Dietary threonine deficiency depressed the disease resistance, immune and physical barriers in the gills of juvenile grass carp (Ctenopharyngodon idella) under infection of Flavobacterium columnare
Dong, Yu-Wen1; Feng, Lin1,2,3; Jiang, Wei-Dan1,2,3; Liu, Yang1,2,3; Wu, Pei1,2,3; Jiang, Jun1,2,3; Kuang, Sheng-Yao4; Tang, Ling4; Tang, Wu-Neng4; Zhang, Yong-An5; Zhou, Xiao-Qiu1,2,3
2018
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume72Pages:161-173
AbstractThis study was conducted to investigate the effects of dietary threonine on the disease resistance, gill immune and physical barriers function of juvenile grass carp (Ctenopharyngodon idella). A total of 1080 juveniles were fed six iso-nitrogenous diets containing graded levels of threonine (3.99-21.66 g kg(-1) diet) for 8 weeks, and then challenged with Flavobacterium colunmore. Results showed that threonine deficiency (3.99 g kg(-1) diet): (1) increased the gill rot morbidity after exposure to F. colwnnare; (2) attenuated the gill immune barrier function by decreasing antimicrobial substances production, up-regulating the mRNA levels of pro-inflammatory cytokines (except IL-12p40), and down-regulating the anti-inflammatory cytokines partly due to the modulation of NF-kappa B and TOR signaling. (3) disrupt the gill tight junction complexes by down-regulating TJs (claudin-3, -b, -c, 12, occludin, ZO-1 and ZO-2) and up-regulating TJs (claudin-7a, -7b) as well as related signaling molecule myosin light chain kinase mRNA levels (P < 0.05). (4) exacerbated the gill apoptosis by up-regulating cysteinyl aspartic acid-protease-3, 8, 9, c-Jun N-terminal kinases and mediating apoptosis related factors mRNA levels (P < 0.05); (5) exacerbated oxidative injury with increased reactive oxygen species, malondialdehyde and protein carbonyl contents (P < 0.05), decreased the antioxidant related enzymes activities and corresponding mRNA levels (except glutathione peroxidase-1b and glutathione-S-transferase-omega 2) as well as glutathione contents (P < 0.05) partly ascribe to the abridgement of NF-E2-related factor 2 signaling [Nrf2/Keapla (not Keap1b)] in fish gill. Overall, threonine deficiency depressed the disease resistance, and impaired immune and physical barriers in fish gill. Finally, based on the gill rot morbidity and biochemical indices (immune indices LA activity and antioxidant indices MDA content), threonine requirements for juvenile grass carp (9.53-53.43 g) were estimated to be 15.32 g kg(-1) diet (4.73 g 100 g(-1) protein), 15.52 g kg(-1) diet (4.79 g 100 g(-1) protein), 15.46 g kg(-1) diet (4.77 g 100 g(-1) protein), respectively.
SubtypeArticle
KeywordThreonine Disease Resistance Immune Barrier Physical Barrier Gill Juvenile Grass Carp (Ctenopharyngodon Idella)
DOI10.1016/j.fsi.2017.10.048
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationNational Natural Science Foundation of China(31572632 ; National Natural Science Foundation of China(31572632 ; National Basic Research Program of China (973 Program)(2014CB138600) ; National Basic Research Program of China (973 Program)(2014CB138600) ; Outstanding Talents and Innovative Team of Agricultural Scientific Research (Ministry of Agriculture) ; Outstanding Talents and Innovative Team of Agricultural Scientific Research (Ministry of Agriculture) ; Science and Technology Support Programme of Sichuan Province of China(2014NZ0003) ; Science and Technology Support Programme of Sichuan Province of China(2014NZ0003) ; Earmarked Fund for China Agriculture Research System(CARS-45) ; Earmarked Fund for China Agriculture Research System(CARS-45) ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2013NC0045) ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2013NC0045) ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Foundation of Sichuan Youth Science and Technology Innovation Research Team(2017TD0002) ; Foundation of Sichuan Youth Science and Technology Innovation Research Team(2017TD0002) ; 31672662) ; 31672662) ; National Natural Science Foundation of China(31572632 ; National Natural Science Foundation of China(31572632 ; National Basic Research Program of China (973 Program)(2014CB138600) ; National Basic Research Program of China (973 Program)(2014CB138600) ; Outstanding Talents and Innovative Team of Agricultural Scientific Research (Ministry of Agriculture) ; Outstanding Talents and Innovative Team of Agricultural Scientific Research (Ministry of Agriculture) ; Science and Technology Support Programme of Sichuan Province of China(2014NZ0003) ; Science and Technology Support Programme of Sichuan Province of China(2014NZ0003) ; Earmarked Fund for China Agriculture Research System(CARS-45) ; Earmarked Fund for China Agriculture Research System(CARS-45) ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2013NC0045) ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2013NC0045) ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Foundation of Sichuan Youth Science and Technology Innovation Research Team(2017TD0002) ; Foundation of Sichuan Youth Science and Technology Innovation Research Team(2017TD0002) ; 31672662) ; 31672662)
Language英语
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS KeywordNF-KAPPA-B ; TIGHT JUNCTION PROTEINS ; ZEBRAFISH DANIO-RERIO ; BREAM MEGALOBRAMA-AMBLYCEPHALA ; CATFISH ICTALURUS-PUNCTATUS ; GENE-EXPRESSION ; ANTIOXIDANT STATUS ; OXIDATIVE STRESS ; CYPRINUS-CARPIO ; GROWTH-PERFORMANCE
WOS IDWOS:000423644600020
Funding OrganizationNational Natural Science Foundation of China(31572632 ; National Natural Science Foundation of China(31572632 ; National Basic Research Program of China (973 Program)(2014CB138600) ; National Basic Research Program of China (973 Program)(2014CB138600) ; Outstanding Talents and Innovative Team of Agricultural Scientific Research (Ministry of Agriculture) ; Outstanding Talents and Innovative Team of Agricultural Scientific Research (Ministry of Agriculture) ; Science and Technology Support Programme of Sichuan Province of China(2014NZ0003) ; Science and Technology Support Programme of Sichuan Province of China(2014NZ0003) ; Earmarked Fund for China Agriculture Research System(CARS-45) ; Earmarked Fund for China Agriculture Research System(CARS-45) ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2013NC0045) ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2013NC0045) ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Foundation of Sichuan Youth Science and Technology Innovation Research Team(2017TD0002) ; Foundation of Sichuan Youth Science and Technology Innovation Research Team(2017TD0002) ; 31672662) ; 31672662) ; National Natural Science Foundation of China(31572632 ; National Natural Science Foundation of China(31572632 ; National Basic Research Program of China (973 Program)(2014CB138600) ; National Basic Research Program of China (973 Program)(2014CB138600) ; Outstanding Talents and Innovative Team of Agricultural Scientific Research (Ministry of Agriculture) ; Outstanding Talents and Innovative Team of Agricultural Scientific Research (Ministry of Agriculture) ; Science and Technology Support Programme of Sichuan Province of China(2014NZ0003) ; Science and Technology Support Programme of Sichuan Province of China(2014NZ0003) ; Earmarked Fund for China Agriculture Research System(CARS-45) ; Earmarked Fund for China Agriculture Research System(CARS-45) ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2013NC0045) ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2013NC0045) ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Foundation of Sichuan Youth Science and Technology Innovation Research Team(2017TD0002) ; Foundation of Sichuan Youth Science and Technology Innovation Research Team(2017TD0002) ; 31672662) ; 31672662)
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/50644
Collection藻类生物学及应用研究中心_水生生物分子与细胞生物学研究中心
Affiliation1.Sichuan Agr Univ, Anim Nutr Inst, Chengdu 611130, Sichuan, Peoples R China
2.Sichuan Agr Univ, Fish Nutr & Safety Prod Univ Key Lab Sichuan Prov, Chengdu 611130, Sichuan, Peoples R China
3.Sichuan Agr Univ, Key Lab Anim Dis Resistance Nutr China, Minist Educ, Chengdu 611130, Sichuan, Peoples R China
4.Sichuan Acad Anim Sci, Anim Nutr Inst, Chengdu 610066, Sichuan, Peoples R China
5.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Dong, Yu-Wen,Feng, Lin,Jiang, Wei-Dan,et al. Dietary threonine deficiency depressed the disease resistance, immune and physical barriers in the gills of juvenile grass carp (Ctenopharyngodon idella) under infection of Flavobacterium columnare[J]. FISH & SHELLFISH IMMUNOLOGY,2018,72:161-173.
APA Dong, Yu-Wen.,Feng, Lin.,Jiang, Wei-Dan.,Liu, Yang.,Wu, Pei.,...&Zhou, Xiao-Qiu.(2018).Dietary threonine deficiency depressed the disease resistance, immune and physical barriers in the gills of juvenile grass carp (Ctenopharyngodon idella) under infection of Flavobacterium columnare.FISH & SHELLFISH IMMUNOLOGY,72,161-173.
MLA Dong, Yu-Wen,et al."Dietary threonine deficiency depressed the disease resistance, immune and physical barriers in the gills of juvenile grass carp (Ctenopharyngodon idella) under infection of Flavobacterium columnare".FISH & SHELLFISH IMMUNOLOGY 72(2018):161-173.
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