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题名: Three Substrains of the Cyanobacterium Anabaena sp Strain PCC 7120 Display Divergence in Genomic Sequences and hetC Function
作者: Wang, Yali1, 2; Gao, Yuan1; Gao, Hong1, 2; Zhang, Cheng-Cai2; Xu, Xudong1, 2
关键词: Anabaena sp ; strain PCC 7120 ; microevolution ; heterocyst differentiation
刊名: JOURNAL OF BACTERIOLOGY
发表日期: 2018-07-01
DOI: 10.1128/JB.00076-18
卷: 200, 期:13
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Microbiology
研究领域[WOS]: Microbiology
英文摘要: Anabaena sp. strain PCC 7120 is a model strain for molecular studies of cell differentiation and patterning in heterocyst-forming cyanobacteria. Subtle differences in heterocyst development have been noticed in different laboratories working on the same organism. In this study, 360 mutations, including single nucleotide polymorphisms (SNPs), small insertion/deletions (indels; 1 to 3 bp), fragment deletions, and transpositions, were identified in the genomes of three substrains. Heterogeneous/heterozygous bases were also identified due to the polyploidy nature of the genome and the multicellular morphology but could be completely segregated when plated after filament fragmentation by somcation. hetC is a gene upregulated in developing cells during heterocyst formation in Anabaena sp. strain PCC 7120 and found in approximately half of other heterocyst-forming cyanobacteria. Inactivation of hetC in 3 substrains of Anabaena sp. PCC 7120 led to different phenotypes: the formation of heterocysts, differentiating cells that keep dividing, or the presence of both heterocysts and dividing differentiating cells. The expression of P-het7-gfp in these hetC mutants also showed different patterns of green fluorescent protein (GFP) fluorescence. Thus, the function of hetC is influenced by the genomic background and epistasis and constitutes an example of evolution under way.
关键词[WOS]: HETEROCYST PATTERN-FORMATION ; INTERCELLULAR TRANSFER ; INSERTION SEQUENCES ; RESPONSE REGULATOR ; ESCHERICHIA-COLI ; SP PCC-7120 ; DIFFERENTIATION ; GENE ; EXPRESSION ; EVOLUTION
语种: 英语
项目资助者: National Natural Science Foundation of China(31270132 ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS(2016FBZ09) ; 31770044)
WOS记录号: WOS:000434807300004
ISSN号: 0021-9193
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内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/50866
Appears in Collections:藻类生物学及应用研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Hubei, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan, Hubei, Peoples R China
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