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Three Substrains of the Cyanobacterium Anabaena sp Strain PCC 7120 Display Divergence in Genomic Sequences and hetC Function
Wang, Yali1,2; Gao, Yuan1; Gao, Hong1,2; Zhang, Cheng-Cai2; Xu, Xudong1,2
2018-07-01
Source PublicationJOURNAL OF BACTERIOLOGY
ISSN0021-9193
Volume200Issue:13
AbstractAnabaena sp. strain PCC 7120 is a model strain for molecular studies of cell differentiation and patterning in heterocyst-forming cyanobacteria. Subtle differences in heterocyst development have been noticed in different laboratories working on the same organism. In this study, 360 mutations, including single nucleotide polymorphisms (SNPs), small insertion/deletions (indels; 1 to 3 bp), fragment deletions, and transpositions, were identified in the genomes of three substrains. Heterogeneous/heterozygous bases were also identified due to the polyploidy nature of the genome and the multicellular morphology but could be completely segregated when plated after filament fragmentation by somcation. hetC is a gene upregulated in developing cells during heterocyst formation in Anabaena sp. strain PCC 7120 and found in approximately half of other heterocyst-forming cyanobacteria. Inactivation of hetC in 3 substrains of Anabaena sp. PCC 7120 led to different phenotypes: the formation of heterocysts, differentiating cells that keep dividing, or the presence of both heterocysts and dividing differentiating cells. The expression of P-het7-gfp in these hetC mutants also showed different patterns of green fluorescent protein (GFP) fluorescence. Thus, the function of hetC is influenced by the genomic background and epistasis and constitutes an example of evolution under way.
SubtypeArticle
KeywordAnabaena Sp Strain Pcc 7120 Microevolution Heterocyst Differentiation
DOI10.1128/JB.00076-18
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationNational Natural Science Foundation of China(31270132 ; National Natural Science Foundation of China(31270132 ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS(2016FBZ09) ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS(2016FBZ09) ; 31770044) ; 31770044) ; National Natural Science Foundation of China(31270132 ; National Natural Science Foundation of China(31270132 ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS(2016FBZ09) ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS(2016FBZ09) ; 31770044) ; 31770044)
Language英语
WOS Research AreaMicrobiology
WOS SubjectMicrobiology
WOS KeywordHETEROCYST PATTERN-FORMATION ; INTERCELLULAR TRANSFER ; INSERTION SEQUENCES ; RESPONSE REGULATOR ; ESCHERICHIA-COLI ; SP PCC-7120 ; DIFFERENTIATION ; GENE ; EXPRESSION ; EVOLUTION
WOS IDWOS:000434807300004
Funding OrganizationNational Natural Science Foundation of China(31270132 ; National Natural Science Foundation of China(31270132 ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS(2016FBZ09) ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS(2016FBZ09) ; 31770044) ; 31770044) ; National Natural Science Foundation of China(31270132 ; National Natural Science Foundation of China(31270132 ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS(2016FBZ09) ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS(2016FBZ09) ; 31770044) ; 31770044)
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/50866
Collection藻类生物学及应用研究中心
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Hubei, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Wang, Yali,Gao, Yuan,Gao, Hong,et al. Three Substrains of the Cyanobacterium Anabaena sp Strain PCC 7120 Display Divergence in Genomic Sequences and hetC Function[J]. JOURNAL OF BACTERIOLOGY,2018,200(13).
APA Wang, Yali,Gao, Yuan,Gao, Hong,Zhang, Cheng-Cai,&Xu, Xudong.(2018).Three Substrains of the Cyanobacterium Anabaena sp Strain PCC 7120 Display Divergence in Genomic Sequences and hetC Function.JOURNAL OF BACTERIOLOGY,200(13).
MLA Wang, Yali,et al."Three Substrains of the Cyanobacterium Anabaena sp Strain PCC 7120 Display Divergence in Genomic Sequences and hetC Function".JOURNAL OF BACTERIOLOGY 200.13(2018).
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