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题名: The Small Regulatory Antisense RNA PilR Affects Pilus Formation and Cell Motility by Negatively Regulating pilA11 in Synechocystis sp PCC6803
作者: Hu, Jinlu1; Zhan, Jiao2; Chen, Hui2, 3; He, Chenliu2; Cang, Huaixing1; Wang, Qiang2
关键词: Synechocystis sp PCC 6803 ; PilR ; pilA11 ; pili ; cell motility
刊名: FRONTIERS IN MICROBIOLOGY
发表日期: 2018-04-23
DOI: 10.3389/fmicb.2018.00786
卷: 9
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Microbiology
研究领域[WOS]: Microbiology
英文摘要: Pili are found on the surface of many bacteria and play important roles in cell motility, pathogenesis, biofilm formation, and sensing and reacting to environmental changes. Cell motility in the model cyanobacterium Synechocystis sp. PCC 6803 relies on expression of the putative pilA9-pilA10-pilA11-slr2018 operon. In this study, we identified the antisense RNA PilR encoded in the noncoding strand of the prepilin-encoding gene pilA11. Analysis of overexpressor [PilR(+)] and suppressor [PilR(-)] mutant strains revealed that PilR is a direct negative regulator of PilA11 protein. Although overexpression of PilR did not affect cell growth, it greatly reduced levels of pilA11 mRNA and protein and decreased both the thickness and number of pili, resulting in limited cell motility and small, distinct colonies. Suppression of PilR had the opposite effect. A hypothetical model on the regulation of pilA9-pilA10-pilA11-slr2018 operon expression by PilR was proposed. These results add a layer of complexity to the mechanisms controlling pilA11 gene expression and cell motility, and provide novel insights into how sRNA and the intergenic region secondary structures can work together to discoordinatly regulate target gene in an operon in cyanobacterium.
关键词[WOS]: CHAPERONE-USHER PATHWAY ; SP STRAIN PCC-6803 ; SP PCC 6803 ; ESCHERICHIA-COLI ; PROTEIN-KINASE ; IV PILI ; BIOGENESIS ; EXPRESSION ; BACTERIA ; GENES
语种: 英语
项目资助者: National Natural Science Foundation of China(31770128 ; State Key Laboratory of Freshwater Ecology and Biotechnology(Y11901-1-F01) ; Fundamental Research Funds for the Central Universities(3102017OQD042) ; China Postdoctoral Science Foundation(2017M610648) ; 31700107 ; 31700055)
WOS记录号: WOS:000430558200002
ISSN号: 1664-302X
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内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/50926
Appears in Collections:藻类生物学及应用研究中心_期刊论文

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作者单位: 1.Northwestern Polytech Univ, Sch Life Sci, Xian, Shaanxi, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan, Hubei, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan, Hubei, Peoples R China
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