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题名: Effects of different cytokines on immune responses of rainbow trout in a virus DNA vaccination model
作者: Cao, Yongsheng1, 2; Zhang, Qiya2; Xu, Liming1; Li, Shaowu1; Wang, Di1; Zhao, Jingzhuang1; Liu, Hongbai1; Feng, Jian3; Lu, Tongyan1
关键词: rainbow trout ; cytokines ; adjuvant ; DNA vaccine ; protection ; Immune response ; Immunology
刊名: ONCOTARGET
发表日期: 2017-12-22
DOI: 10.18632/oncotarget.23095
卷: 8, 期:68, 页:112222-112235
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Oncology ; Cell Biology
研究领域[WOS]: Oncology ; Cell Biology
英文摘要: Seven rainbow trout cytokine genes (interleukin (IL)-2, IL-8, IL-15, IL-17, IL-1 beta, intracellular interferon (iIFN) 1a, and IFN-gamma 2) were evaluated for their adjuvant effects on a DNA vaccine, called pG, containing the glycoprotein gene of infectious hematopoietic necrosis virus (IHNV). Distinct DNA constructs in expression plasmid pcDNA3.1 encoding a cytokine gene were generated. Immunofluorescence assays in rainbow trout gonadal cells demonstrated successful protein expression from all these constructs. Subsequently, fish were immunized with pG alone or together with a cytokine expression plasmid. Results showed that each cytokine plasmids at an appropriate dose showed notable effects on immune gene expression. IL-17 and IFN-gamma 2 can enhance early specific IgM response. All cytokines, except IL-8, can benefit initial neutralizing antibody (NAb) titers. At 35 days post immunization (dpi), NAb titers of fish immunized with pG and IL-2, iIFN1a, or IFN-gamma 2 plasmids remained at high levels (1: 160). NAb titers of fish immunized with pG alone decreased to 1: 40. IL-8 or IL-1 beta can enhance antigen-specific proliferative T-cell responses at 14 dpi. At 28 dpi, coinjection of pG with IL-2, IL-8, IL-15, or IL-17 plasmids induced considerably stronger lymphocyte proliferation than that with injection of pG alone. All cytokine plasmids delivered with pG plasmid enhanced protection of trout against IHNV-mediated mortality. These results indicate that the type and dose of trout cytokine genes injected into fish affect quality of immune response to DNA vaccination.
关键词[WOS]: HEMATOPOIETIC NECROSIS VIRUS ; T-CELL RESPONSES ; ONCORHYNCHUS-MYKISS ; ADJUVANT ACTIVITY ; IN-VIVO ; FISH ; EXPRESSION ; VACCINES ; GENE ; INTERFERON
语种: 英语
项目资助者: Central-level Nonprofit Scientific Research Institutes Special Funds(HSY201702M) ; China Postdoctoral Science Foundation(2016M602395) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2017FB03)
WOS记录号: WOS:000419569800007
ISSN号: 1949-2553
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内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/50632
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Chinese Acad Fishery Sci, Heilongjiang River Fishery Res Inst, Harbin, Heilongjiang, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Hubei, Peoples R China
3.Benxi Agrimarine Co Ltd, Benxi, Peoples R China
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