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题名: An enzymatic mechanism for balancing the stoichiometry of nitrogen and phosphorus in a shallow Chinese eutrophic lake
作者: Xiao, Jian1, 2; Wang, Si-Yang1, 2; Zhou, Zi-Jun1, 2; Zhang, Yao1, 2; Song, Chun-Lei1; Zhou, Yi-Yong1; Cao, Xiu-Yun1
关键词: Stoichiometry of nitrogen and phosphorus ; Nitrogen deficiency ; Alkaline phosphatase ; Leucine aminopeptidase ; Michaelis-Menten kinetics ; Eutrophication
刊名: SCIENCE OF THE TOTAL ENVIRONMENT
发表日期: 2018-07-15
DOI: 10.1016/j.scitotenv.2018.02.297
卷: 630, 页:1071-1077
收录类别: SCI ; ISTP
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Environmental Sciences
研究领域[WOS]: Environmental Sciences & Ecology
英文摘要: The over-enrichment of lake waters with nitrogen (N) and phosphorus (P) has become a serious environmental problem, but modes of change in stoichiometry and enzymatic regeneration along trophic gradients are largely unknown. Seasonal variations in the kinetics of extracellular aminopeptidase (LAP) and alkaline phosphatase (AP), together with the composition of phytoplankton and concentrations of N and P, were examined from Jun 2013 to September 2014 in a Chinese shallow lake in which two basins had contrasting trophic states. The turbid basin had a significantly higher concentration of chlorophyll a and lower ratios of N to P. In parallel, the turnover time of organic N mediated by LAP (T-LAP) was significantly shorter, and its maximum velocity (V-max) was significantly higher compared to those in the clear basin. Considering data from both basins, there were linear decreases in N/P and the ratios between dissolved inorganic N and total N with an increasing trophic state index, coupled with a significantly positive relationship between N/P and LAPT. Additionally, with decreasing TN/TP, the Michaelis constant (K-m) of the AP increased linearly, reducing the efficiency of P regeneration. In contrast, the Km value of LAP decreased, and V-max increased, which enhanced Nmineralization by simultaneously increasing the reaction velocity and improving the affinity for substrate. Additionally, the K-m value of LAP was significantly related to that of AP and the ammonium concentration. Thus, substrate affinity acted as a key factor modifying the pathways of enzymatic degradation of organic N and P according to their stoichiometry in the water column. Phytoplankton composition was directly linked to LAPT. Overall, this study seemed to be the first to connect a stoichiometric shift of N and P with kinetics of extracellular enzymes responsible for their regeneration along trophic gradients, presenting an additional pathway to overcome nitrogen deficiency in eutrophic lakes. (C) 2018 Elsevier B.V. All rights reserved.
关键词[WOS]: AMINOPEPTIDASE ACTIVITY ; ALKALINE-PHOSPHATASE ; NUTRIENT LIMITATION ; BACTERIAL-ACTIVITY ; TROPHIC GRADIENT ; ORGANIC-MATTER ; SEDIMENT ; KINETICS ; PHYTOPLANKTON ; RATIOS
语种: 英语
项目资助者: National Natural Science Foundation of China(41573110 ; Major Science and Technology Program for Water Pollution Control and Treatment(2017ZX07603) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2016FBZ07) ; 41230748 ; 41173081)
WOS记录号: WOS:000432467700105
ISSN号: 0048-9697
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/50870
Appears in Collections:水环境工程研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Key Lab Algal Biol, 7 Donghu South Rd, Wuhan 430072, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
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