IHB OpenIR  > 藻类生物学及应用研究中心
A protocol for efficient callus induction and stable transformation of Spirodela polyrhiza (L.) Schleiden using Agrobacterium tumefaciens
Yang, Jingjing1,2,3; Lia, Gaojie1,2,3; Hua, Shiqi1,2,3; Bishopp, Antony4; Heenatigala, P. P. M.1,2,3; Kumar, Sunjeet1,2,3; Duan, Pengfei5; Yao, Lunguang5; Hou, Hongwei1,2,3
2018-12-01
Source PublicationAQUATIC BOTANY
ISSN0304-3770
Volume151Pages:80-86
AbstractSpirodela polyrhiza represents the largest specie with the smallest genome of all the members of the Lemnoideae. Its genome features have been delineated, revealing its fewest predicted genes of any known plant genome. It is also ideal system for basic biological researches and various practical applications including toxicity testing, bioreactor, biomonitoring and biofuel. In this study, we reported the successful induction of S. polyrhiza callus coupled with the efficient stable transformation using the Agrobacterium tumefaciens strain LBA4404 by optimizing each step of the process. We found that the highest callus induction efficiency was achieved with 22.62 uM 2,4-D and 8.88 mu M 6-BA, with above 90% of fronds forming calli. We also determined that 100 M acetosyringone in the co-cultivation medium and the maintenance of pH value at 5.2 were crucial for high transformation efficiency (up to 13 1.5%). As proof of concept, we transformed S. polyrhiza with the DR5 and TCS synthetic reporters, which have previously been used to report cytokinin and auxin signaling output in the model plant Arabidopsis thaliana. The cytokinin showed highest accumulation at the initial stage of bud formation and the frond apex of S. polyrhiza whilst the expression of auxin was observed highest at frond with middle size. These transformed lines provide an effective way to investigate the development of S. polyrhiza and may shed light on the interesting way in which this specie reproduces. This is the first report of highly efficient callus induction and Agrobacterium tumefaciens-mediated transformation in S. polyrhiza.
SubtypeArticle
KeywordSpirodela Polyrhiza Callus Induction Transformation Agrobacterium Tumefaciens Cytokinin Auxin
DOI10.1016/j.aquabot.2018.08.004
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationState Key Laboratory of Freshwater Ecology and Biotechnology(2016FB04) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2016FB04) ; Project of Major Science and Technology Program for Water Pollution Control and Treatment(2015ZX07503-005) ; Project of Major Science and Technology Program for Water Pollution Control and Treatment(2015ZX07503-005) ; Major Project of Technology Innovation Program of Hubei, China(2017ABA135) ; Major Project of Technology Innovation Program of Hubei, China(2017ABA135) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2016FB04) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2016FB04) ; Project of Major Science and Technology Program for Water Pollution Control and Treatment(2015ZX07503-005) ; Project of Major Science and Technology Program for Water Pollution Control and Treatment(2015ZX07503-005) ; Major Project of Technology Innovation Program of Hubei, China(2017ABA135) ; Major Project of Technology Innovation Program of Hubei, China(2017ABA135)
Language英语
WOS Research AreaPlant Sciences ; Marine & Freshwater Biology
WOS SubjectPlant Sciences ; Marine & Freshwater Biology
WOS KeywordLEMNA-MINOR ; ARABIDOPSIS-THALIANA ; PLANT-REGENERATION ; DUCKWEED ; GIBBA ; GENE ; PHARMACEUTICALS ; RHIZOSPHERE ; EXPRESSION ; COMPONENTS
WOS IDWOS:000448091000012
Funding OrganizationState Key Laboratory of Freshwater Ecology and Biotechnology(2016FB04) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2016FB04) ; Project of Major Science and Technology Program for Water Pollution Control and Treatment(2015ZX07503-005) ; Project of Major Science and Technology Program for Water Pollution Control and Treatment(2015ZX07503-005) ; Major Project of Technology Innovation Program of Hubei, China(2017ABA135) ; Major Project of Technology Innovation Program of Hubei, China(2017ABA135) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2016FB04) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2016FB04) ; Project of Major Science and Technology Program for Water Pollution Control and Treatment(2015ZX07503-005) ; Project of Major Science and Technology Program for Water Pollution Control and Treatment(2015ZX07503-005) ; Major Project of Technology Innovation Program of Hubei, China(2017ABA135) ; Major Project of Technology Innovation Program of Hubei, China(2017ABA135)
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/56036
Collection藻类生物学及应用研究中心
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Hubei, Peoples R China
3.Univ Chinese Acad Sci, Wuhan 430072, Hubei, Peoples R China
4.Univ Nottingham, Ctr Plant Integrat Biol, Nottingham, England
5.Nanyang Normal Univ, Coll Agr Engn, Collaborat Innovat Ctr Water Secur Water Source R, Nanyang, Henan, Peoples R China
Recommended Citation
GB/T 7714
Yang, Jingjing,Lia, Gaojie,Hua, Shiqi,et al. A protocol for efficient callus induction and stable transformation of Spirodela polyrhiza (L.) Schleiden using Agrobacterium tumefaciens[J]. AQUATIC BOTANY,2018,151:80-86.
APA Yang, Jingjing.,Lia, Gaojie.,Hua, Shiqi.,Bishopp, Antony.,Heenatigala, P. P. M..,...&Hou, Hongwei.(2018).A protocol for efficient callus induction and stable transformation of Spirodela polyrhiza (L.) Schleiden using Agrobacterium tumefaciens.AQUATIC BOTANY,151,80-86.
MLA Yang, Jingjing,et al."A protocol for efficient callus induction and stable transformation of Spirodela polyrhiza (L.) Schleiden using Agrobacterium tumefaciens".AQUATIC BOTANY 151(2018):80-86.
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