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题名: Dietary myo-inositol deficiency decreased intestinal immune function related to NF-kappa B and TOR signaling in the intestine of young grass carp (Ctenopharyngodon idella)
作者: Li, Shuang-An1; Jiang, Wei-Dan1, 2, 3; Feng, Lin1, 2, 3; Liu, Yang1, 2, 3; Wu, Pei1, 2, 3; Jiang, Jun1, 2, 3; Kuang, Sheng-Yao4; Tang, Ling4; Tang, Wu-Neng4; Zhang, Yong-An5; Yang, Juan6; Tang, Xu7; Shi, He-Qun8; Zhou, Xiao-Qiu1, 2, 3
关键词: Myo-inositol ; Grass carp (Ctenopharyngodon idella) ; Intestine ; Immune function
刊名: FISH & SHELLFISH IMMUNOLOGY
发表日期: 2018-05-01
DOI: 10.1016/j.fsi.2018.03.017
卷: 76, 页:333-346
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
研究领域[WOS]: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
英文摘要: In this study, we investigated the effects of dietary myo-inositol on the intestinal immune barrier function and related signaling pathway in young grass carp (Ctenopharyngodon idella). A total of 540 young grass carp (221.33 +/- 0.84 g) were fed six diets containing graded levels of myo-inositol (27.0, 137.9, 286.8, 438.6, 587.7 and 737.3 mg/kg) for 10 weeks. After the growth trial, fish were challenged with Aeromonas hydrophila. The results indicated that compared with the optimal dietary myo-inositol level, myo-inositol deficiency (27.0 mg/kg diet): (1) decreased lysozyme (LZ) and acid phosphatase (ACP) activities, as well as complement 3 (C3), C4 and immunoglobulin M (IgM) contents in the proximal intestine (PI), middle intestine (MI) and distal intestine (DI) of young grass carp (P < 0.05). (2) down-regulated the mRNA levels of anti-microbial substance: liver expressed antimicrobial peptide (LEAP) 2A, LEAP-2B, hepcidin, beta-defensin-1 and mucin2 in the PI, MI and DI of young grass carp (P < 0.05). (3) up-regulated pro-inflammatory cytokines [IL-1 beta (not in DI), TNF-alpha and IL-8], nuclear factor kappa B P65 (not NF-kappa B P52), c-Rel, I kappa B kinasea (IKK alpha), IKK beta and IKK gamma mRNA levels in the PI, MI and DI of young grass carp (P < 0.05); and down-regulated pro-inflammatory cytokines IL-15 (not in DI) and inhibitor of kappa B alpha (I kappa B alpha) mRNA levels (P < 0.05). (4) down-regulated the mRNA levels of anti-inflammatory cytokines [IL-10 (not in DI), IL-11, IL-4/13B (not IL-4/13A), TGF-beta 1 and TGF-beta 2], target of rapamycin (TOR), eIF4E-binding proteins 1 (4E-BP1) and ribosomal protein S6 kinase 1 (S6k1) in the PI, MI and DI of young grass carp (P < 0.05). All data indicated that myo-inositol deficiency could decrease fish intestine immunity and cause inflammation under infection of A. hydrophila. Finally, the optimal dietary myo-inositol levels for the ACP and LZ activities in the DI were estimated to be 415.1 and 296.9 mg/kg diet, respectively.
关键词[WOS]: PHYSICAL BARRIER FUNCTION ; TIGHT JUNCTION PROTEINS ; MESSENGER-RNA EXPRESSION ; FED GRADED-LEVELS ; GROWTH-PERFORMANCE ; DISEASE RESISTANCE ; GENE-EXPRESSION ; TELEOST FISH ; AEROMONAS-HYDROPHILA ; NONSPECIFIC IMMUNITY
语种: 英语
项目资助者: National Natural Science Foundation of China(31001116 ; National Basic Research Program of China (973 Program)(2014CB138600) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; Outstanding Talents and Innovative Team of Agricultural Scientific Research (Ministry of Agriculture) ; Specialized Research Fund for the Doctoral Program of Higher Education of China(20135103110001) ; Earmarked Fund for China Agriculture Research System(CARS-45) ; Key Fund Project of Sichuan Provincial Department of Education(14ZA0007) ; Science and Technology Support Programme of Sichuan Province of China(2014NZ0003) ; Foundation of Sichuan Youth Science and Technology Innovation Research Team(2017TD0002) ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2013NC0045) ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; 31272674 ; 31302197)
WOS记录号: WOS:000430888400040
ISSN号: 1050-4648
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内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/50903
Appears in Collections:水生生物分子与细胞生物学研究中心_期刊论文

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作者单位: 1.Sichuan Agr Univ, Anim Nutr Inst, Chengdu 611130, Sichuan, Peoples R China
2.Sichuan Agr Univ, Fish Nutr & Safety Prod Univ Key Lab Sichuan Prov, Chengdu 611130, Sichuan, Peoples R China
3.Sichuan Agr Univ, China Minist Educ, Key Lab Anim Dis Resistance Nutr, Chengdu 611130, Sichuan, Peoples R China
4.Sichuan Acad Anim Sci, Anim Nutr Inst, Chengdu 610066, Sichuan, Peoples R China
5.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
6.Tongwei Co Ltd, Enterprise Technol Ctr, Chengdu 610041, Sichuan, Peoples R China
7.Chengdu Mytech Biotech Co Ltd, Chengdu 610222, Sichuan, Peoples R China
8.Guangzhou Cohoo Biotech Res & Dev Ctr, Guangzhou 510663, Guangdong, Peoples R China
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