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题名: Transcriptomics Sequencing Provides Insights into Understanding the Mechanism of Grass Carp Reovirus Infection
作者: Chen, Geng1, 2; He, Libo1; Luo, Lifei1, 2; Huang, Rong1; Liao, Lanjie1; Li, Yongming1; Zhu, Zuoyan1; Wang, Yaping
关键词: grass carp ; grass carp reovirus ; transcriptomics sequencing ; phagosome
刊名: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
发表日期: 2018-02-01
DOI: 10.3390/ijms19020488
卷: 19, 期:2
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]: Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
英文摘要: Grass carp is an important aquaculture fish species in China that is affected by severe diseases, especially haemorrhagic disease caused by grass carp reovirus (GCRV). However, the mechanisms of GCRV invasion and infection remain to be elucidated. In the present study, Ctenopharyngodon idellus kidney (CIK) cells were infected with GCRV, harvested at 0, 8, 24, and 72 h post infection, respectively, and then subjected to transcriptomics sequencing. Each sample yielded more than 6 Gb of clean data and 40 million clean reads. To better understand GCRV infection, the process was divided into three phases: the early (0-8 h post infection), middle (8-24 h post infection), and late (24-72 h) stages of infection. A total of 76 (35 up-regulated, 41 down-regulated), 553 (463 up-regulated, 90 down-regulated), and 284 (150 up-regulated, 134 down-regulated) differently expressed genes (DEGs) were identified during the early, middle, and late stages of infection, respectively. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis revealed that DEGs were mainly involved in carbohydrate biosynthesis, transport, and endocytosis in the early stage, phagocytosis and lysosome pathways were mainly enriched in the middle stage, and programmed cell death, apoptosis, and inflammation were largely associated with the late stage. These results suggest GCRV infection is a gradual process involving adsorption on the cell surface, followed by endocytosis into cells, transport by lysosomes, and eventually resulted in cell necrosis and/or apoptosis. Our findings provide insight into the mechanisms of grass carp reovirus infection.
关键词[WOS]: SURFACE HEPARAN-SULFATE ; FOCAL ADHESION KINASE ; CTENOPHARYNGODON-IDELLUS ; SUBUNIT VACCINE ; VIRUS-INFECTION ; RNA-SEQ ; IMMUNITY ; AUTOIMMUNITY ; RECEPTORS ; ROLES
语种: 英语
项目资助者: National Natural Science Foundation of China(31721005 ; Strategic Priority Research Program of Chinese Academy of Sciences(XDA08030203) ; Hubei Provincial Natural Science Foundation of China(2015CFA007) ; 31702332)
WOS记录号: WOS:000427527400172
ISSN号: 1422-0067
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内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/50694
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing 101408, Peoples R China
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