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题名: Functional characterization of a short peptidoglycan recognition protein from Chinese giant salamander (Andrias davidianus)
作者: Qi, Zhitao1; Ren, Shisi2; Zhang, Qihuan1; Zou, Jun3; Xu, Qiaoqing4; Wang, Zisheng1; Qiao, Guo1; Nie, Pin2, 5; Chang, Mingxian2, 5
关键词: peptidoglycan recognition protein ; andrias davidianus ; gene clone ; functional analysis ; Immunology and Microbiology Section ; Immune response ; Immunity
刊名: ONCOTARGET
发表日期: 2017-11-21
DOI: 10.18632/oncotarget.21470
卷: 8, 期:59, 页:99323-99335
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Oncology ; Cell Biology
研究领域[WOS]: Oncology ; Cell Biology
英文摘要: Peptidoglycan (PGN) recognition proteins (PGRPs) are important pattern recognition receptors (PRRs) involved in immune defense against bacterial infections. In this study, a short PGRP (termed AdPGRP-S1) was cloned and functionally characterized from Chinese giant salamander (Andrias davidianus), the largest extant urodela amphibian species. AdPGRP-S1 was 184 aa in length and shared 38.7%-54.9% sequence identities with other vertebrates' short PGRPs. It contained one typical PGRP domain at the C-terminal region and several conserved amino acid (aa) residues involved in amidase and PGN binding. AdPGRP-S1 was constitutively expressed in all tissues examined, with the highest expression level seen in spleen and intestine. It has been shown that AdPGRP-S1 could bind and degrade Lys-PGN and Dap-PGN. Further, AdPGRP-S1 had antibacterial activity against the Gram-negative bacteria, Edwardsiella tarda, and was able to trigger the activation of NF-kappa B signaling. These results demonstrated that AdPGRP-S1 possesses multiple functions in pathogen recognition, mediating ceullular signaling, and initiating antibacterial response. This is the first functional study of a salamander PGRP, providing insight to further understand the functional evolution of verterbates' PGRPs.
关键词[WOS]: CARP CTENOPHARYNGODON-IDELLA ; INNATE IMMUNE RECOGNITION ; TROUT ONCORHYNCHUS-MYKISS ; GRAM-POSITIVE BACTERIA ; L-ALANINE AMIDASE ; EXPRESSION ANALYSIS ; DROSOPHILA-MELANOGASTER ; INFECTION ; PGRPS ; IDENTIFICATION
语种: 英语
项目资助者: National Natural Science Foundation of China(31302221 ; Jiangsu Province(BK20171274 ; Opening Project of Jiangsu Key Laboratory of Biochemistry and Biotechnology of Marine Wetland(K2016-08) ; "Qinglan" project of Jiangsu province of China ; 31172408 ; BK2011418) ; 31272666)
WOS记录号: WOS:000419561600019
ISSN号: 1949-2553
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内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/50649
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Yancheng Inst Technol, Jiangsu Key Lab Biochem & Biotechnol Marine Wetla, Yancheng, Jiangsu, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Hubei, Peoples R China
3.Univ Aberdeen, Scottish Fish Immunol Res Ctr, Aberdeen, Scotland
4.Yangtze Univ, Coll Anim Sci, Jingzhou, Hubei, Peoples R China
5.Minist Agr, Key Lab Aquaculture Dis Control, Wuhan, Hubei, Peoples R China
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