intestinal oxidative damage and micro-ecological imbalance in juvenile turbot, Scophthalmus maximus L".AQUACULTURE RESEARCH 49.12(2018):3804-3816.
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