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题名: Essential roles of &ITstat5.1&IT/&ITstat5b&IT in controlling fish somatic growth
作者: Xiong, Shuting1; Mei, Jie1; Huang, Peipei2; Jing, Jing1, 3; Li, Zhi2; Kang, Jingliang2; Gui, Jian-Fang1, 2
关键词: Zebrafish ; Stat5 ; Body growth ; Sexual size dimorphism ; Growth hormone
刊名: JOURNAL OF GENETICS AND GENOMICS
发表日期: 2017-12-20
DOI: 10.1016/j.jgg.2017.07.006
卷: 44, 期:12, 页:577-585
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology ; Genetics & Heredity
研究领域[WOS]: Biochemistry & Molecular Biology ; Genetics & Heredity
英文摘要: Signal transducer and activator of transcription 5b (STAT5b) has been identified as a key downstream mediator of growth hormone (GH) signaling in somatic growth of mammalian. However, the corresponding homologue gene of Stat5b is unknown in fish species. In this study, we generated loss-of-function mutants in stat5.1 and stat5.2, two stat5 homologues existing in zebrafish. In stat5.1-deficient zebrafish, a significant reduction of body length and body weight was detected in the embryos/larvae and adults compared with the wild-type control fish, and sexual size dimorphism in adult zebrafish was also eliminated. However, the stat5.2-deficient zebrafish displayed a normal developmental phenotype during all lifespan. Chromatin immunoprecipitation combined with deep sequencing (ChIP-seq) method was adopted to further investigate the potential transcriptional targets of Stat5 protein and cast much light upon the biological function of Stat5. We identified more than 800 genes as transcriptional targets of Stat5 during zebrafish embryogenesis. KEGG analysis indicated that the Stat5 target gene network is predominantly linked to the metabolic pathways, neuroactive ligand-receptor interaction and JAK-STAT signaling pathways. Further validation studies suggested that Stat5.1 protein could directly regulate the expression of gilt and stat5.1-mutated zebrafish showed a reduction of gh1 mRNA level. In the present study, stat5.1 was revealed as the corresponding homologue gene of Stat5b in fish species. Additionally, we found a novel molecular interaction between Stat5.1/Stat5b and GH, and unraveled a positive feedback loop Stat5.1-GH-Stat5.1 which is necessary for somatic growth and body development in zebrafish. Copyright (C) 2017, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and Genetics Society of China. Published by Elsevier Limited and Science Press. All rights reserved.
关键词[WOS]: SEXUAL SIZE DIMORPHISM ; GH/IGF AXIS GENES ; YELLOW CATFISH ; FACTOR-I ; BONE-DEVELOPMENT ; HORMONE ; ZEBRAFISH ; EXPRESSION ; LIVER ; CARP
语种: 英语
项目资助者: Autonomous Projects of the Institute of Hydrobiology, Chinese Academy of Sciences(Y25A17 ; Fundamental Research Funds for the Central Universities(2662017PY013 ; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology(2011FBZ22) ; Y45A171301) ; 2662015PY101)
WOS记录号: WOS:000418884000003
ISSN号: 1673-8527
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内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/50608
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Huazhong Agr Univ, Minist Agr, Key Lab Freshwater Anim Breeding, Coll Fisheries, Wuhan 430070, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
3.Wuhan Univ, Med Res Inst, Wuhan 430071, Hubei, Peoples R China
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