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题名: Characterization of Microcystis (Cyanobacteria) Genotypes Based on the Internal Transcribed Spacer Region of rRNA by Next-Generation Sequencing
作者: Huo, Da1, 2; Chen, Youxin2; Zheng, Tao1; Liu, Xiang2; Zhang, Xinyue1; Yu, Gongliang2; Qiao, Zhiyi1; Li, Renhui2
关键词: cyanobacteria ; Yuqiao reservoir ; Haihe River ; high throughput sequencing ; ITS region
刊名: FRONTIERS IN MICROBIOLOGY
发表日期: 2018-05-15
DOI: 10.3389/fmicb.2018.00971
卷: 9
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Microbiology
研究领域[WOS]: Microbiology
英文摘要: Microcystis is one of the most common and dominant bloom-forming cyanobacteria in freshwater worldwide. The method for genotype detection based on traditional molecular cloning is expensive and time consuming and generates a limited number of sequences. In this study, a high-throughput sequencing (HTS) method was developed to detect the internal transcribed spacer (ITS) regions between 16S and 23S rRNA region of Microcystis populations along a typical water system in Yuqiao Reservoir-Haihe River in Tianjin, northern China. A total of 629,341 reads were obtained and clustered into 2005 operational taxonomic units (OTUs). Analysis of alpha diversity indices showed that the Haihe River is more diverse than Yuqiao Reservoir. In general, the two water areas exhibit a clear differentiation pattern in OTU abundance, sharing genotypes from a small part of Yuqiao Reservoir with those in the Haihe River. Phylogenetic analysis further indicated the possible flexible evolution of Microcystis genotypes occurring in the research areas. This study provides the first exhaustive description of HTS method for detection of ITS region to evaluate Microcystis intra-species diversity and relationship.
关键词[WOS]: REAL-TIME PCR ; GENETIC DIVERSITY ; LAKE TAIHU ; TOXIC CYANOBACTERIUM ; SPATIOTEMPORAL CHANGES ; ENVIRONMENTAL-FACTORS ; BLOOM ; POPULATIONS ; CHINA ; AERUGINOSA
语种: 英语
项目资助者: National Science Foundation of China(NSFC51779247) ; International Science and Technology Cooperation Program of China(2013DFA71340) ; Tianjin Agricultural University Key Laboratory of Aqua-ecology platform project
WOS记录号: WOS:000432290000001
ISSN号: 1664-302X
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内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/50871
Appears in Collections:藻类生物学及应用研究中心_期刊论文

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作者单位: 1.Tianjin Agr Univ, Dept Fisheries Sci, Natl Demonstrat Ctr Expt Aquaecol & Aquaculture E, Tianjin, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan, Hubei, Peoples R China
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