IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心
Efficient Gene Transfer and Gene Editing in Sterlet (Acipenser ruthenus)
Chen, Ji1; Wang, Wei2; Tian, Zhaohui2; Dong, Ying2; Dong, Tian2; Zhu, Hua2; Zhu, Zuoyan1; Hu, Hongxia2; Hu, Wei1,3
2018-04-06
Source PublicationFRONTIERS IN GENETICS
ISSN1664-8021
Volume9
AbstractThe sturgeon (Acipenseriformes) is an important farmed species because of its economical value. However, neither gene transfer nor gene editing techniques have been established in sturgeon for molecular breeding and gene functional study until now. In this study, we accomplished gene transfer and gene editing in sterlet (Acipenser ruthenus), which has the shortest sexual maturation period of sturgeons. The plasmid encoding enhanced green fluorescent protein (EGFP) was transferred into the embryos of sterlet at injection concentration of 100 ng/mu L, under which condition high survival rate and gene transfer rate could be achieved. Subsequently, exogenous EGFP was efficiently disrupted by transcription activator-like effector nucleases (TALENs) or clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 nuclease/guide RNA (gRNA), with injection concentrations of 300 ng/mu L TALENs, or 100 ng/mu L Cas9 nuclease and 30 ng/mu L gRNA, respectively, under which condition high survival rate and gene mutation rate could be achieved. Finally, the endogenous gene no tail in sterlet was successfully mutated by Cas9 nuclease/gRNA. We observed the CRISPR-induced no tail mutation, at a high efficiency with the mutant P0 embryos displaying the expected phenotype of bent spine and twisted tail.
SubtypeArticle
KeywordSterlet Gene Transfer Gene Editing Egfp No Tail
DOI10.3389/fgene2018.00117
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationNational Natural Science Foundation of China(31721005 ; National Natural Science Foundation of China(31721005 ; Foundation of Beijing Municipal Science and Technology Project(Z161100004516003) ; Foundation of Beijing Municipal Science and Technology Project(Z161100004516003) ; Innovation Team of Sturgeon and Salmonid of Beijing Project(BAIC08-2018) ; Innovation Team of Sturgeon and Salmonid of Beijing Project(BAIC08-2018) ; Innovation Team of Sturgeon and Salmonid of Baafs Project(JNKST201611) ; Innovation Team of Sturgeon and Salmonid of Baafs Project(JNKST201611) ; Foundation of Beijing Academy of Agriculture and Forestry Sciences: Prospective study of new technology on sturgeon industry ; Foundation of Beijing Academy of Agriculture and Forestry Sciences: Prospective study of new technology on sturgeon industry ; 31325026) ; 31325026) ; National Natural Science Foundation of China(31721005 ; National Natural Science Foundation of China(31721005 ; Foundation of Beijing Municipal Science and Technology Project(Z161100004516003) ; Foundation of Beijing Municipal Science and Technology Project(Z161100004516003) ; Innovation Team of Sturgeon and Salmonid of Beijing Project(BAIC08-2018) ; Innovation Team of Sturgeon and Salmonid of Beijing Project(BAIC08-2018) ; Innovation Team of Sturgeon and Salmonid of Baafs Project(JNKST201611) ; Innovation Team of Sturgeon and Salmonid of Baafs Project(JNKST201611) ; Foundation of Beijing Academy of Agriculture and Forestry Sciences: Prospective study of new technology on sturgeon industry ; Foundation of Beijing Academy of Agriculture and Forestry Sciences: Prospective study of new technology on sturgeon industry ; 31325026) ; 31325026)
Language英语
WOS Research AreaGenetics & Heredity
WOS SubjectGenetics & Heredity
WOS KeywordTROUT ONCORHYNCHUS-MYKISS ; ZINC-FINGER NUCLEASES ; SEX-DETERMINING GENE ; CRISPR-CAS SYSTEM ; TARGETED MUTAGENESIS ; EFFECTOR NUCLEASES ; XENOPUS-TROPICALIS ; HORMONE GENE ; FOREIGN GENE ; ZEBRAFISH
WOS IDWOS:000429371100001
Funding OrganizationNational Natural Science Foundation of China(31721005 ; National Natural Science Foundation of China(31721005 ; Foundation of Beijing Municipal Science and Technology Project(Z161100004516003) ; Foundation of Beijing Municipal Science and Technology Project(Z161100004516003) ; Innovation Team of Sturgeon and Salmonid of Beijing Project(BAIC08-2018) ; Innovation Team of Sturgeon and Salmonid of Beijing Project(BAIC08-2018) ; Innovation Team of Sturgeon and Salmonid of Baafs Project(JNKST201611) ; Innovation Team of Sturgeon and Salmonid of Baafs Project(JNKST201611) ; Foundation of Beijing Academy of Agriculture and Forestry Sciences: Prospective study of new technology on sturgeon industry ; Foundation of Beijing Academy of Agriculture and Forestry Sciences: Prospective study of new technology on sturgeon industry ; 31325026) ; 31325026) ; National Natural Science Foundation of China(31721005 ; National Natural Science Foundation of China(31721005 ; Foundation of Beijing Municipal Science and Technology Project(Z161100004516003) ; Foundation of Beijing Municipal Science and Technology Project(Z161100004516003) ; Innovation Team of Sturgeon and Salmonid of Beijing Project(BAIC08-2018) ; Innovation Team of Sturgeon and Salmonid of Beijing Project(BAIC08-2018) ; Innovation Team of Sturgeon and Salmonid of Baafs Project(JNKST201611) ; Innovation Team of Sturgeon and Salmonid of Baafs Project(JNKST201611) ; Foundation of Beijing Academy of Agriculture and Forestry Sciences: Prospective study of new technology on sturgeon industry ; Foundation of Beijing Academy of Agriculture and Forestry Sciences: Prospective study of new technology on sturgeon industry ; 31325026) ; 31325026)
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/50937
Collection鱼类生物学及渔业生物技术研究中心
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Hubei, Peoples R China
2.Beijing Fisheries Res Inst, Beijing Key Lab Fishery Biotechnol, Beijing, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Qingdao, Peoples R China
Recommended Citation
GB/T 7714
Chen, Ji,Wang, Wei,Tian, Zhaohui,et al. Efficient Gene Transfer and Gene Editing in Sterlet (Acipenser ruthenus)[J]. FRONTIERS IN GENETICS,2018,9.
APA Chen, Ji.,Wang, Wei.,Tian, Zhaohui.,Dong, Ying.,Dong, Tian.,...&Hu, Wei.(2018).Efficient Gene Transfer and Gene Editing in Sterlet (Acipenser ruthenus).FRONTIERS IN GENETICS,9.
MLA Chen, Ji,et al."Efficient Gene Transfer and Gene Editing in Sterlet (Acipenser ruthenus)".FRONTIERS IN GENETICS 9(2018).
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