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Molecular cloning, expression pattern of follistatin gene and association analysis with growth traits in bighead carp (Hypophthalmichthys nobilis)
Pang, Meixia1,2; Tong, Jingou1; Yu, Xiaomu1; Fu, Beide1; Zhou, Ying1,2
2018-04-01
Source PublicationCOMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
ISSN1096-4959
Volume218Pages:44-53
AbstractFollistatin (FST) is a single-chain gonadal protein involving in various biological effects. FST plays important roles in not only ovary development but also body growth, whereas myostatin (MSTN) negatively regulates muscle growth. In this study, FST gene in bighead carp (HynFST) was cloned and characterized. A 5797 bp genomic sequence of HynFST, consisting six exons and five introns were cloned. The full-length cDNA of HynFST (2134 bp) has an open reading fragment encoding a polypeptide of 349 amino acids. Sequence comparison and phylogenetic analysis confirmed that FSTs are conserved throughout the vertebrates and HynFST belongs to FST-1 isoform. Nine single nucleotide polymorphisms (SNPs) of the HynFST were identified and three of them (g.2443 T > C, g.2852 T > C and g.5483A > G) were significantly associated with four growth-related traits. The average body weight of those fish with the combined genotype (CC CC GG) was 12.15-22.63% higher than that of triplotype (TT TT AA) in two bighead carp populations. HynFST was expressed in most of the development stages and various tissues with highest level in ovary. The co-expression results for FST and MSTN in brain and muscle of divergent weight groups showed that FST may inhibit MSTN expression, thus enhancing growth in bighead carp. Our results suggest that FST has significant genetic effects on the regulation of early growth in bighead carp. This study would facilitate the elucidation of multiple functions of FST gene in fish and exploration of the potentials as a gene marker in selective breeding programs for growth of bighead carp.
SubtypeArticle
KeywordBighead Carp (Hypophthalmichthys Nobilis) Follistatin Myostatin Expression Snp Association Analysis Growth-related Traits
DOI10.1016/j.cbpb.2018.02.003
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationNational Science Foundation of China(31472268) ; National Science Foundation of China(31472268) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2016FBZ05) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2016FBZ05) ; National Science Foundation of China(31472268) ; National Science Foundation of China(31472268) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2016FBZ05) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2016FBZ05)
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Zoology
WOS SubjectBiochemistry & Molecular Biology ; Zoology
WOS KeywordFOLLICLE-STIMULATING-HORMONE ; TGF-BETA SUPERFAMILY ; SKELETAL-MUSCLE MASS ; GENOME DUPLICATION ; ACTIVIN-BINDING ; CYPRINUS-CARPIO ; MYOSTATIN GENE ; PROTEIN ; FISH ; MICE
WOS IDWOS:000429754600006
Funding OrganizationNational Science Foundation of China(31472268) ; National Science Foundation of China(31472268) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2016FBZ05) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2016FBZ05) ; National Science Foundation of China(31472268) ; National Science Foundation of China(31472268) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2016FBZ05) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2016FBZ05)
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/50920
Collection鱼类生物学及渔业生物技术研究中心
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Pang, Meixia,Tong, Jingou,Yu, Xiaomu,et al. Molecular cloning, expression pattern of follistatin gene and association analysis with growth traits in bighead carp (Hypophthalmichthys nobilis)[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2018,218:44-53.
APA Pang, Meixia,Tong, Jingou,Yu, Xiaomu,Fu, Beide,&Zhou, Ying.(2018).Molecular cloning, expression pattern of follistatin gene and association analysis with growth traits in bighead carp (Hypophthalmichthys nobilis).COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,218,44-53.
MLA Pang, Meixia,et al."Molecular cloning, expression pattern of follistatin gene and association analysis with growth traits in bighead carp (Hypophthalmichthys nobilis)".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 218(2018):44-53.
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