中国科学院水生生物研究所机构知识库
Advanced  
IHB OpenIR  > 淡水生态学研究中心  > 期刊论文
题名: N-acetylcysteine protects against microcystin-LR-induced endoplasmic reticulum stress and germ cell apoptosis in zebrafish testes
作者: Zhao, Sujuan1; Liu, Ying2; Wang, Fang1; Xu, Dexiang1; Xie, Ping3
关键词: Microcystin ; Endoplasmic reticulum stress ; Apoptosis ; N-acetylcysteine (NAC) ; Zebrafish testes
刊名: CHEMOSPHERE
发表日期: 2018-08-01
DOI: 10.1016/j.chemosphere.2018.04.020
卷: 204, 页:463-473
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Environmental Sciences
研究领域[WOS]: Environmental Sciences & Ecology
英文摘要: Previous studies have shown that microcystin-LR (MCLR) is a reproductive toxicant that induces germ cell apoptosis in the testes, but the underlying mechanisms have not been well understood. In this study, we investigated that MCLR induces germ cell apoptosis is through activation of endoplasmic reticulum (ER) stress and N-acetylcysteine (NAC), an antioxidant could protect against germ cell apoptosis by inhibiting the ER stress. Healthy male zebrafish were intraperitoneally injected with NAC (500 nM), beginning at 2 h before different doses of MCLR (0, 50,100, 200 mu g/kg). As expected, acute MCLR exposure resulted in oxidative stress and germ cell apoptosis in zebrafish testes. Further analysis showed that NAC significantly alleviated MCLR-induced testicular germ cell apoptosis and inhibited the caspase-dependent apoptotic proteins. Meanwhile H&E staining showed that NAC could rescue testicular damage induced by MCLR. Moreover, MCLR induced activation of ER stress which consequently triggered apoptosis in zebrafish testes. Interestingly, NAC was effective in improving the total antioxidant capacity (T-AOC) level and activity of antioxidant enzymes in NAC pretreated groups. NAC significantly attenuated MCLR-induced upregulation of GRP78 in testes. In addition, NAC significantly attenuated MCLR-triggered testicular eiF2s1 and MAPK8 activation, indicating that NAC counteracts MCLR-induced unfolded protein response (UPR) in testes. Taken together, the results observed in this study suggested that ER stress plays a critical role in germ cell apoptosis exposed to MCLR and NAC could protect against apoptosis via inhibiting ER stress in zebrafish testes. (C) 2018 Elsevier Ltd. All rights reserved.
关键词[WOS]: NIGROMACULATA IN-VIVO ; OXIDATIVE STRESS ; ANTIOXIDANT RESPONSES ; ORYZIAS-LATIPES ; DIVERSE ORGANS ; ANIMAL-CELLS ; ER ; TOXICITY ; LIVER ; EXPOSURE
语种: 英语
项目资助者: National Natural Science Foundation of China(31400455) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2017FB16)
WOS记录号: WOS:000432767300054
ISSN号: 0045-6535
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/50876
Appears in Collections:淡水生态学研究中心_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Anhui Med Univ, Sch Publ Hlth, Hefei 230032, Anhui, Peoples R China
2.Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab High Magnet Field & Ion Beam Phys Biol, Hefei 230031, Anhui, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Hubei, Peoples R China
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Zhao, Sujuan]'s Articles
[Liu, Ying]'s Articles
[Wang, Fang]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Zhao, Sujuan]‘s Articles
[Liu, Ying]‘s Articles
[Wang, Fang]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2018  中国科学院水生生物研究所 - Feedback
Powered by CSpace