Lysine Succinylation Contributes to Aflatoxin Production and Pathogenicity in Aspergillus flavus
Ren, Silin1,2; Yang, Mingkun3; Yue, Yuewei1,2; Ge, Feng3; Li, Yu1,2; Guo, Xiaodong1,2; Zhang, Jia3; Zhang, Feng1,2; Nie, Xinyi1,2; Wang, Shihua1,2
2018-03-01
Source PublicationMOLECULAR & CELLULAR PROTEOMICS
ISSN1535-9476
Volume17Issue:3Pages:457-471
AbstractAspergillus flavus (A. flavus) is a ubiquitous saprophytic and pathogenic fungus that produces the aflatoxin carcinogen, and A. flavus can have tremendous economic and health impacts worldwide. Increasing evidence demonstrates that lysine succinylation plays an important regulatory role in metabolic processes in both bacterial and human cells. However, little is known about the extent and function of lysine succinylation in A. flavus. Here, we performed a global succinylome analysis of A. flavus using high accuracy nano-LC-MS/MS in combination with the enrichment of succinylated peptides from digested cell lysates and subsequent peptide identification. In total, 985 succinylation sites on 349 succinylated proteins were identified in this pathogen. Bioinformatics analysis revealed that the succinylated proteins were involved in various biological processes and were particularly enriched in the aflatoxin biosynthesis process. Site-specific mutagenesis and biochemical studies showed that lysine succinylation on the norsolorinic acid reductase NorA (AflE), a key enzyme in aflatoxins biosynthesis, can affect the production of sclerotia and aflatoxins biosynthesis in A. flavus. Together, our findings reveal widespread roles for lysine succinylation in regulating metabolism and aflatoxins biosynthesis in A. flavus. Our data provide a rich resource for functional analyses of lysine succinylation and facilitate the dissection of metabolic networks in this pathogen.
SubtypeArticle
DOI10.1074/mcp.RA117.000393
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationNational Basic Research Program of China(2013CB127802) ; National Basic Research Program of China(2013CB127802) ; National Natural Science Foundation of China(31172297 ; National Natural Science Foundation of China(31172297 ; 31400100) ; 31400100) ; National Basic Research Program of China(2013CB127802) ; National Basic Research Program of China(2013CB127802) ; National Natural Science Foundation of China(31172297 ; National Natural Science Foundation of China(31172297 ; 31400100) ; 31400100)
Language英语
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemical Research Methods
WOS KeywordPROTEIN POSTTRANSLATIONAL MODIFICATIONS ; ACETYLOME ANALYSIS REVEALS ; SECONDARY METABOLISM ; MYCOBACTERIUM-TUBERCULOSIS ; SCLEROTIAL PRODUCTION ; INTERACTION NETWORKS ; SUBCELLULAR-LOCALIZATION ; TOXOPLASMA-GONDII ; DIVERSE FUNCTIONS ; OXIDATIVE STRESS
WOS IDWOS:000426370400008
Funding OrganizationNational Basic Research Program of China(2013CB127802) ; National Basic Research Program of China(2013CB127802) ; National Natural Science Foundation of China(31172297 ; National Natural Science Foundation of China(31172297 ; 31400100) ; 31400100) ; National Basic Research Program of China(2013CB127802) ; National Basic Research Program of China(2013CB127802) ; National Natural Science Foundation of China(31172297 ; National Natural Science Foundation of China(31172297 ; 31400100) ; 31400100)
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Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/50659
Collection藻类生物学及应用研究中心_水生生物分子与细胞生物学研究中心
Affiliation1.Fujian Agr & Forestry Univ, Key Lab Pathogen Fungi & Mycotoxins Fujian Prov, Fuzhou 350002, Fujian, Peoples R China
2.Fujian Agr & Forestry Univ, Sch Life Sci, Fuzhou 350002, Fujian, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Ren, Silin,Yang, Mingkun,Yue, Yuewei,et al. Lysine Succinylation Contributes to Aflatoxin Production and Pathogenicity in Aspergillus flavus[J]. MOLECULAR & CELLULAR PROTEOMICS,2018,17(3):457-471.
APA Ren, Silin.,Yang, Mingkun.,Yue, Yuewei.,Ge, Feng.,Li, Yu.,...&Wang, Shihua.(2018).Lysine Succinylation Contributes to Aflatoxin Production and Pathogenicity in Aspergillus flavus.MOLECULAR & CELLULAR PROTEOMICS,17(3),457-471.
MLA Ren, Silin,et al."Lysine Succinylation Contributes to Aflatoxin Production and Pathogenicity in Aspergillus flavus".MOLECULAR & CELLULAR PROTEOMICS 17.3(2018):457-471.
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