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题名: Coordinating carbon and nitrogen metabolic signaling through the cyanobacterial global repressor NdhR
作者: Jiang, Yong-Liang1, 2, 3; Wang, Xue-Ping1, 2, 3; Sun, Hui1, 2, 3; Han, Shu-Jing1, 2, 3; Li, Wei-Fang1, 2, 3; Cui, Ning1, 2, 3; Lin, Gui-Ming4; Zhang, Ju-Yuan4; Cheng, Wang1, 2, 3; Cao, Dong-Dong1, 2, 3; Zhang, Zhi-Yong1, 2, 3; Zhang, Cheng-Cai4; Chen, Yuxing1, 2, 3; Zhou, Cong-Zhao1, 2, 3
关键词: carbon metabolism ; nitrogen metabolism ; transcription factor ; 2-oxoglutarate ; 2-phosphoglycolate
刊名: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
发表日期: 2018-01-09
DOI: 10.1073/pnas.1716062115
卷: 115, 期:2, 页:403-408
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
英文摘要: The coordination of carbon and nitrogen metabolism is essential for bacteria to adapt to nutritional variations in the environment, but the underlying mechanism remains poorly understood. In autotrophic cyanobacteria, high CO2 levels favor the carboxylase activity of ribulose 1,5 bisphosphate carboxylase/oxygenase (RuBisCO) to produce 3-phosphoglycerate, whereas low CO2 levels promote the oxygenase activity of RuBisCO, leading to 2-phosphoglycolate (2-PG) production. Thus, the 2-PG level is reversely correlated with that of 2-oxoglutarate (2-OG), which accumulates under a high carbon/nitrogen ratio and acts as a nitrogen-starvation signal. The LysR-type transcriptional repressor NAD(P)H dehydrogenase regulator (NdhR) controls the expression of genes related to carbon metabolism. Based on genetic and biochemical studies, we report here that 2-PG is an inducer of NdhR, while 2-OG is a corepressor, as found previously. Furthermore, structural analyses indicate that binding of 2-OG at the interface between the two regulatory domains (RD) allows the NdhR tetramer to adopt a repressor conformation, whereas 2-PG binding to an intradomain cleft of each RD triggers drastic conformational changes leading to the dissociation of NdhR from its target DNA. We further confirmed the effect of 2-PG or 2-OG levels on the transcription of the NdhR reg-ulon. Together with previous findings, we propose that NdhR can sense 2-OG from the Krebs cycle and 2-PG from photorespiration, two key metabolites that function together as indicators of intracellular carbon/nitrogen status, thus representing a fine sensor for the coordination of carbon and nitrogen metabolism in cyanobacteria.
关键词[WOS]: INTRACELLULAR 2-OXOGLUTARATE LEVELS ; BICARBONATE TRANSPORTER OPERON ; CO2 CONCENTRATING MECHANISM ; LOW CO2-INDUCED ACTIVATION ; SYNECHOCYSTIS SP PCC-6803 ; SP PCC 6803 ; INORGANIC CARBON ; TRANSCRIPTIONAL REGULATORS ; ESCHERICHIA-COLI ; LYSR FAMILY
语种: 英语
项目资助者: National Natural Science Foundation of China(31630001 ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDB08020300) ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences(QYZDJ-SSW-SMC016) ; Innovation Center for Cell Signaling Network ; 31500598 ; 21573205)
WOS记录号: WOS:000419686400062
ISSN号: 0027-8424
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内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/50627
Appears in Collections:藻类生物学及应用研究中心_期刊论文

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作者单位: 1.Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, Hefei 230027, Anhui, Peoples R China
2.Univ Sci & Technol China, Sch Life Sci, Hefei 230027, Anhui, Peoples R China
3.Univ Sci & Technol China, Key Lab Struct Biol, Hefei 230027, Anhui, Peoples R China
4.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Hubei, Peoples R China
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