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题名: Disaccharide combinations and the expression of enolase3 and plasma membrane Ca2+ ATPase isoform in sturgeon sperm cryopreservation
作者: Xi, M. D.1, 2, 3, 4; Li, P.3, 4, 5; Du, H.3, 4; Qiao, X. M.3, 4; Liu, Z. G.3, 4; Wei, Q. W.1, 2, 3, 4
关键词: cryopreservation ; disaccharides ; enolase3 ; plasma membrane Ca2+-ATPase isoform ; sperm
刊名: REPRODUCTION IN DOMESTIC ANIMALS
发表日期: 2018-04-01
DOI: 10.1111/rda.13134
卷: 53, 期:2, 页:472-483
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Agriculture, Dairy & Animal Science ; Reproductive Biology ; Veterinary Sciences
研究领域[WOS]: Agriculture ; Reproductive Biology ; Veterinary Sciences
英文摘要: ContentsAcipenser sinensis and Acipenser dabryanus are critically endangered species, so germplasm conservation via cryopreservation of sperm is necessary. Disaccharides can act as membrane-impermeable cryoprotectants, and enolase3 (ENO3) and plasma membrane Ca2+ ATPase isoform (PMCA2) are proteins associated with sperm quality. We considered seven characteristics of sperm quality in cultured brood stock from A.sinensis and A.dabryanus. We tested use of sucrose or trehalose alone and in combination at different concentrations for cryopreservation of A.dabryanus sperm. A low concentration of sucrose plus trehalose (S15T15) was optimal. Mixing of the extender with sucrose, lactose, or trehalose alone or with pairwise mixtures revealed that a mixture of lactose and trehalose (L15T15) gave the best results for both A.sinensis and A.dabryanus. Enolase3 and PMCA2 expression levels were measured in cryopreserved A.sinensis sperm via Western blotting. Relative ENO3 and PMCA2 expression levels were examined, and the relationship between disaccharide composition, sperm quality and protein expression was explored in A.sinensis. The results showed that relative ENO3 and PMCA2 expression levels were the highest at L15T15 in cryopreserved A.sinensis sperm. There were significant positive correlations between ENO3 expression and percentage membrane integrity, and between PMCA2 expression and sperm motility parameters (percentage of motile sperm, curvilinear velocity, straight-line velocity and average path velocity; p<.05) in cryopreserved A.sinensis sperm. Our results indicate the optimal disaccharide combination and concentrations for cryopreservation of A.sinensis and A.dabryanus sperm and suggest that ENO3 and PMCA2 expression levels could serve as a valuable indicator of sperm quality in A.sinensis.
关键词[WOS]: BULL SPERM ; MOTILITY CHARACTERISTICS ; ACIPENSER-PERSICUS ; GOAT SPERMATOZOA ; POSTTHAW STORAGE ; MALE-FERTILITY ; RAM SEMEN ; EGG-YOLK ; TREHALOSE ; VIABILITY
语种: 英语
项目资助者: National Natural Science Foundation of China(31402301) ; Special Scientific Research Fund for Central Non-profit Institutes, Chinese Academy of Fishery Sciences(2015B02YQ01 ; National Program on Key Basic Research Project(2015CB150702) ; Special Fund for Agro-scientific Research in the Public Interest of the Ministry of Agriculture of China(201203086) ; Ministry of Education, Youth and Sports of the Czech Republic-project "CENAKVA"(CZ.1.05/2.1.00/01.0024) ; Ministry of Education, Youth and Sports of the Czech Republic-project CENAKVA II(LO1205) ; Czech Science Foundation(16-02407Y) ; 2016GH04)
WOS记录号: WOS:000427140800025
ISSN号: 0936-6768
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内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/50692
Appears in Collections:其他_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Key Lab Freshwater Biodivers Conservat, Minist Agr China, Wuhan, Hubei, Peoples R China
4.Chinese Acad Fishery Sci, Sino Czech Joint Lab Fish Conservat & Biotechnol, Yangtze River Fisheries Res Inst, Wuhan, Hubei, Peoples R China
5.Univ South Bohemia Ceske Budejovice, Fac Fisheries & Protect Waters, South Bohemian Res Ctr Aquaculture & Biodivers Hy, Res Inst Fish Culture & Hydrobiol, Vodany, Czech Republic
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