IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心
An miR-200 Cluster on Chromosome 23 Regulates Sperm Motility in Zebrafish
Xiong, Shuting1; Ma, Wenge1; Jing, Jing1; Zhang, Jin1; Dan, Cheng1; Gui, Jian-Fang1,2; Mei, Jie1
2018-05-01
Source PublicationENDOCRINOLOGY
ISSN0013-7227
Volume159Issue:5Pages:1982-1991
AbstractBesides its well-documented roles in cell proliferation, apoptosis, and carcinogenesis, the function of the p53-microRNA axis has been recently revealed in the reproductive system. Recent studies indicated that miR-200 family members are dysregulated in nonobstructive azoospermia patients, whereas their functions remain poorly documented. The aim of this study was to investigate the function of the miR-200 family on zebrafish testis development and sperm activity. There was no substantial difference in testis morphology and histology between wild-type (WT) and knockout zebrafish with deletion of miR-200 cluster on chromosome 6 (chr6-miR-200-KO) or on chromosome 23 (chr23-miR-200-KO). Interestingly, compared with WT zebrafish, the chr6-miR-200-KO zebrafish had no difference on sperm motility, whereas chr23-miR-200-KO zebrafish showed significantly improved sperm motility. Consistently, ectopic expression of miR-429a, miR-200a, and miR-200b, which are located in the miR-200 cluster on chromosome 23, significantly reduced motility traits of sperm. Several sperm motility-related genes, such as amh, wt1a, and srd5a2b have been confirmed as direct targets of miR-200s on chr23. 17a-ethynylestradiol (EE2) exposure resulted in upregulated expression of p53 and miR-429a in testis and impairment of sperm motility. Strikingly, in p53 mutant zebrafish testis, the expression levels of miR-200s on chr23 were significantly reduced and accompanied by a stimulation of sperm motility. Moreover, the upregulation of miR-429a associated with EE2 treatment was abolished in testis with p53 mutation. And the impairment of sperm activity by EE2 treatment was also eliminated when p53 was mutated. Together, our results reveal that miR-200 cluster on chromosome 23 controls sperm motility in a p53-dependent manner.
SubtypeArticle
DOI10.1210/en.2018-00015
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationFundamental Research Funds for the Central Universities(529020900202949) ; Fundamental Research Funds for the Central Universities(529020900202949) ; National Natural Science Foundation of China(31672635) ; National Natural Science Foundation of China(31672635) ; Key Program of Frontier Sciences of the Chinese Academy of Sciences(QYZDY-SSW-SMC025) ; Key Program of Frontier Sciences of the Chinese Academy of Sciences(QYZDY-SSW-SMC025) ; Fundamental Research Funds for the Central Universities(529020900202949) ; Fundamental Research Funds for the Central Universities(529020900202949) ; National Natural Science Foundation of China(31672635) ; National Natural Science Foundation of China(31672635) ; Key Program of Frontier Sciences of the Chinese Academy of Sciences(QYZDY-SSW-SMC025) ; Key Program of Frontier Sciences of the Chinese Academy of Sciences(QYZDY-SSW-SMC025)
Language英语
WOS Research AreaEndocrinology & Metabolism
WOS SubjectEndocrinology & Metabolism
WOS KeywordANTI-MULLERIAN HORMONE ; SPERMATOGONIAL STEM-CELLS ; MALE-INFERTILITY ; SEMINAL PLASMA ; SEX DETERMINATION ; QUALITY-CONTROL ; ANALYSIS CASA ; SELF-RENEWAL ; MICRORNA ; SPERMATOGENESIS
WOS IDWOS:000432685100007
Funding OrganizationFundamental Research Funds for the Central Universities(529020900202949) ; Fundamental Research Funds for the Central Universities(529020900202949) ; National Natural Science Foundation of China(31672635) ; National Natural Science Foundation of China(31672635) ; Key Program of Frontier Sciences of the Chinese Academy of Sciences(QYZDY-SSW-SMC025) ; Key Program of Frontier Sciences of the Chinese Academy of Sciences(QYZDY-SSW-SMC025) ; Fundamental Research Funds for the Central Universities(529020900202949) ; Fundamental Research Funds for the Central Universities(529020900202949) ; National Natural Science Foundation of China(31672635) ; National Natural Science Foundation of China(31672635) ; Key Program of Frontier Sciences of the Chinese Academy of Sciences(QYZDY-SSW-SMC025) ; Key Program of Frontier Sciences of the Chinese Academy of Sciences(QYZDY-SSW-SMC025)
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/50891
Collection鱼类生物学及渔业生物技术研究中心
Affiliation1.Huazhong Agr Univ, Coll Fisheries, Key Lab Freshwater Anim Breeding, Minist Agr, Wuhan 430070, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Xiong, Shuting,Ma, Wenge,Jing, Jing,et al. An miR-200 Cluster on Chromosome 23 Regulates Sperm Motility in Zebrafish[J]. ENDOCRINOLOGY,2018,159(5):1982-1991.
APA Xiong, Shuting.,Ma, Wenge.,Jing, Jing.,Zhang, Jin.,Dan, Cheng.,...&Mei, Jie.(2018).An miR-200 Cluster on Chromosome 23 Regulates Sperm Motility in Zebrafish.ENDOCRINOLOGY,159(5),1982-1991.
MLA Xiong, Shuting,et al."An miR-200 Cluster on Chromosome 23 Regulates Sperm Motility in Zebrafish".ENDOCRINOLOGY 159.5(2018):1982-1991.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Xiong, Shuting]'s Articles
[Ma, Wenge]'s Articles
[Jing, Jing]'s Articles
Baidu academic
Similar articles in Baidu academic
[Xiong, Shuting]'s Articles
[Ma, Wenge]'s Articles
[Jing, Jing]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Xiong, Shuting]'s Articles
[Ma, Wenge]'s Articles
[Jing, Jing]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.