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题名: Characterization of Ferredoxin-Dependent Biliverdin Reductase PCYA1 Reveals the Dual Function in Retrograde Bilin Biosynthesis and Interaction With Light-Dependent Protochlorophyllide Oxidoreductase LPOR in Chlamydomonas reinhardtii
作者: Zhang, Weiqing1; Zhong, Huan1; Lu, Hui1; Zhang, Yuxiang1; Deng, Xuan2; Huang, Kaiyao2; Duanmu, Deqiang1
关键词: PCYA1 ; bilin ; POR ; chlorophyll biosynthesis ; algae ; photoacclimation
刊名: FRONTIERS IN PLANT SCIENCE
发表日期: 2018-05-23
DOI: 10.3389/fpls.2018.00676
卷: 9
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Plant Sciences
研究领域[WOS]: Plant Sciences
英文摘要: Bilins are linear tetrapyrroles commonly used as chromophores of phycobiliproteins and phytochromes for light-harvesting or light-sensing in photosynthetic organisms. Many eukaryotic algae lack both phycobiliproteins and phytochromes, but retain the bilin biosynthetic enzymes including heme oxygenase (HO/HMOX) and ferredoxin-dependent biliverdin reductase (FDBR). Previous studies on Chlamydomonas reinhardtii heme oxygenase mutant (hmox1) have shown that bilins are not only essential retrograde signals to mitigate oxidative stress during diurnal dark-to-light transitions, they are also required for chlorophyll accumulation and maintenance of a functional photosynthetic apparatus in the light. However, the underlying mechanism of bilin-mediated regulation of chlorophyll biosynthesis is unclear. In this study, Chlamydomonas phycocyanobilin:ferredoxin oxidoreductase PCYA1 FDBR domain was found to specifically interact with the rate-limiting chlorophyll biosynthetic enzyme LPOR (light-dependent protochlorophyllide oxidoreductase). PCYA1 is partially associated with chloroplast envelope membrane, consistent with the observed export of bilin from chloroplast to cytosol by cytosolic expression of a bilin-binding reporter protein in Chlamydomonas. Both the pcya1-1 mutant with the carboxyl-terminal extension of PCYA1 eliminated and efficient knockdown of PCYA1 expression by artificial microRNA exhibited no significant impact on algal phototrophic growth and photosynthetic proteins accumulation, indicating that the conserved FDBR domain is sufficient and minimally required for bilin biosynthesis and functioning. Taken together, these studies provide novel insights into the regulatory role of PCYA1 in chlorophyll biosynthesis via interaction with key Chl biosynthetic enzyme.
关键词[WOS]: TETRAPYRROLE BIOSYNTHESIS ; GENE-EXPRESSION ; CHLOROPHYLL BIOSYNTHESIS ; PHYTOCHROMOBILIN SYNTHASE ; MG-PROTOPORPHYRINIX ; ALGAL PHYTOCHROMES ; OXIDATIVE STRESS ; PLASTID SIGNALS ; GREEN-ALGA ; PLANTS
语种: 英语
项目资助者: National Natural Science Foundation of China(31570233) ; Huazhong Agricultural University Scientific and Technological Self-innovation Foundation(2014RC018) ; Fundamental Research Funds for the Central Universities(2662015PY171)
WOS记录号: WOS:000432772000001
ISSN号: 1664-462X
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内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/50888
Appears in Collections:藻类生物学及应用研究中心_期刊论文

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作者单位: 1.Huazhong Agr Univ, Coll Life Sci & Technol, State Key Lab Agr Microbiol, Wuhan, Hubei, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan, Hubei, Peoples R China
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