IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心
Molecular cloning, expression analysis and localization pattern of the MST family in grass carp (Ctenopharyngodon idella)
Chu, Pengfei1,2; He, Libo1; Xiong, Lv1,2; Luo, Lifei1,2; Huang, Rong1; Liao, Lanjie1; Li, Yongming1; Zhu, Zuoyan1; Wang, Yaping1
2018-05-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume76Pages:316-323
AbstractThe mammalian sterile 20-like (MST) family, which belongs to the serine/threonine protein kinase superfamily, has five members that can be found in mammals: STK3 (also called MST2), STK4 (MST1), STK24 (MST3), STK25 (YSK1 or SOK1), and STK26 (MST4). The MST kinases have key roles in apoptosis, immune regulation, inflammatory responses, cancer, and cell proliferation in mammals, whereas the roles and transcriptional regulatory mechanism of these kinases in teleost fish are still unclear. In this study, four STK genes (CiSTK3, CiSTK24, CiSTK25, and CiSTK26) were cloned and analyzed in grass carp (Ctenopharyngodon idella). All four STK genes were broadly expressed in the examined tissues, while their relative expression levels differed. In addition, after exposure to the grass carp reovirus, mRNA expression levels of the four STK genes were altered to different levels in the immune organs, and the levels were dramatically altered in the blood. Subcellular localization indicated that all four STK proteins were localized in the cytoplasm of transfected cells. Moreover, bimolecular fluorescence complementation analysis revealed that mouse protein-25 could interact with CiSTK3, CiSTK24, CiSTK25, and CiSTK26 independently in grass carp. Thus, our findings provide new insights for understanding the functions of the MST family in teleosts.
SubtypeArticle
KeywordMammalian Sterile 20-like Immune Regulation Subcellular Localization Bimolecular Fluorescence Complementation
DOI10.1016/j.fsi.2018.03.021
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationNational Natural Science Foundation of China(31721005) ; National Natural Science Foundation of China(31721005) ; Hubei Natural Science Foundation of China(2015CFA007) ; Hubei Natural Science Foundation of China(2015CFA007) ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science(XDA08030203) ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science(XDA08030203) ; National Natural Science Foundation of China(31721005) ; National Natural Science Foundation of China(31721005) ; Hubei Natural Science Foundation of China(2015CFA007) ; Hubei Natural Science Foundation of China(2015CFA007) ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science(XDA08030203) ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science(XDA08030203)
Language英语
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS KeywordPROVIDES INSIGHTS ; KINASE MST1 ; APOPTOSIS ; PROTEIN ; MO25 ; ACTIVATION ; PHOSPHORYLATION ; REOVIRUS ; COMPLEX ; MECHANISM
WOS IDWOS:000430888400038
Funding OrganizationNational Natural Science Foundation of China(31721005) ; National Natural Science Foundation of China(31721005) ; Hubei Natural Science Foundation of China(2015CFA007) ; Hubei Natural Science Foundation of China(2015CFA007) ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science(XDA08030203) ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science(XDA08030203) ; National Natural Science Foundation of China(31721005) ; National Natural Science Foundation of China(31721005) ; Hubei Natural Science Foundation of China(2015CFA007) ; Hubei Natural Science Foundation of China(2015CFA007) ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science(XDA08030203) ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Science(XDA08030203)
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/50898
Collection鱼类生物学及渔业生物技术研究中心
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Chu, Pengfei,He, Libo,Xiong, Lv,et al. Molecular cloning, expression analysis and localization pattern of the MST family in grass carp (Ctenopharyngodon idella)[J]. FISH & SHELLFISH IMMUNOLOGY,2018,76:316-323.
APA Chu, Pengfei.,He, Libo.,Xiong, Lv.,Luo, Lifei.,Huang, Rong.,...&Wang, Yaping.(2018).Molecular cloning, expression analysis and localization pattern of the MST family in grass carp (Ctenopharyngodon idella).FISH & SHELLFISH IMMUNOLOGY,76,316-323.
MLA Chu, Pengfei,et al."Molecular cloning, expression analysis and localization pattern of the MST family in grass carp (Ctenopharyngodon idella)".FISH & SHELLFISH IMMUNOLOGY 76(2018):316-323.
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