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题名: Acetylome Profiling Reveals Extensive Lysine Acetylation of the Fatty Acid Metabolism Pathway in the Diatom Phaeodactylum tricornutum
作者: Chen, Zhuo1, 2; Luo, Ling1; Chen, Runfa1; Hu, Hanhua3; Pan, Yufang3; Jiang, Haibo4, 5; Wan, Xia1; Jin, Hu3; Gong, Yangmin1
刊名: MOLECULAR & CELLULAR PROTEOMICS
发表日期: 2018-03-01
DOI: 10.1074/mcp.RA117.000339
卷: 17, 期:3, 页:399-412
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Biochemical Research Methods
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: N-epsilon-lysine acetylation represents a highly dynamic and reversibly regulated post-translational modification widespread in almost all organisms, and plays important roles for regulation of protein function in diverse metabolic pathways. However, little is known about the role of lysine acetylation in photosynthetic eukaryotic microalgae. We integrated proteomic approaches to comprehensively characterize the lysine acetylome in the model diatom Phaeodactylum tricornutum. In total, 2324 acetylation sites from 1220 acetylated proteins were identified, representing the largest data set of the lysine acetylome in plants to date. Almost all enzymes involved in fatty acid synthesis were found to be lysine acetylated. Six putative lysine acetylation sites were identified in a plastid-localized long-chain acyl-CoA synthetase. Site-directed mutagenesis and site-specific incorporation of N-acetyllysine in acyl-CoA synthetase show that acetylation at K407 and K425 increases its enzyme activity. Moreover, the nonenzymatically catalyzed overall hyperacetylation of acyl-CoA synthetase by acetyl-phosphate can be effectively deacetylated and reversed by a sirtuin-type NAD(+)-dependent deacetylase with subcellular localization of both the plastid and nucleus in Phaeodactylum. This work indicates the regulation of acyl-CoA synthetase activity by site-specific lysine acetylation and highlights the potential regulation of fatty acid metabolism by lysine actetylation in the plastid of the diatom Phaeodactylum.
关键词[WOS]: CHAIN ACYL-COENZYME ; RHODOPSEUDOMONAS-PALUSTRIS ; PROTEIN-PHOSPHORYLATION ; FUNCTIONAL GENOMICS ; SALMONELLA-ENTERICA ; COA SYNTHETASE ; PCC 7002 ; IDENTIFICATION ; ARABIDOPSIS ; PHOSPHOPROTEOME
语种: 英语
项目资助者: Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences(CAAS-ASTIP-2013-OCRI) ; National Natural Science Foundation of China(31370746)
WOS记录号: WOS:000426370400003
ISSN号: 1535-9476
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内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/50676
Appears in Collections:藻类生物学及应用研究中心_期刊论文

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作者单位: 1.Chinese Acad Agr Sci, Minist Agr, Oil Crops Res Inst, Key Lab Biol & Genet Improvement Oil Crops, Wuhan 430062, Hubei, Peoples R China
2.Shandong Normal Univ, Coll Life Sci, Key Lab Plant Stress Res, Jinan 250014, Shandong, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Hubei, Peoples R China
4.Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China
5.Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China
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