IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心
Functional characterization of a short peptidoglycan recognition protein from Chinese giant salamander (Andrias davidianus)
Qi, Zhitao1; Ren, Shisi2; Zhang, Qihuan1; Zou, Jun3; Xu, Qiaoqing4; Wang, Zisheng1; Qiao, Guo1; Nie, Pin2,5; Chang, Mingxian2,5
2017-11-21
Source PublicationONCOTARGET
ISSN1949-2553
Volume8Issue:59Pages:99323-99335
AbstractPeptidoglycan (PGN) recognition proteins (PGRPs) are important pattern recognition receptors (PRRs) involved in immune defense against bacterial infections. In this study, a short PGRP (termed AdPGRP-S1) was cloned and functionally characterized from Chinese giant salamander (Andrias davidianus), the largest extant urodela amphibian species. AdPGRP-S1 was 184 aa in length and shared 38.7%-54.9% sequence identities with other vertebrates' short PGRPs. It contained one typical PGRP domain at the C-terminal region and several conserved amino acid (aa) residues involved in amidase and PGN binding. AdPGRP-S1 was constitutively expressed in all tissues examined, with the highest expression level seen in spleen and intestine. It has been shown that AdPGRP-S1 could bind and degrade Lys-PGN and Dap-PGN. Further, AdPGRP-S1 had antibacterial activity against the Gram-negative bacteria, Edwardsiella tarda, and was able to trigger the activation of NF-kappa B signaling. These results demonstrated that AdPGRP-S1 possesses multiple functions in pathogen recognition, mediating ceullular signaling, and initiating antibacterial response. This is the first functional study of a salamander PGRP, providing insight to further understand the functional evolution of verterbates' PGRPs.
SubtypeArticle
KeywordPeptidoglycan Recognition Protein Andrias Davidianus Gene Clone Functional Analysis Immunology And Microbiology Section Immune Response Immunity
DOI10.18632/oncotarget.21470
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationNational Natural Science Foundation of China(31302221 ; National Natural Science Foundation of China(31302221 ; Jiangsu Province(BK20171274 ; Jiangsu Province(BK20171274 ; Opening Project of Jiangsu Key Laboratory of Biochemistry and Biotechnology of Marine Wetland(K2016-08) ; Opening Project of Jiangsu Key Laboratory of Biochemistry and Biotechnology of Marine Wetland(K2016-08) ; "Qinglan" project of Jiangsu province of China ; "Qinglan" project of Jiangsu province of China ; 31172408 ; 31172408 ; BK2011418) ; BK2011418) ; 31272666) ; 31272666) ; National Natural Science Foundation of China(31302221 ; National Natural Science Foundation of China(31302221 ; Jiangsu Province(BK20171274 ; Jiangsu Province(BK20171274 ; Opening Project of Jiangsu Key Laboratory of Biochemistry and Biotechnology of Marine Wetland(K2016-08) ; Opening Project of Jiangsu Key Laboratory of Biochemistry and Biotechnology of Marine Wetland(K2016-08) ; "Qinglan" project of Jiangsu province of China ; "Qinglan" project of Jiangsu province of China ; 31172408 ; 31172408 ; BK2011418) ; BK2011418) ; 31272666) ; 31272666)
Language英语
WOS Research AreaOncology ; Cell Biology
WOS SubjectOncology ; Cell Biology
WOS KeywordCARP CTENOPHARYNGODON-IDELLA ; INNATE IMMUNE RECOGNITION ; TROUT ONCORHYNCHUS-MYKISS ; GRAM-POSITIVE BACTERIA ; L-ALANINE AMIDASE ; EXPRESSION ANALYSIS ; DROSOPHILA-MELANOGASTER ; INFECTION ; PGRPS ; IDENTIFICATION
WOS IDWOS:000419561600019
Funding OrganizationNational Natural Science Foundation of China(31302221 ; National Natural Science Foundation of China(31302221 ; Jiangsu Province(BK20171274 ; Jiangsu Province(BK20171274 ; Opening Project of Jiangsu Key Laboratory of Biochemistry and Biotechnology of Marine Wetland(K2016-08) ; Opening Project of Jiangsu Key Laboratory of Biochemistry and Biotechnology of Marine Wetland(K2016-08) ; "Qinglan" project of Jiangsu province of China ; "Qinglan" project of Jiangsu province of China ; 31172408 ; 31172408 ; BK2011418) ; BK2011418) ; 31272666) ; 31272666) ; National Natural Science Foundation of China(31302221 ; National Natural Science Foundation of China(31302221 ; Jiangsu Province(BK20171274 ; Jiangsu Province(BK20171274 ; Opening Project of Jiangsu Key Laboratory of Biochemistry and Biotechnology of Marine Wetland(K2016-08) ; Opening Project of Jiangsu Key Laboratory of Biochemistry and Biotechnology of Marine Wetland(K2016-08) ; "Qinglan" project of Jiangsu province of China ; "Qinglan" project of Jiangsu province of China ; 31172408 ; 31172408 ; BK2011418) ; BK2011418) ; 31272666) ; 31272666)
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/50649
Collection鱼类生物学及渔业生物技术研究中心
Affiliation1.Yancheng Inst Technol, Jiangsu Key Lab Biochem & Biotechnol Marine Wetla, Yancheng, Jiangsu, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Hubei, Peoples R China
3.Univ Aberdeen, Scottish Fish Immunol Res Ctr, Aberdeen, Scotland
4.Yangtze Univ, Coll Anim Sci, Jingzhou, Hubei, Peoples R China
5.Minist Agr, Key Lab Aquaculture Dis Control, Wuhan, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Qi, Zhitao,Ren, Shisi,Zhang, Qihuan,et al. Functional characterization of a short peptidoglycan recognition protein from Chinese giant salamander (Andrias davidianus)[J]. ONCOTARGET,2017,8(59):99323-99335.
APA Qi, Zhitao.,Ren, Shisi.,Zhang, Qihuan.,Zou, Jun.,Xu, Qiaoqing.,...&Chang, Mingxian.(2017).Functional characterization of a short peptidoglycan recognition protein from Chinese giant salamander (Andrias davidianus).ONCOTARGET,8(59),99323-99335.
MLA Qi, Zhitao,et al."Functional characterization of a short peptidoglycan recognition protein from Chinese giant salamander (Andrias davidianus)".ONCOTARGET 8.59(2017):99323-99335.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Qi, Zhitao]'s Articles
[Ren, Shisi]'s Articles
[Zhang, Qihuan]'s Articles
Baidu academic
Similar articles in Baidu academic
[Qi, Zhitao]'s Articles
[Ren, Shisi]'s Articles
[Zhang, Qihuan]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Qi, Zhitao]'s Articles
[Ren, Shisi]'s Articles
[Zhang, Qihuan]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.