The improved growth performance and enhanced immune function by DL-methionyl-DL-methionine are associated with NF-kappa B and TOR signalling in intestine of juvenile grass carp (Ctenopharyngodon idella)
Su, Yue-Ning1; Wu, Pei1,2,3; Feng, Lin1,2,3; Jiang, Wei-Dan1,2,3; Jiang, Jun1; Zhang, Yong-An4; Figueiredo-Silva, Claudia5; Zhou, Xiao-Qiu1,2,3; Liu, Yang1,2,3
2018-03-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume74Pages:101-118
AbstractThe present study investigated the effects of dietary DL-methionyl-DL-methionine (Met-Met) on growth performance, intestinal immune function and the underlying signalling molecules in juvenile grass carp (Ctenopharyngodon idella). Fish were fed one DL-methionine (DL-Met) group (2.50 g/kg diet) and six graded levels of Met-Met groups (0, 0.79, 1.44, L84, 2.22 and 2.85 g/kg diet) for 10 weeks, and then challenged with Aeromonas hydrophila for 14 days. Results indicated that the optimal Met-Met supplementation: (1) increased fish growth performance, intestinal lysozyme (LZ) and acid phosphatase (ACP) activities, complement (C3 and C4) and immunoglobulin M (IgM) contents, up-regulated hepcidin, liver expressed antimicrobial peptide 2A (LEAP-2A), LEAP-2B, beta-defensin-1 and Mucin2 mRNA levels; (2) down-regulated tumour necrosis factor alpha (TNF-alpha), interferon gamma 2 (IFN-gamma 2), interleukin 1 beta (IL-1 beta), IL-8 [only in the distal intestine (DI)], IL-12p35, IL-12p40 and IL-15 (not IL-17D) mRNA levels partially related to the down-regulation of I kappa B kinase beta (IKK beta) and IKK gamma (rather than IKK alpha), nuclear factor kappa B (NF-kappa B) p65 and c-Rel (rather than NF-kappa B p52) mRNA levels and the up regulation of inhibitor of kappa B alpha (I kappa B alpha) mRNA levels; (3) up-regulated IL-4/13A, IL-4/13B, IL-6, IL-10, IL-11 and transforming growth factor (TGF)-beta 1 (not TGF-beta 2) mRNA levels partially associated with the target of rapamycin (TOR) signalling pathway [TOR/ribosomal protein S6 kinases 1 (S6K1), eIF4E-binding proteins (4E-BP)] in three intestinal segments of juvenile grass carp. These results suggest that Met-Met supplementation improves growth and intestinal immune function in fish. Furthermore, according to a positive effect, the optimal Met-Met supplementation was superior to the optimal DL-Met supplementation at improving the growth performance and enhancing the intestinal immune function in fish. Finally, based on percent weight gain (PWG), protection against enteritis morbidity and immune index (LZ activity), the optimal Met-Met supplementation for juvenile grass carp was estimated as 1.61, 1.64 and 1.68 g/kg diet, respectively, as the basal diet contains 8.03 g/kg total sulfur amino acids (TSAA) (4.26 g methionine/kg and 3.77 g cysteine/kg).
SubtypeArticle
KeywordDl-methionyl-dl-methionine Intestinal Immune Nf-kappa e Signalling Tor Signalling Grass Carp (Ctenopharyngodon Idella)
DOI10.1016/j.fsi.2017.12.051
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationNational Basic Research Program of China (973 Program)(2014CB138600) ; National Basic Research Program of China (973 Program)(2014CB138600) ; National Natural Science Foundation of China(31572632 ; National Natural Science Foundation of China(31572632 ; Outstanding Talents and Innovative Team of Agricultural Scientific Research (Ministry of Agriculture) ; Outstanding Talents and Innovative Team of Agricultural Scientific Research (Ministry of Agriculture) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; Specialized Research Fund for the Doctoral Program of Higher Education of China(20135103110001) ; Specialized Research Fund for the Doctoral Program of Higher Education of China(20135103110001) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Evonik Nutrition Care GmbH ; Evonik Nutrition Care GmbH ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Foundation of Sichuan Youth Science and Technology Innovation Research Team(2017TD0002) ; Foundation of Sichuan Youth Science and Technology Innovation Research Team(2017TD0002) ; Earmarked Fund for China Agriculture Research System(CARS-45) ; Earmarked Fund for China Agriculture Research System(CARS-45) ; 31672662) ; 31672662) ; 2013NC0045) ; 2013NC0045) ; National Basic Research Program of China (973 Program)(2014CB138600) ; National Basic Research Program of China (973 Program)(2014CB138600) ; National Natural Science Foundation of China(31572632 ; National Natural Science Foundation of China(31572632 ; Outstanding Talents and Innovative Team of Agricultural Scientific Research (Ministry of Agriculture) ; Outstanding Talents and Innovative Team of Agricultural Scientific Research (Ministry of Agriculture) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; Specialized Research Fund for the Doctoral Program of Higher Education of China(20135103110001) ; Specialized Research Fund for the Doctoral Program of Higher Education of China(20135103110001) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Evonik Nutrition Care GmbH ; Evonik Nutrition Care GmbH ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Foundation of Sichuan Youth Science and Technology Innovation Research Team(2017TD0002) ; Foundation of Sichuan Youth Science and Technology Innovation Research Team(2017TD0002) ; Earmarked Fund for China Agriculture Research System(CARS-45) ; Earmarked Fund for China Agriculture Research System(CARS-45) ; 31672662) ; 31672662) ; 2013NC0045) ; 2013NC0045)
Language英语
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS KeywordTIGHT JUNCTION PROTEINS ; BREAM MEGALOBRAMA-AMBLYCEPHALA ; DIETARY LYSINE REQUIREMENT ; PHYSICAL BARRIER FUNCTION ; SCOPHTHALMUS-MAXIMUS L. ; SALMON SALMO-SALAR ; KINASE-C-ZETA ; CYPRINUS-CARPIO ; GENE-EXPRESSION ; AEROMONAS-HYDROPHILA
WOS IDWOS:000427344200013
Funding OrganizationNational Basic Research Program of China (973 Program)(2014CB138600) ; National Basic Research Program of China (973 Program)(2014CB138600) ; National Natural Science Foundation of China(31572632 ; National Natural Science Foundation of China(31572632 ; Outstanding Talents and Innovative Team of Agricultural Scientific Research (Ministry of Agriculture) ; Outstanding Talents and Innovative Team of Agricultural Scientific Research (Ministry of Agriculture) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; Specialized Research Fund for the Doctoral Program of Higher Education of China(20135103110001) ; Specialized Research Fund for the Doctoral Program of Higher Education of China(20135103110001) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Evonik Nutrition Care GmbH ; Evonik Nutrition Care GmbH ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Foundation of Sichuan Youth Science and Technology Innovation Research Team(2017TD0002) ; Foundation of Sichuan Youth Science and Technology Innovation Research Team(2017TD0002) ; Earmarked Fund for China Agriculture Research System(CARS-45) ; Earmarked Fund for China Agriculture Research System(CARS-45) ; 31672662) ; 31672662) ; 2013NC0045) ; 2013NC0045) ; National Basic Research Program of China (973 Program)(2014CB138600) ; National Basic Research Program of China (973 Program)(2014CB138600) ; National Natural Science Foundation of China(31572632 ; National Natural Science Foundation of China(31572632 ; Outstanding Talents and Innovative Team of Agricultural Scientific Research (Ministry of Agriculture) ; Outstanding Talents and Innovative Team of Agricultural Scientific Research (Ministry of Agriculture) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; Specialized Research Fund for the Doctoral Program of Higher Education of China(20135103110001) ; Specialized Research Fund for the Doctoral Program of Higher Education of China(20135103110001) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Evonik Nutrition Care GmbH ; Evonik Nutrition Care GmbH ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Foundation of Sichuan Youth Science and Technology Innovation Research Team(2017TD0002) ; Foundation of Sichuan Youth Science and Technology Innovation Research Team(2017TD0002) ; Earmarked Fund for China Agriculture Research System(CARS-45) ; Earmarked Fund for China Agriculture Research System(CARS-45) ; 31672662) ; 31672662) ; 2013NC0045) ; 2013NC0045)
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/50915
Collection藻类生物学及应用研究中心_水生生物分子与细胞生物学研究中心
Affiliation1.Sichuan Agr Univ, Anim Nutr Inst, Chengdu 611130, Sichuan, Peoples R China
2.Sichuan Agr Univ, Fish Nutr & Safety Prod Univ Key Lab Sichuan Prov, Chengdu 611130, Sichuan, Peoples R China
3.Sichuan Agr Univ, Minist Educ, Key Lab Anim Dis Resistance Nutr China, Chengdu 611130, Sichuan, Peoples R China
4.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
5.Evon Nutr & Care GmbH, NC, 10-B531,Postfach 1345,Rodenbacher Chausse 4, D-63404 Hanau, Germany
Recommended Citation
GB/T 7714
Su, Yue-Ning,Wu, Pei,Feng, Lin,et al. The improved growth performance and enhanced immune function by DL-methionyl-DL-methionine are associated with NF-kappa B and TOR signalling in intestine of juvenile grass carp (Ctenopharyngodon idella)[J]. FISH & SHELLFISH IMMUNOLOGY,2018,74:101-118.
APA Su, Yue-Ning.,Wu, Pei.,Feng, Lin.,Jiang, Wei-Dan.,Jiang, Jun.,...&Liu, Yang.(2018).The improved growth performance and enhanced immune function by DL-methionyl-DL-methionine are associated with NF-kappa B and TOR signalling in intestine of juvenile grass carp (Ctenopharyngodon idella).FISH & SHELLFISH IMMUNOLOGY,74,101-118.
MLA Su, Yue-Ning,et al."The improved growth performance and enhanced immune function by DL-methionyl-DL-methionine are associated with NF-kappa B and TOR signalling in intestine of juvenile grass carp (Ctenopharyngodon idella)".FISH & SHELLFISH IMMUNOLOGY 74(2018):101-118.
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