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题名: N-Glycoproteomic Characterization of Mannosidase and Xylosyltransferase Mutant Strains of Chlamydomonas reinhardtii
作者: Schulze, Stefan1, 4; Oltmanns, Anne1; Machnik, Nick1; Liu, Gai2; Xu, Nannan2, 3; Jarmatz, Niklas1; Scholz, Martin1; Sugimoto, Kazuhiko1; Fufezan, Christian1; Huang, Kaiyao2; Hippler, Michael1
刊名: PLANT PHYSIOLOGY
发表日期: 2018-03-01
DOI: 10.1104/pp.17.01450
卷: 176, 期:3, 页:1952-1964
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Plant Sciences
研究领域[WOS]: Plant Sciences
英文摘要: At present, only little is known about the enzymatic machinery required for N-glycosylation in Chlamydomonas reinhardtii, leading to the formation of N-glycans harboring Xyl and methylated Man. This machinery possesses new enzymatic features, as C. reinhardtii N-glycans are independent of beta 1,2-N-acetylglucosaminyltransferase I. Here we have performed comparative N-glycoproteomic analyses of insertional mutants of mannosidase 1A (IMMan1A) and xylosyltransferase 1A (IMXylT1A). The disruption of man1A affected methylation of Man and the addition of terminal Xyl. The absence of XylT1A led to shorter Nglycans compared to the wild type. The use of a IM(Man1A)xIM(XylT1A) double mutant revealed that the absence of Man1A suppressed the IMXylT1A phenotype, indicating that the increased N-glycan trimming is regulated by core beta 1,2-Xyl and is dependent on Man1A activity. These data point toward an enzymatic cascade in the N-glycosylation pathway of C. reinhardtii with interlinked roles of Man1A and XylT1A. The results described herein represent the first step toward a functional characterization of the enzymatic N-glycosylation machinery in C. reinhardtii.
关键词[WOS]: MASS-SPECTROMETRY DATA ; ARABIDOPSIS-THALIANA ; GLYCOSYLATION PATHWAY ; PYTHON MODULE ; GLYCANS ; UNIVERSAL ; DATABASE ; SEARCH ; GENERATION ; MATURATION
语种: 英语
项目资助者: Deutsche Forschungsgemeinschaft(HI 739/12-1) ; Sino-German Center, Beijing, China(GZ990) ; National Natural Science Foundation of China(31371354 ; 31400654)
WOS记录号: WOS:000426848300012
ISSN号: 0032-0889
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内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/50688
Appears in Collections:藻类生物学及应用研究中心_期刊论文

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作者单位: 1.Univ Munster, Inst Plant Biol & Biotechnol, D-48143 Munster, Germany
2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Hubei, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
4.Univ Penn, Dept Biol, Philadelphia, PA 19104 USA
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