IHB OpenIR  > 藻类生物学及应用研究中心
Functional Overlap of hetP and hetZ in Regulation of Heterocyst Differentiation in Anabaena sp Strain PCC 7120
Zhang, He1; Wang, Shuai1; Wang, Yali1,2; Xu, Xudong1,2
2018-05-01
Source PublicationJOURNAL OF BACTERIOLOGY
ISSN0021-9193
Volume200Issue:9
AbstractHetR plays a key role in regulation of heterocyst differentiation and patterning in Anabaena. It directly regulates genes involved in heterocyst differentiation (such as hetP and hetZ), genes involved in pattern formation (patA), and many others. In this study, we investigated the functional relationship of hetP and hetZ and their role in HetR-dependent cell differentiation. Coexpression of hetP and hetZ from the promoter of ntcA, which encodes the global nitrogen regulator, enabled a hetR mutant to form heterocysts with low aerobic nitrogenase activity. Overexpression of hetZ restored heterocyst differentiation in a hetP mutant and vice versa. Overexpression of hetR restored heterocyst formation in either a hetP or a hetZ mutant but not in a hetZ hetP double mutant. The functional overlap of hetP and hetZ was further confirmed by reverse transcription-quantitative PCR (RT-qPCR) and transcriptomic analyses of their effects on gene expression. In addition, yeast two-hybrid and pulldown assays showed the interaction of HetZ with HetR. HetP and HetZ are proposed as the two major factors that control heterocyst formation in response to upregulation of hetR.
SubtypeArticle
KeywordAnabaena Heterocyst Differentiation Regulatory Circuit
DOI10.1128/JB.00707-17
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationNational Natural Science Foundation of China(31270132 ; National Natural Science Foundation of China(31270132 ; State Key Laboratory of Freshwater Ecology and Biotechnology at the Institute of Hydrobiology, Chinese Academy of Sciences(2016FBZ09) ; State Key Laboratory of Freshwater Ecology and Biotechnology at the Institute of Hydrobiology, Chinese Academy of Sciences(2016FBZ09) ; 31770044) ; 31770044) ; National Natural Science Foundation of China(31270132 ; National Natural Science Foundation of China(31270132 ; State Key Laboratory of Freshwater Ecology and Biotechnology at the Institute of Hydrobiology, Chinese Academy of Sciences(2016FBZ09) ; State Key Laboratory of Freshwater Ecology and Biotechnology at the Institute of Hydrobiology, Chinese Academy of Sciences(2016FBZ09) ; 31770044) ; 31770044)
Language英语
WOS Research AreaMicrobiology
WOS SubjectMicrobiology
WOS KeywordTRANSCRIPTION FACTOR HETR ; NITROGEN-FIXATION ; CELL-DIFFERENTIATION ; PCC-7120 ; GENE ; EXPRESSION ; CYANOBACTERIA ; METABOLISM ; EVOLUTION ; SELECTION
WOS IDWOS:000429425200009
Funding OrganizationNational Natural Science Foundation of China(31270132 ; National Natural Science Foundation of China(31270132 ; State Key Laboratory of Freshwater Ecology and Biotechnology at the Institute of Hydrobiology, Chinese Academy of Sciences(2016FBZ09) ; State Key Laboratory of Freshwater Ecology and Biotechnology at the Institute of Hydrobiology, Chinese Academy of Sciences(2016FBZ09) ; 31770044) ; 31770044) ; National Natural Science Foundation of China(31270132 ; National Natural Science Foundation of China(31270132 ; State Key Laboratory of Freshwater Ecology and Biotechnology at the Institute of Hydrobiology, Chinese Academy of Sciences(2016FBZ09) ; State Key Laboratory of Freshwater Ecology and Biotechnology at the Institute of Hydrobiology, Chinese Academy of Sciences(2016FBZ09) ; 31770044) ; 31770044)
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/50948
Collection藻类生物学及应用研究中心
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Hubei, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Zhang, He,Wang, Shuai,Wang, Yali,et al. Functional Overlap of hetP and hetZ in Regulation of Heterocyst Differentiation in Anabaena sp Strain PCC 7120[J]. JOURNAL OF BACTERIOLOGY,2018,200(9).
APA Zhang, He,Wang, Shuai,Wang, Yali,&Xu, Xudong.(2018).Functional Overlap of hetP and hetZ in Regulation of Heterocyst Differentiation in Anabaena sp Strain PCC 7120.JOURNAL OF BACTERIOLOGY,200(9).
MLA Zhang, He,et al."Functional Overlap of hetP and hetZ in Regulation of Heterocyst Differentiation in Anabaena sp Strain PCC 7120".JOURNAL OF BACTERIOLOGY 200.9(2018).
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhang, He]'s Articles
[Wang, Shuai]'s Articles
[Wang, Yali]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhang, He]'s Articles
[Wang, Shuai]'s Articles
[Wang, Yali]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhang, He]'s Articles
[Wang, Shuai]'s Articles
[Wang, Yali]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.