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题名: The discrepancy function of NLRC5 isoforms in antiviral and antibacterial immune responses
作者: Cao, Lu1, 3; Wu, Xiao Man1; Hu, Yi Wei1; Xue, Na Na1; Nie, Pin1, 2; Chang, Ming Xian1, 2
关键词: Zebrafish ; NLRC5 isoforms ; Viral infection ; Bacterial infection ; TLRs signaling ; NF-kappa B signaling
刊名: DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
发表日期: 2018-07-01
DOI: 10.1016/j.dci.2018.02.013
卷: 84, 页:153-163
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Fisheries ; Immunology ; Zoology
研究领域[WOS]: Fisheries ; Immunology ; Zoology
英文摘要: NOD-like receptors (NLRs) are a family of intracellular pattern recognition receptors (PRRs) that play critical roles in innate immunity against pathogens infection. NLRC5, the largest member of NLR family, has been characterized as a regulator of innate immunity and MHC class I expression. Alternative splicing of NLRC5 is only reported in human and zebrafish. However, the function of NLRC5 isoforms in the innate immune responses remains unknown. In the present study, we report the functional characterization of zfNLRC5a and zfNLRC5d, two splicing isoforms of zebrafish NLRC5. zfNLRC5a and zfNLRC5d are generated by exon skipping, and whose alternative splicing sites exist in the region of LRRs. Fluorescence microscopy showed that zfNLRC5 isoforms were located throughout the entire cell including nuclear staining. The expression of zfNLRC5 isoform was inducible in response to bacterial and viral infections. During SVCV infection, the in vitro and in vivo studies found that zfNLRC5d overexpression increased protection against viral infection; however zfNLRC5a overexpression had no significant effect on antiviral activity. Interestingly, zfNLRC5 isoforms but not zfNLRC5 were involved in transcriptional regulation of TLRs and NF-kappa B signaling. Overexpression of zfNLRC5 isoforms also contributed to negative regulation of antibacterial immune response, with the decreased expression of nfkbiaa (Mkt). All together, these results firstly demonstrate the function of NLRC5 isoforms in antiviral and antibacterial immune responses both in vitro and in vivo. (C) 2018 Elsevier Ltd. All rights reserved.
关键词[WOS]: NF-KAPPA-B ; NOD-LIKE RECEPTORS ; PLANT-DISEASE RESISTANCE ; LEUCINE-RICH REPEAT ; TOLL-LIKE RECEPTORS ; INNATE IMMUNITY ; RECOGNITION RECEPTORS ; PATHOGEN RECOGNITION ; RAINBOW-TROUT ; HOST-DEFENSE
语种: 英语
项目资助者: National Natural Science Foundation of China(31372531 ; 31672687)
WOS记录号: WOS:000431160800015
ISSN号: 0145-305X
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内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/50842
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
2.Minist Agr, Key Lab Aquaculture Dis Control, Wuhan 430072, Hubei, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
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