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题名: Global and Complement Gene-Specific DNA Methylation in Grass Carp after Grass Carp Reovirus (GCRV) Infection
作者: Xiong, Lv1, 2; He, Libo1; Luo, Lifei1, 2; Li, Yongming1; Liao, Lanjie1; Huang, Rong1; Zhu, Zuoyan1; Wang, Yaping1
关键词: grass carp ; grass carp reovirus ; DNA methylation ; gene expression ; complement component 3 ; kininogen-1 ; negative correlation
刊名: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
发表日期: 2018-04-01
DOI: 10.3390/ijms19041110
卷: 19, 期:4
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]: Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
英文摘要: Grass carp reovirus (GCRV) causes huge economic loss to the grass carp cultivation industry but the mechanism remains largely unknown. In this study, we investigated the global and complement gene-specific DNA methylation in grass carp after GCRV infection aimed to uncover the mechanism underlying GCRV infection. The global DNA methylation level was increased after GCRV infection. Expression levels of enzymes involved in DNA methylation including DNA methyltransferase (DNMT), ten-eleven translocation proteins (TETs), and glycine N-methyltransferase (GNMT) were significantly altered after GCRV infection. In order to investigate the relationship between the gene expression level and DNA methylation level, two representative complement genes, complement component 3 (C3) and kininogen-1 (KNG1), were selected for further analysis. mRNA expression levels of the two genes were significantly increased at 5 and 7 days after GCRV infection, whereas the DNA methylation level at the 5 0 flanking regions of the two genes were down-regulated at the same time-points. Moreover, a negative correlation was detected between gene expression levels and DNA methylation levels of the two genes. Therefore, the current data revealed a global and complement gene-specific DNA methylation profile after GCRV infection. Our study would provide new insights into understanding the mechanism underlying GCRV infection.
关键词[WOS]: MESSENGER-RNA EXPRESSION ; GLYCINE N-METHYLTRANSFERASE ; CTENOPHARYNGODON-IDELLA ; PROKARYOTIC EXPRESSION ; GENOME ; MECHANISM ; DISEASE ; PROTEIN ; EPIGENETICS ; RESISTANCE
语种: 英语
项目资助者: National Natural Science Foundation of China(31721005 ; Strategic Priority Research Programme of Chinese Academy of Sciences(XDA08030203) ; Hubei Natural Science Foundation of China(2015CFA007) ; 31702332)
WOS记录号: WOS:000434978700189
ISSN号: 1422-0067
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/55838
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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