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题名: Development of Efficient Protocols for Stable and Transient Gene Transformation for Wolffia Globosa Using Agrobacterium
作者: Heenatigala, P. P. M.1, 2; Yang, Jingjing1; Bishopp, Anthony3; Sun, Zuoliang1; Li, Gaojie1; Kumar, Sunjeet1; Hu, Shiqi1; Wu, Zhigang1; Lin, Wei1; Yao, Lunguang4; Duan, Pengfei; Hou, Hongwei1
关键词: transient transformation ; stable transformation ; duckweed ; Wolffia globosa ; auxin ; cytokinin
刊名: FRONTIERS IN CHEMISTRY
发表日期: 2018-06-21
DOI: 10.3389/fchem.2018.00227
卷: 6
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Physical Sciences
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
英文摘要: Members of the Wolffia genus are fascinating plants for many biologists as they are the smallest flowering plants on Earth and exhibit a reduced body plan that is of great interest to developmental biologists. There has also been recent interest in the use of these species for bioenergy or biorefining. Molecular and developmental studies have been limited in Wolffia species due to the high genome complexity and uncertainties regarding the stable genetic transformation. In this manuscript we present new protocols for both stable and transient genetic transformation for Wolffia globosa using Agrobacterium tumefaciens. For the transient transformation, we used Wolffia fronds whereas we used clusters for the stable transformation. As proof of concept we transformed two synthetic promoter constructs driving expression of the GUS marker gene, that have previously been used to monitor auxin and cytokinin output in a variety of species. Using these approaches we obtained a Transformation Efficiency (TE) of 0.14% for the stable transformation and 21.8% for the transient transformation. The efficiency of these two methods of transformation are sufficient to allow future studies to investigate gene function. This is the first report for successful stable transformation of W. globosa.
关键词[WOS]: MEDIATED TRANSFORMATION ; AQUATIC PLANTS ; DUCKWEED ; LEMNACEAE ; COLUMBIANA ; EXPRESSION ; INDUCTION ; SYSTEMS ; ARRHIZA ; CALLUS
语种: 英语
项目资助者: Project of State Key Laboratory of Freshwater Ecology and Biotechnology China(2016FB04) ; project of Natural Science Foundation of Hubei Province(2015CFB488) ; Scientific research and service platform fund of Henan Province(2016151) ; fund of scientific and technological innovation team of water ecological security for Water Source Region of Mid-line of South-to-North Diversion Project of Henan Province(17454) ; Hubei province technical innovation special (major project)(2017ABA135)
WOS记录号: WOS:000435756900001
ISSN号: 2296-2646
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内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/55833
Appears in Collections:水生生物分子与细胞生物学研究中心_期刊论文

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作者单位: 1.Univ Chinese Acad Sci, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Key Lab Aquat Biodivers & Conservat,Inst Hydrobio, Wuhan, Hubei, Peoples R China
2.Natl Aquat Resources Res & Dev Agcy, Inland Aquat Resources & Aquaculture Div, Colombo, Sri Lanka
3.Univ Nottingham, Ctr Plant Integrat Biol, Nottingham, England
4.Nanyang Normal Univ, Coll Agr Engn, Collaborat Innovat Ctr Water Secur Water Source R, Nanyang, Peoples R China
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