Development of Efficient Protocols for Stable and Transient Gene Transformation for Wolffia Globosa Using Agrobacterium
Heenatigala, P. P. M.1,2; Yang, Jingjing1; Bishopp, Anthony3; Sun, Zuoliang1; Li, Gaojie1; Kumar, Sunjeet1; Hu, Shiqi1; Wu, Zhigang1; Lin, Wei1; Yao, Lunguang4; Duan, Pengfei; Hou, Hongwei1
2018-06-21
Source PublicationFRONTIERS IN CHEMISTRY
ISSN2296-2646
Volume6
AbstractMembers of the Wolffia genus are fascinating plants for many biologists as they are the smallest flowering plants on Earth and exhibit a reduced body plan that is of great interest to developmental biologists. There has also been recent interest in the use of these species for bioenergy or biorefining. Molecular and developmental studies have been limited in Wolffia species due to the high genome complexity and uncertainties regarding the stable genetic transformation. In this manuscript we present new protocols for both stable and transient genetic transformation for Wolffia globosa using Agrobacterium tumefaciens. For the transient transformation, we used Wolffia fronds whereas we used clusters for the stable transformation. As proof of concept we transformed two synthetic promoter constructs driving expression of the GUS marker gene, that have previously been used to monitor auxin and cytokinin output in a variety of species. Using these approaches we obtained a Transformation Efficiency (TE) of 0.14% for the stable transformation and 21.8% for the transient transformation. The efficiency of these two methods of transformation are sufficient to allow future studies to investigate gene function. This is the first report for successful stable transformation of W. globosa.
SubtypeArticle
KeywordTransient Transformation Stable Transformation Duckweed Wolffia Globosa Auxin Cytokinin
DOI10.3389/fchem.2018.00227
WOS HeadingsScience & Technology ; Physical Sciences
Indexed BySCI
Funding OrganizationProject of State Key Laboratory of Freshwater Ecology and Biotechnology China(2016FB04) ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology China(2016FB04) ; project of Natural Science Foundation of Hubei Province(2015CFB488) ; project of Natural Science Foundation of Hubei Province(2015CFB488) ; Scientific research and service platform fund of Henan Province(2016151) ; Scientific research and service platform fund of Henan Province(2016151) ; fund of scientific and technological innovation team of water ecological security for Water Source Region of Mid-line of South-to-North Diversion Project of Henan Province(17454) ; fund of scientific and technological innovation team of water ecological security for Water Source Region of Mid-line of South-to-North Diversion Project of Henan Province(17454) ; Hubei province technical innovation special (major project)(2017ABA135) ; Hubei province technical innovation special (major project)(2017ABA135) ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology China(2016FB04) ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology China(2016FB04) ; project of Natural Science Foundation of Hubei Province(2015CFB488) ; project of Natural Science Foundation of Hubei Province(2015CFB488) ; Scientific research and service platform fund of Henan Province(2016151) ; Scientific research and service platform fund of Henan Province(2016151) ; fund of scientific and technological innovation team of water ecological security for Water Source Region of Mid-line of South-to-North Diversion Project of Henan Province(17454) ; fund of scientific and technological innovation team of water ecological security for Water Source Region of Mid-line of South-to-North Diversion Project of Henan Province(17454) ; Hubei province technical innovation special (major project)(2017ABA135) ; Hubei province technical innovation special (major project)(2017ABA135)
Language英语
WOS Research AreaChemistry
WOS SubjectChemistry, Multidisciplinary
WOS KeywordMEDIATED TRANSFORMATION ; AQUATIC PLANTS ; DUCKWEED ; LEMNACEAE ; COLUMBIANA ; EXPRESSION ; INDUCTION ; SYSTEMS ; ARRHIZA ; CALLUS
WOS IDWOS:000435756900001
Funding OrganizationProject of State Key Laboratory of Freshwater Ecology and Biotechnology China(2016FB04) ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology China(2016FB04) ; project of Natural Science Foundation of Hubei Province(2015CFB488) ; project of Natural Science Foundation of Hubei Province(2015CFB488) ; Scientific research and service platform fund of Henan Province(2016151) ; Scientific research and service platform fund of Henan Province(2016151) ; fund of scientific and technological innovation team of water ecological security for Water Source Region of Mid-line of South-to-North Diversion Project of Henan Province(17454) ; fund of scientific and technological innovation team of water ecological security for Water Source Region of Mid-line of South-to-North Diversion Project of Henan Province(17454) ; Hubei province technical innovation special (major project)(2017ABA135) ; Hubei province technical innovation special (major project)(2017ABA135) ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology China(2016FB04) ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology China(2016FB04) ; project of Natural Science Foundation of Hubei Province(2015CFB488) ; project of Natural Science Foundation of Hubei Province(2015CFB488) ; Scientific research and service platform fund of Henan Province(2016151) ; Scientific research and service platform fund of Henan Province(2016151) ; fund of scientific and technological innovation team of water ecological security for Water Source Region of Mid-line of South-to-North Diversion Project of Henan Province(17454) ; fund of scientific and technological innovation team of water ecological security for Water Source Region of Mid-line of South-to-North Diversion Project of Henan Province(17454) ; Hubei province technical innovation special (major project)(2017ABA135) ; Hubei province technical innovation special (major project)(2017ABA135)
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/55833
Collection藻类生物学及应用研究中心_水生生物分子与细胞生物学研究中心
Affiliation1.Univ Chinese Acad Sci, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Key Lab Aquat Biodivers & Conservat,Inst Hydrobio, Wuhan, Hubei, Peoples R China
2.Natl Aquat Resources Res & Dev Agcy, Inland Aquat Resources & Aquaculture Div, Colombo, Sri Lanka
3.Univ Nottingham, Ctr Plant Integrat Biol, Nottingham, England
4.Nanyang Normal Univ, Coll Agr Engn, Collaborat Innovat Ctr Water Secur Water Source R, Nanyang, Peoples R China
Recommended Citation
GB/T 7714
Heenatigala, P. P. M.,Yang, Jingjing,Bishopp, Anthony,et al. Development of Efficient Protocols for Stable and Transient Gene Transformation for Wolffia Globosa Using Agrobacterium[J]. FRONTIERS IN CHEMISTRY,2018,6.
APA Heenatigala, P. P. M..,Yang, Jingjing.,Bishopp, Anthony.,Sun, Zuoliang.,Li, Gaojie.,...&Hou, Hongwei.(2018).Development of Efficient Protocols for Stable and Transient Gene Transformation for Wolffia Globosa Using Agrobacterium.FRONTIERS IN CHEMISTRY,6.
MLA Heenatigala, P. P. M.,et al."Development of Efficient Protocols for Stable and Transient Gene Transformation for Wolffia Globosa Using Agrobacterium".FRONTIERS IN CHEMISTRY 6(2018).
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