中国科学院水生生物研究所机构知识库
Advanced  
IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
题名: Methionine hydroxy analogue supplementation modulates gill immunological and barrier health status of grass carp (Ctenopharyngodon idella)
作者: Wu, Pei1, 2, 3; Pan, Fei-Yu1; Feng, Lin1, 2, 3; Jiang, Wei-Dan1, 2, 3; Jiang, Jun1, 2, 3; Kuang, Sheng-Yao4; Tang, Ling4; Tang, Wu-Neng4; Zhang, Yong-An5; Zhou, Xiao-Qiu1, 2, 3; Liu, Yang1, 2, 3
关键词: Methionine hydroxy analogue ; Ctenopharyngodon idella ; Gill ; Physical barrier ; Immune defence
刊名: FISH & SHELLFISH IMMUNOLOGY
发表日期: 2018-03-01
DOI: 10.1016/j.fsi.2018.01.031
卷: 74, 页:637-648
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
研究领域[WOS]: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
英文摘要: This study was conducted to investigate the effects of methionine hydroxy analogue (MHA) on the physical barrier and immune defence in the gill of young grass carp (Ctenophcuyngodon idella). A total 630 young grass carp with an average initial weight of 259.70 +/- 0.47 g were fed graded levels of MHA (0, 2.4, 4.4, 6.4, 8.5 and 10.5 g/kg diet) and one DL-methionine (DLM) group (6.4 g/kg diet) for 8 weeks. After feeding trial, 15 fish from each treatment were challenged with Flavobacterium columnare. Compared to the basal diet, optimal MHA improved cellular structure integrity of gill via repressing death receptor and mitochondria pathways induced apoptosis, which might be related to the down-regulation of c-Jun-N-terminal kinase mRNA levels (P < .05). Simultaneously, optimal MHA supplementation improved cellular structure integrity of gill via elevating glutathione contents, antioxidant enzymes activities and corresponding isoforms mRNA levels to attenuate oxidalive damage, which might be to the up-regulation of NF-E2-related factor 2 mRNA levels and down-regulation of Kelch-like ECH-associating protein la mRNA levels (P < .05). Besides, optimal MHA improved intercellular structure integrity of immune organs via up-regulating the mRNA levels of intercellular tight junctions-related genes, which might be owing to the down-regulation of myosin light chain kinase (MLCK) mRNA levels (P < .05). Summarily, MHA could improve the physical barrier of fish gill. In addition, optimal MHA supplementation increased lysozyme (LZ) and acid phosphatase (ACP) activities, complement 3 (C3), C4 and immunoglobulin M contents and up-regulated mRNA levels of liver-expressed antimicrobial peptide 2, hepcidin and beta-defensin, suggesting that MHA could enhance antimicrobial ability of fish gill. Meanwhile, optimal MHA supplementation enhanced the immune defence of gill via down-regulating pro-inflammatory cytokines mRNA levels and up-regulated anti-inflammatory cytokines mRNA levels, which might be attributed to the down regulation of nuclear factor kappa B p65, c-Rel, I kappa B kinase beta, p38 mitogen activated protein kinase, eIF4E-binding proteins (4E-BP1) and 4E-BP2 mRNA levels and up-regulation of inhibitor of kappa B alpha, ribosomal protein S6 kinase 1 and target of rapamycin mRNA levels (P < .05). In conclusion, the positive effect of MHA on gill health is associated with the improvement of the defence against apoptosis, antioxidant status, tight junctions and immune defence of fish gill. Meanwhile, MHA was superior to DLM on improving the physical barrier of fish gill. For the direction to healthy breeding of young grass carp, the optimal MHA supplementation levels on the premise of 4.01 g/kg methionine basal were estimated by quadratic regression curve, such as 5.49, 6.17 and 6.02 g/kg diet bases on the defence against gill-rot, malondialdehyde content and LZ activity in the gill, respectively.
关键词[WOS]: NF-KAPPA-B ; MESSENGER-RNA LEVELS ; BLOOD-BRAIN-BARRIER ; 2-HYDROXY-4-(METHYLTHIO) BUTANOIC ACID ; ANTIOXIDANT ENZYME-ACTIVITIES ; TIGHT JUNCTION PROTEINS ; MUCOSAL IMMUNE-SYSTEM ; APOLIPOPROTEIN-A-I ; GENE-EXPRESSION ; JUVENILE JIAN
语种: 英语
项目资助者: National Basic Research Program of China (973 Program)(2014CB138600) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; Earmarked Fund for China Agriculture Research System(CARS-45) ; Specialized Research Fund for the Doctoral Program of Higher Education of China(20135103110001) ; Outstanding Talents and Innovation Team of Agriculture Scientific Research (Ministry of Agriculture) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Foundation of Sichuan Youth Science and Technology Innovation Research Team(2017TD0002) ; 2013NC0045)
WOS记录号: WOS:000427344200068
ISSN号: 1050-4648
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/50700
Appears in Collections:水生生物分子与细胞生物学研究中心_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Sichuan Agr Univ, Anim Nutr Inst, Chengdu 611130, Sichuan, Peoples R China
2.Sichuan Agr Univ, Fish Nutr & Safety Prod Univ, Key Lab Sichuan Prov, Chengdu 611130, Sichuan, Peoples R China
3.Sichuan Agr Univ, China Minist Educ, Key Lab Anim Dis Resistance Nutr, Chengdu 611130, Sichuan, Peoples R China
4.Sichuan Acad Anim Sci, Anim Nutr Inst, Chengdu 610066, Sichuan, Peoples R China
5.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Wu, Pei]'s Articles
[Pan, Fei-Yu]'s Articles
[Feng, Lin]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Wu, Pei]‘s Articles
[Pan, Fei-Yu]‘s Articles
[Feng, Lin]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2018  中国科学院水生生物研究所 - Feedback
Powered by CSpace