Characterization of Sexual Trait Development in cyp17a1-Deficient Zebrafish
Zhai, Gang1; Shu, Tingting1,2; Xia, Yuguo3; Lu, Yao1,2; Shang, Guohui1,2; Jin, Xia1; He, Jiangyan1; Nie, Pin1; Yin, Zhan1
2018-10-01
Source PublicationENDOCRINOLOGY
ISSN0013-7227
Volume159Issue:10Pages:3549-3562
AbstractCytochrome P450 (Cyp) 17A1 has both 17 alpha-hydroxylase and 17,20-lyase activities, which are involved in the steroidogenic pathway that produces androgens and estrogens. Previously, a phenotype of all-male cyp17a1-deficient zebrafish generated by transcription activatorlike effector nuclease has been reported. In the current study, the mechanisms relating to Cyp17a1 that are involved in the development of sexual traits, especially gonadal differentiation and testicular development, were characterized. We found that the cyp17a1-deficient fish at 3 months postfertilization (mpf) were all fertile males with normal testis and spermatogenesis but compromised male-typical mating behaviors and secondary sex characters (SSCs), including breeding tubercles, body pigmentation, and anal fin coloration. These results demonstrate that spermatogenesis and testicular development are not as susceptible to androgen deficiency compared with the formation of male-typical SSCs and mating behaviors in zebrafish. The differentiation of the juvenile ovary into the mature ovary failed during the critical sexual differentiation stage. This all-male phenotype of the cyp17a1-deficient fish could be restored with testosterone or estradiol treatment. For testicular development in cyp17a1-deficient fish, a gradually increasing number of spermatozoa and testis hypertrophy from 3 to 6 mpf were observed, accompanied by constitutively upregulated pituitary gonadotropin FSH subunit beta (fsh beta). The hypertrophic testis and enhanced spermatogenesis in the cyp17a1-deficient fish at 6 mpf could be effectively rescued by fshb depletion. These results confirm that adequate estrogen is essential for maintaining ovarian differentiation, and they provide new insight into the role of FSH beta in male testicular development and spermatogenesis.
SubtypeArticle
DOI10.1210/en.2018-00551
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationNational Natural Science Foundation of China(31530077 ; National Natural Science Foundation of China(31530077 ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; National Basic Research Program of China (973 Program)(2014CB138602) ; National Basic Research Program of China (973 Program)(2014CB138602) ; Pilot Program A Project from the Chinese Academy of Sciences(XDA08010405) ; Pilot Program A Project from the Chinese Academy of Sciences(XDA08010405) ; 31501857) ; 31501857) ; National Natural Science Foundation of China(31530077 ; National Natural Science Foundation of China(31530077 ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; National Basic Research Program of China (973 Program)(2014CB138602) ; National Basic Research Program of China (973 Program)(2014CB138602) ; Pilot Program A Project from the Chinese Academy of Sciences(XDA08010405) ; Pilot Program A Project from the Chinese Academy of Sciences(XDA08010405) ; 31501857) ; 31501857)
Language英语
WOS Research AreaEndocrinology & Metabolism
WOS SubjectEndocrinology & Metabolism
WOS KeywordFOLLICLE-STIMULATING-HORMONE ; ANTI-MULLERIAN-HORMONE ; GENDER-ROLE BEHAVIOR ; TARGETED DISRUPTION ; DANIO-RERIO ; OVARIAN-DIFFERENTIATION ; AROMATASE INHIBITOR ; ANDROGEN RECEPTOR ; ORYZIAS-LATIPES ; ADULT ZEBRAFISH
WOS IDWOS:000446428300008
Funding OrganizationNational Natural Science Foundation of China(31530077 ; National Natural Science Foundation of China(31530077 ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; National Basic Research Program of China (973 Program)(2014CB138602) ; National Basic Research Program of China (973 Program)(2014CB138602) ; Pilot Program A Project from the Chinese Academy of Sciences(XDA08010405) ; Pilot Program A Project from the Chinese Academy of Sciences(XDA08010405) ; 31501857) ; 31501857) ; National Natural Science Foundation of China(31530077 ; National Natural Science Foundation of China(31530077 ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; National Basic Research Program of China (973 Program)(2014CB138602) ; National Basic Research Program of China (973 Program)(2014CB138602) ; Pilot Program A Project from the Chinese Academy of Sciences(XDA08010405) ; Pilot Program A Project from the Chinese Academy of Sciences(XDA08010405) ; 31501857) ; 31501857)
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/56049
Collection藻类生物学及应用研究中心_水生生物分子与细胞生物学研究中心
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing 101408, Peoples R China
3.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Guangzhou 510380, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
Zhai, Gang,Shu, Tingting,Xia, Yuguo,et al. Characterization of Sexual Trait Development in cyp17a1-Deficient Zebrafish[J]. ENDOCRINOLOGY,2018,159(10):3549-3562.
APA Zhai, Gang.,Shu, Tingting.,Xia, Yuguo.,Lu, Yao.,Shang, Guohui.,...&Yin, Zhan.(2018).Characterization of Sexual Trait Development in cyp17a1-Deficient Zebrafish.ENDOCRINOLOGY,159(10),3549-3562.
MLA Zhai, Gang,et al."Characterization of Sexual Trait Development in cyp17a1-Deficient Zebrafish".ENDOCRINOLOGY 159.10(2018):3549-3562.
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