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Effects of different cytokines on immune responses of rainbow trout in a virus DNA vaccination model
Cao, Yongsheng1,2; Zhang, Qiya2; Xu, Liming1; Li, Shaowu1; Wang, Di1; Zhao, Jingzhuang1; Liu, Hongbai1; Feng, Jian3; Lu, Tongyan1
2017-12-22
Source PublicationONCOTARGET
ISSN1949-2553
Volume8Issue:68Pages:112222-112235
AbstractSeven rainbow trout cytokine genes (interleukin (IL)-2, IL-8, IL-15, IL-17, IL-1 beta, intracellular interferon (iIFN) 1a, and IFN-gamma 2) were evaluated for their adjuvant effects on a DNA vaccine, called pG, containing the glycoprotein gene of infectious hematopoietic necrosis virus (IHNV). Distinct DNA constructs in expression plasmid pcDNA3.1 encoding a cytokine gene were generated. Immunofluorescence assays in rainbow trout gonadal cells demonstrated successful protein expression from all these constructs. Subsequently, fish were immunized with pG alone or together with a cytokine expression plasmid. Results showed that each cytokine plasmids at an appropriate dose showed notable effects on immune gene expression. IL-17 and IFN-gamma 2 can enhance early specific IgM response. All cytokines, except IL-8, can benefit initial neutralizing antibody (NAb) titers. At 35 days post immunization (dpi), NAb titers of fish immunized with pG and IL-2, iIFN1a, or IFN-gamma 2 plasmids remained at high levels (1: 160). NAb titers of fish immunized with pG alone decreased to 1: 40. IL-8 or IL-1 beta can enhance antigen-specific proliferative T-cell responses at 14 dpi. At 28 dpi, coinjection of pG with IL-2, IL-8, IL-15, or IL-17 plasmids induced considerably stronger lymphocyte proliferation than that with injection of pG alone. All cytokine plasmids delivered with pG plasmid enhanced protection of trout against IHNV-mediated mortality. These results indicate that the type and dose of trout cytokine genes injected into fish affect quality of immune response to DNA vaccination.
SubtypeArticle
KeywordRainbow Trout Cytokines Adjuvant Dna Vaccine Protection Immune Response Immunology
DOI10.18632/oncotarget.23095
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationCentral-level Nonprofit Scientific Research Institutes Special Funds(HSY201702M) ; Central-level Nonprofit Scientific Research Institutes Special Funds(HSY201702M) ; China Postdoctoral Science Foundation(2016M602395) ; China Postdoctoral Science Foundation(2016M602395) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2017FB03) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2017FB03) ; Central-level Nonprofit Scientific Research Institutes Special Funds(HSY201702M) ; Central-level Nonprofit Scientific Research Institutes Special Funds(HSY201702M) ; China Postdoctoral Science Foundation(2016M602395) ; China Postdoctoral Science Foundation(2016M602395) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2017FB03) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2017FB03)
Language英语
WOS Research AreaOncology ; Cell Biology
WOS SubjectOncology ; Cell Biology
WOS KeywordHEMATOPOIETIC NECROSIS VIRUS ; T-CELL RESPONSES ; ONCORHYNCHUS-MYKISS ; ADJUVANT ACTIVITY ; IN-VIVO ; FISH ; EXPRESSION ; VACCINES ; GENE ; INTERFERON
WOS IDWOS:000419569800007
Funding OrganizationCentral-level Nonprofit Scientific Research Institutes Special Funds(HSY201702M) ; Central-level Nonprofit Scientific Research Institutes Special Funds(HSY201702M) ; China Postdoctoral Science Foundation(2016M602395) ; China Postdoctoral Science Foundation(2016M602395) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2017FB03) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2017FB03) ; Central-level Nonprofit Scientific Research Institutes Special Funds(HSY201702M) ; Central-level Nonprofit Scientific Research Institutes Special Funds(HSY201702M) ; China Postdoctoral Science Foundation(2016M602395) ; China Postdoctoral Science Foundation(2016M602395) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2017FB03) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2017FB03)
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/50632
Collection鱼类生物学及渔业生物技术研究中心
Affiliation1.Chinese Acad Fishery Sci, Heilongjiang River Fishery Res Inst, Harbin, Heilongjiang, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Hubei, Peoples R China
3.Benxi Agrimarine Co Ltd, Benxi, Peoples R China
Recommended Citation
GB/T 7714
Cao, Yongsheng,Zhang, Qiya,Xu, Liming,et al. Effects of different cytokines on immune responses of rainbow trout in a virus DNA vaccination model[J]. ONCOTARGET,2017,8(68):112222-112235.
APA Cao, Yongsheng.,Zhang, Qiya.,Xu, Liming.,Li, Shaowu.,Wang, Di.,...&Lu, Tongyan.(2017).Effects of different cytokines on immune responses of rainbow trout in a virus DNA vaccination model.ONCOTARGET,8(68),112222-112235.
MLA Cao, Yongsheng,et al."Effects of different cytokines on immune responses of rainbow trout in a virus DNA vaccination model".ONCOTARGET 8.68(2017):112222-112235.
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