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A fluorescence ratio-based method to determine microalgal viability and its application to rapid optimization of cryopreservation
Zheng, Lingling1; Lu, Zhe1,2; Zhang, Qi1; Li, Tianli1; Song, Lirong1
2018-04-01
Source PublicationCRYOBIOLOGY
ISSN0011-2240
Volume81Pages:27-33
AbstractThe utility of microalgal biomass and bioproducts depends on long-term maintenance of certain physiological or biochemical features of the species. While unique characteristics may not be durably maintained with general subculture, cryopreservation methods better prevent alterations from desired characteristics. Post-thaw viability is critical to establishing microalgal cultures, and there is a critical need to effectively and rapidly evaluate microalgal viability after the post-thawing process. In the present study, we developed a rapid assay based on the change of fluorescence ratio to determine microalgal viability post-thaw. It was shown that the assessment of microalgal viability by the fluorescence ratio method correlated well with that of the FDA-staining (R-2 = 0.978) and regrowth method (R-2 = 0.976), demonstrating that the present method could be applied in the high throughput detection of viability of microalgal strains. Subsequent to establishing this method, we aimed to find out optimal cryopreservation protocol for each strain from a group of 125 microalgal strains. The viability of these strains under different treatments was quickly evaluated by the fluorescence ratio method. Of these strains, 95 attained post-thaw viability over 60%. DMSO was a suitable cryoprotectant for most strains at a concentration <= 10%. Based on the dataset, the relative contribution of 3 variables-genus, cryoprotectants and concentration to post-viability was analyzed with the Random Forest (RF) classification method. All variables together could explain 97.8% of the viability, and type and concentration of cryoprotectant could explain 59.1% in Chlorophyta. This study provided a new approach for viability assay and demonstrated that this method can facilitate to find, out the optimal protocols for cryopreservation of microalgal strains.
SubtypeArticle
KeywordCryopreservation Economically Important Microalgae Cryoprotectant Concentration Random Forest Fluorescence Ratio Based Method High-throughput Detection
DOI10.1016/j.cryobiol.2018.02.014
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationState Development & Investment Corporation in China(Y341151Z09) ; State Development & Investment Corporation in China(Y341151Z09) ; China Agriculture Research System(CARS-50) ; China Agriculture Research System(CARS-50) ; Chinese Academy of Sciences(ZSSB-006) ; Chinese Academy of Sciences(ZSSB-006) ; State Development & Investment Corporation in China(Y341151Z09) ; State Development & Investment Corporation in China(Y341151Z09) ; China Agriculture Research System(CARS-50) ; China Agriculture Research System(CARS-50) ; Chinese Academy of Sciences(ZSSB-006) ; Chinese Academy of Sciences(ZSSB-006)
Language英语
WOS Research AreaLife Sciences & Biomedicine - Other Topics ; Physiology
WOS SubjectBiology ; Physiology
WOS KeywordCHLOROPHYLL FLUORESCENCE ; ALGAE ; EFFICIENCY ; STRAINS ; CULTURE ; CELLS
WOS IDWOS:000429633800005
Funding OrganizationState Development & Investment Corporation in China(Y341151Z09) ; State Development & Investment Corporation in China(Y341151Z09) ; China Agriculture Research System(CARS-50) ; China Agriculture Research System(CARS-50) ; Chinese Academy of Sciences(ZSSB-006) ; Chinese Academy of Sciences(ZSSB-006) ; State Development & Investment Corporation in China(Y341151Z09) ; State Development & Investment Corporation in China(Y341151Z09) ; China Agriculture Research System(CARS-50) ; China Agriculture Research System(CARS-50) ; Chinese Academy of Sciences(ZSSB-006) ; Chinese Academy of Sciences(ZSSB-006)
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/50939
Collection藻类生物学及应用研究中心
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Zheng, Lingling,Lu, Zhe,Zhang, Qi,et al. A fluorescence ratio-based method to determine microalgal viability and its application to rapid optimization of cryopreservation[J]. CRYOBIOLOGY,2018,81:27-33.
APA Zheng, Lingling,Lu, Zhe,Zhang, Qi,Li, Tianli,&Song, Lirong.(2018).A fluorescence ratio-based method to determine microalgal viability and its application to rapid optimization of cryopreservation.CRYOBIOLOGY,81,27-33.
MLA Zheng, Lingling,et al."A fluorescence ratio-based method to determine microalgal viability and its application to rapid optimization of cryopreservation".CRYOBIOLOGY 81(2018):27-33.
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