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题名: Functional Overlap of hetP and hetZ in Regulation of Heterocyst Differentiation in Anabaena sp Strain PCC 7120
作者: Zhang, He1; Wang, Shuai1; Wang, Yali1, 2; Xu, Xudong1, 2
关键词: Anabaena ; heterocyst differentiation ; regulatory circuit
刊名: JOURNAL OF BACTERIOLOGY
发表日期: 2018-05-01
DOI: 10.1128/JB.00707-17
卷: 200, 期:9
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Microbiology
研究领域[WOS]: Microbiology
英文摘要: HetR plays a key role in regulation of heterocyst differentiation and patterning in Anabaena. It directly regulates genes involved in heterocyst differentiation (such as hetP and hetZ), genes involved in pattern formation (patA), and many others. In this study, we investigated the functional relationship of hetP and hetZ and their role in HetR-dependent cell differentiation. Coexpression of hetP and hetZ from the promoter of ntcA, which encodes the global nitrogen regulator, enabled a hetR mutant to form heterocysts with low aerobic nitrogenase activity. Overexpression of hetZ restored heterocyst differentiation in a hetP mutant and vice versa. Overexpression of hetR restored heterocyst formation in either a hetP or a hetZ mutant but not in a hetZ hetP double mutant. The functional overlap of hetP and hetZ was further confirmed by reverse transcription-quantitative PCR (RT-qPCR) and transcriptomic analyses of their effects on gene expression. In addition, yeast two-hybrid and pulldown assays showed the interaction of HetZ with HetR. HetP and HetZ are proposed as the two major factors that control heterocyst formation in response to upregulation of hetR.
关键词[WOS]: TRANSCRIPTION FACTOR HETR ; NITROGEN-FIXATION ; CELL-DIFFERENTIATION ; PCC-7120 ; GENE ; EXPRESSION ; CYANOBACTERIA ; METABOLISM ; EVOLUTION ; SELECTION
语种: 英语
项目资助者: National Natural Science Foundation of China(31270132 ; State Key Laboratory of Freshwater Ecology and Biotechnology at the Institute of Hydrobiology, Chinese Academy of Sciences(2016FBZ09) ; 31770044)
WOS记录号: WOS:000429425200009
ISSN号: 0021-9193
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内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/50948
Appears in Collections:藻类生物学及应用研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Hubei, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan, Hubei, Peoples R China
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