Methionine hydroxy analogue supplementation modulates gill immunological and barrier health status of grass carp (Ctenopharyngodon idella)
Wu, Pei1,2,3; Pan, Fei-Yu1; Feng, Lin1,2,3; Jiang, Wei-Dan1,2,3; Jiang, Jun1,2,3; Kuang, Sheng-Yao4; Tang, Ling4; Tang, Wu-Neng4; Zhang, Yong-An5; Zhou, Xiao-Qiu1,2,3; Liu, Yang1,2,3
2018-03-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume74Pages:637-648
AbstractThis study was conducted to investigate the effects of methionine hydroxy analogue (MHA) on the physical barrier and immune defence in the gill of young grass carp (Ctenophcuyngodon idella). A total 630 young grass carp with an average initial weight of 259.70 +/- 0.47 g were fed graded levels of MHA (0, 2.4, 4.4, 6.4, 8.5 and 10.5 g/kg diet) and one DL-methionine (DLM) group (6.4 g/kg diet) for 8 weeks. After feeding trial, 15 fish from each treatment were challenged with Flavobacterium columnare. Compared to the basal diet, optimal MHA improved cellular structure integrity of gill via repressing death receptor and mitochondria pathways induced apoptosis, which might be related to the down-regulation of c-Jun-N-terminal kinase mRNA levels (P < .05). Simultaneously, optimal MHA supplementation improved cellular structure integrity of gill via elevating glutathione contents, antioxidant enzymes activities and corresponding isoforms mRNA levels to attenuate oxidalive damage, which might be to the up-regulation of NF-E2-related factor 2 mRNA levels and down-regulation of Kelch-like ECH-associating protein la mRNA levels (P < .05). Besides, optimal MHA improved intercellular structure integrity of immune organs via up-regulating the mRNA levels of intercellular tight junctions-related genes, which might be owing to the down-regulation of myosin light chain kinase (MLCK) mRNA levels (P < .05). Summarily, MHA could improve the physical barrier of fish gill. In addition, optimal MHA supplementation increased lysozyme (LZ) and acid phosphatase (ACP) activities, complement 3 (C3), C4 and immunoglobulin M contents and up-regulated mRNA levels of liver-expressed antimicrobial peptide 2, hepcidin and beta-defensin, suggesting that MHA could enhance antimicrobial ability of fish gill. Meanwhile, optimal MHA supplementation enhanced the immune defence of gill via down-regulating pro-inflammatory cytokines mRNA levels and up-regulated anti-inflammatory cytokines mRNA levels, which might be attributed to the down regulation of nuclear factor kappa B p65, c-Rel, I kappa B kinase beta, p38 mitogen activated protein kinase, eIF4E-binding proteins (4E-BP1) and 4E-BP2 mRNA levels and up-regulation of inhibitor of kappa B alpha, ribosomal protein S6 kinase 1 and target of rapamycin mRNA levels (P < .05). In conclusion, the positive effect of MHA on gill health is associated with the improvement of the defence against apoptosis, antioxidant status, tight junctions and immune defence of fish gill. Meanwhile, MHA was superior to DLM on improving the physical barrier of fish gill. For the direction to healthy breeding of young grass carp, the optimal MHA supplementation levels on the premise of 4.01 g/kg methionine basal were estimated by quadratic regression curve, such as 5.49, 6.17 and 6.02 g/kg diet bases on the defence against gill-rot, malondialdehyde content and LZ activity in the gill, respectively.
SubtypeArticle
KeywordMethionine Hydroxy Analogue Ctenopharyngodon Idella Gill Physical Barrier Immune Defence
DOI10.1016/j.fsi.2018.01.031
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationNational Basic Research Program of China (973 Program)(2014CB138600) ; National Basic Research Program of China (973 Program)(2014CB138600) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; Earmarked Fund for China Agriculture Research System(CARS-45) ; Earmarked Fund for China Agriculture Research System(CARS-45) ; Specialized Research Fund for the Doctoral Program of Higher Education of China(20135103110001) ; Specialized Research Fund for the Doctoral Program of Higher Education of China(20135103110001) ; Outstanding Talents and Innovation Team of Agriculture Scientific Research (Ministry of Agriculture) ; Outstanding Talents and Innovation Team of Agriculture Scientific Research (Ministry of Agriculture) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Foundation of Sichuan Youth Science and Technology Innovation Research Team(2017TD0002) ; Foundation of Sichuan Youth Science and Technology Innovation Research Team(2017TD0002) ; 2013NC0045) ; 2013NC0045) ; National Basic Research Program of China (973 Program)(2014CB138600) ; National Basic Research Program of China (973 Program)(2014CB138600) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; Earmarked Fund for China Agriculture Research System(CARS-45) ; Earmarked Fund for China Agriculture Research System(CARS-45) ; Specialized Research Fund for the Doctoral Program of Higher Education of China(20135103110001) ; Specialized Research Fund for the Doctoral Program of Higher Education of China(20135103110001) ; Outstanding Talents and Innovation Team of Agriculture Scientific Research (Ministry of Agriculture) ; Outstanding Talents and Innovation Team of Agriculture Scientific Research (Ministry of Agriculture) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Foundation of Sichuan Youth Science and Technology Innovation Research Team(2017TD0002) ; Foundation of Sichuan Youth Science and Technology Innovation Research Team(2017TD0002) ; 2013NC0045) ; 2013NC0045)
Language英语
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS KeywordNF-KAPPA-B ; MESSENGER-RNA LEVELS ; BLOOD-BRAIN-BARRIER ; 2-HYDROXY-4-(METHYLTHIO) BUTANOIC ACID ; ANTIOXIDANT ENZYME-ACTIVITIES ; TIGHT JUNCTION PROTEINS ; MUCOSAL IMMUNE-SYSTEM ; APOLIPOPROTEIN-A-I ; GENE-EXPRESSION ; JUVENILE JIAN
WOS IDWOS:000427344200068
Funding OrganizationNational Basic Research Program of China (973 Program)(2014CB138600) ; National Basic Research Program of China (973 Program)(2014CB138600) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; Earmarked Fund for China Agriculture Research System(CARS-45) ; Earmarked Fund for China Agriculture Research System(CARS-45) ; Specialized Research Fund for the Doctoral Program of Higher Education of China(20135103110001) ; Specialized Research Fund for the Doctoral Program of Higher Education of China(20135103110001) ; Outstanding Talents and Innovation Team of Agriculture Scientific Research (Ministry of Agriculture) ; Outstanding Talents and Innovation Team of Agriculture Scientific Research (Ministry of Agriculture) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Foundation of Sichuan Youth Science and Technology Innovation Research Team(2017TD0002) ; Foundation of Sichuan Youth Science and Technology Innovation Research Team(2017TD0002) ; 2013NC0045) ; 2013NC0045) ; National Basic Research Program of China (973 Program)(2014CB138600) ; National Basic Research Program of China (973 Program)(2014CB138600) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; Earmarked Fund for China Agriculture Research System(CARS-45) ; Earmarked Fund for China Agriculture Research System(CARS-45) ; Specialized Research Fund for the Doctoral Program of Higher Education of China(20135103110001) ; Specialized Research Fund for the Doctoral Program of Higher Education of China(20135103110001) ; Outstanding Talents and Innovation Team of Agriculture Scientific Research (Ministry of Agriculture) ; Outstanding Talents and Innovation Team of Agriculture Scientific Research (Ministry of Agriculture) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Foundation of Sichuan Youth Science and Technology Innovation Research Team(2017TD0002) ; Foundation of Sichuan Youth Science and Technology Innovation Research Team(2017TD0002) ; 2013NC0045) ; 2013NC0045)
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/50700
Collection藻类生物学及应用研究中心_水生生物分子与细胞生物学研究中心
Affiliation1.Sichuan Agr Univ, Anim Nutr Inst, Chengdu 611130, Sichuan, Peoples R China
2.Sichuan Agr Univ, Fish Nutr & Safety Prod Univ, Key Lab Sichuan Prov, Chengdu 611130, Sichuan, Peoples R China
3.Sichuan Agr Univ, China Minist Educ, Key Lab Anim Dis Resistance Nutr, Chengdu 611130, Sichuan, Peoples R China
4.Sichuan Acad Anim Sci, Anim Nutr Inst, Chengdu 610066, Sichuan, Peoples R China
5.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Wu, Pei,Pan, Fei-Yu,Feng, Lin,et al. Methionine hydroxy analogue supplementation modulates gill immunological and barrier health status of grass carp (Ctenopharyngodon idella)[J]. FISH & SHELLFISH IMMUNOLOGY,2018,74:637-648.
APA Wu, Pei.,Pan, Fei-Yu.,Feng, Lin.,Jiang, Wei-Dan.,Jiang, Jun.,...&Liu, Yang.(2018).Methionine hydroxy analogue supplementation modulates gill immunological and barrier health status of grass carp (Ctenopharyngodon idella).FISH & SHELLFISH IMMUNOLOGY,74,637-648.
MLA Wu, Pei,et al."Methionine hydroxy analogue supplementation modulates gill immunological and barrier health status of grass carp (Ctenopharyngodon idella)".FISH & SHELLFISH IMMUNOLOGY 74(2018):637-648.
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