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Oocyte-specific maternal Slbp2 is required for replication-dependent histone storage and early nuclear cleavage in zebrafish oogenesis and embryogenesis
He, Wen-Xia1,2; Wu, Min1,2; Liu, Zhen1,2; Li, Zhi1; Wang, Yang1,2; Zhou, Jian1,2; Yu, Peng1,2; Zhang, Xiao-Juan1; Zhou, Li1,2; Gui, Jian-Fang1,2
2018-12-01
Source PublicationRNA
ISSN1355-8382
Volume24Issue:12Pages:1738-1748
AbstractStem-loop binding protein (SLBP) is required for replication-dependent histone mRNA metabolism in mammals. Zebrafish possesses two slbps, and slbp1 is necessary for retinal neurogenesis. However, the detailed expression and function of slbp2 in zebrafish are still unknown. In this study, we first identified zebrafish slbp2 as an oocyte-specific maternal factor and then generated a maternal-zygotic slbp2F3 homozygous mutant (MZslbp2 Delta 4(-/-)) using CRISPR/Cas9. The depletion of maternal Slbp2 disrupted early nuclear cleavage, which resulted in developmental arrest at the MBT stage. The developmental defects could be rescued in slbp2transgenic MZslbp2 Delta 4(-/-) embryos. However, homozygous mutant MZslbp1 Delta 1(-/-)developed normally, indicating slbp1 is dispensable for zebrafish early embryogenesis. Through comparative proteome and transcriptome profiling between WT and MZslbp2 Delta 4(-/- )embryos, we identified many differentially expressed proteins and genes. In comparison with those in WT embryos, four replication-dependent histones, including H2a, H2b, H3, and H4, all reduced their expression, while histone variant h2afx significantly increased in MZslbp2 Delta 4(-/- )embryos at the 256-cell stage and high stage. Zebrafish Slbp2 can bind histone mRNA stem-loop in vitro, and the defects of MZslbp2 Delta 4(-/-) embryos can be partially rescued by overexpression of H2b. The current data indicate that maternal Slbp2 plays a pivotal role in the storage of replication-dependent histone mRNAs and proteins during zebrafish oogenesis.
SubtypeArticle
KeywordSlbp2 Replication-dependent Histones Ribosomal Proteins Maternal Effect Oogenesis Early Embryogenesis
DOI10.1261/rna.067090.118
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationKey Program of Frontier Sciences of the Chinese Academy of Sciences(QYZDY-SSW-SMC025) ; Key Program of Frontier Sciences of the Chinese Academy of Sciences(QYZDY-SSW-SMC025) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDA08030201) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDA08030201) ; Earmarked Fund for Modern Agro-industry Technology Research System(NYCYTX-49) ; Earmarked Fund for Modern Agro-industry Technology Research System(NYCYTX-49) ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology(2016FBZ01) ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology(2016FBZ01) ; Key Program of Frontier Sciences of the Chinese Academy of Sciences(QYZDY-SSW-SMC025) ; Key Program of Frontier Sciences of the Chinese Academy of Sciences(QYZDY-SSW-SMC025) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDA08030201) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDA08030201) ; Earmarked Fund for Modern Agro-industry Technology Research System(NYCYTX-49) ; Earmarked Fund for Modern Agro-industry Technology Research System(NYCYTX-49) ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology(2016FBZ01) ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology(2016FBZ01)
Language英语
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemistry & Molecular Biology
WOS KeywordLOOP-BINDING-PROTEIN ; EARLY EMBRYONIC-DEVELOPMENT ; STEM-LOOP ; MESSENGER-RNA ; GENE-EXPRESSION ; H2AX PHOSPHORYLATION ; GENOMIC INSTABILITY ; POLY(A) TAIL ; IN-VIVO ; TRANSLATION
WOS IDWOS:000450373800011
Funding OrganizationKey Program of Frontier Sciences of the Chinese Academy of Sciences(QYZDY-SSW-SMC025) ; Key Program of Frontier Sciences of the Chinese Academy of Sciences(QYZDY-SSW-SMC025) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDA08030201) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDA08030201) ; Earmarked Fund for Modern Agro-industry Technology Research System(NYCYTX-49) ; Earmarked Fund for Modern Agro-industry Technology Research System(NYCYTX-49) ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology(2016FBZ01) ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology(2016FBZ01) ; Key Program of Frontier Sciences of the Chinese Academy of Sciences(QYZDY-SSW-SMC025) ; Key Program of Frontier Sciences of the Chinese Academy of Sciences(QYZDY-SSW-SMC025) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDA08030201) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDA08030201) ; Earmarked Fund for Modern Agro-industry Technology Research System(NYCYTX-49) ; Earmarked Fund for Modern Agro-industry Technology Research System(NYCYTX-49) ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology(2016FBZ01) ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology(2016FBZ01)
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Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/56063
Collection鱼类生物学及渔业生物技术研究中心
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
He, Wen-Xia,Wu, Min,Liu, Zhen,et al. Oocyte-specific maternal Slbp2 is required for replication-dependent histone storage and early nuclear cleavage in zebrafish oogenesis and embryogenesis[J]. RNA,2018,24(12):1738-1748.
APA He, Wen-Xia.,Wu, Min.,Liu, Zhen.,Li, Zhi.,Wang, Yang.,...&Gui, Jian-Fang.(2018).Oocyte-specific maternal Slbp2 is required for replication-dependent histone storage and early nuclear cleavage in zebrafish oogenesis and embryogenesis.RNA,24(12),1738-1748.
MLA He, Wen-Xia,et al."Oocyte-specific maternal Slbp2 is required for replication-dependent histone storage and early nuclear cleavage in zebrafish oogenesis and embryogenesis".RNA 24.12(2018):1738-1748.
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