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Fam60al as a novel factor involved in reprogramming of somatic cell nuclear transfer in zebrafish (Danio rerio)
Hu, Hongling1,2; Tao, Binbin1,2; Chen, Ji1; Zhu, Zuoyan1; Hu, Wei1,3
2018
Source PublicationINTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
ISSN1449-2288
Volume14Issue:1Pages:78-86
AbstractThe main reason for abnormal development of cloned animals or embryos, and inefficient animal cloning, is a poor understanding of the reprogramming mechanism. To better comprehend reprogramming and subsequent generation of pluripotent stem cells, we must investigate factors related to reprogramming of somatic cells as nuclear donors. As we know, fam60al (family with sequence similarity 60, member A, like) is a coding gene only found in zebrafish and frog (Xenopus laevis) among vertebrates. However, until now, its functions have remained unknown. Here, we generated a zebrafish fam60al(-/-) mutant line using transcription activator-like effector nucleases (TALENs), and found that both nanog and klf4b expression significantly decreased while myca expression significantly increased in fam60al(-/-) mutant embryos. Concurrently, we also uncovered that in developmentally arrested embryos of somatic cell nuclear transfer, nanog, klf4b and myca expression was down-regulated, accompanying a decrease of fam60al expression. Interestingly, we identified a long noncoding RNA (lncRNA) of fam60al, named fam60al-AS, which negatively regulated fam60al by forming double-stranded RNA (dsRNA). RNase protection assay and real-time PCR confirmed these findings. Taken together, these results suggest that fam60al is a novel factor related to the reprogramming of somatic cell nuclear transfer in zebrafish, which is regulated by its reverse lncRNA.
SubtypeArticle
KeywordFam60al Long Noncoding Rna Somatic Cell Nuclear Transfer Reprogramming Nanog Myca
DOI10.7150/ijbs.22426
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationNational Natural Science Foundation(31325026 ; National Natural Science Foundation(31325026 ; Chinese Academy of Sciences(XDA08010106 ; Chinese Academy of Sciences(XDA08010106 ; 31721005) ; 31721005) ; 2016FBZ03) ; 2016FBZ03) ; National Natural Science Foundation(31325026 ; National Natural Science Foundation(31325026 ; Chinese Academy of Sciences(XDA08010106 ; Chinese Academy of Sciences(XDA08010106 ; 31721005) ; 31721005) ; 2016FBZ03) ; 2016FBZ03)
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics
WOS SubjectBiochemistry & Molecular Biology ; Biology
WOS KeywordLONG NONCODING RNAS ; PLURIPOTENT STEM-CELLS ; ANTISENSE RNA ; GENE-EXPRESSION ; DNA-DAMAGE ; ADULT ; NANOG ; TRANSCRIPTS ; SEQUENCE ; CLONING
WOS IDWOS:000422769400008
Funding OrganizationNational Natural Science Foundation(31325026 ; National Natural Science Foundation(31325026 ; Chinese Academy of Sciences(XDA08010106 ; Chinese Academy of Sciences(XDA08010106 ; 31721005) ; 31721005) ; 2016FBZ03) ; 2016FBZ03) ; National Natural Science Foundation(31325026 ; National Natural Science Foundation(31325026 ; Chinese Academy of Sciences(XDA08010106 ; Chinese Academy of Sciences(XDA08010106 ; 31721005) ; 31721005) ; 2016FBZ03) ; 2016FBZ03)
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/50621
Collection鱼类生物学及渔业生物技术研究中心
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Qingdao 266237, Peoples R China
Recommended Citation
GB/T 7714
Hu, Hongling,Tao, Binbin,Chen, Ji,et al. Fam60al as a novel factor involved in reprogramming of somatic cell nuclear transfer in zebrafish (Danio rerio)[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES,2018,14(1):78-86.
APA Hu, Hongling,Tao, Binbin,Chen, Ji,Zhu, Zuoyan,&Hu, Wei.(2018).Fam60al as a novel factor involved in reprogramming of somatic cell nuclear transfer in zebrafish (Danio rerio).INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES,14(1),78-86.
MLA Hu, Hongling,et al."Fam60al as a novel factor involved in reprogramming of somatic cell nuclear transfer in zebrafish (Danio rerio)".INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES 14.1(2018):78-86.
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