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Nanog suppresses the expression of vasa by directly regulating nlkl in the early zebrafish embryo
Liu, Yanhua1; Xue, Weiwei1; Zhu, Lin2; Ye, Ding2; Zhu, Xiaoqin1; Wang, Huannan1; Sun, Yonghua2; Deng, Fengjiao1
2017-11-01
Source PublicationBIOCHIMIE
ISSN0300-9084
Volume142Pages:93-101
AbstractNanog is a homeodomain transcription factor that is essential for maintenance of pluripotency and self renewal of embryonic stem cells (ESCs). In the present study, we demonstrate that zebrafish Nanog (zNanog) directly binds to the promoter region of zebrafish nIkl (znIkl) by ChIP-Seq analysis and that it up-regulates the expression of znIkl in fibroblast-like embryonic cells of Danio rerio (ZEM-2S cells) and in zebrafish embryos at 30% epiboly both at the mRNA and protein levels. In addition, compared with control (MO-C) embryos at 30% epiboly, the mRNA and protein expression of vasa and the numbers of vasa-positive cells were increased in embryos injected with zNanog morpholino (MO-zNanog). Further, injection of znIk1 mRNA into zNanog-depleted embryos restored the expression of vasa and the number of vasa-positive cells. These data indicated that zNanog up-regulates the expression of znIki through directly binding to the znIkl promoter, thereby suppressing the expression of vasa. Vasa is a marker gene for PGCs. Our results suggest that zNanog plays a role in restraint of PGC cell number through regulating the expression of znIkl in the early embryonic development. The current results provide fundamental information to support further investigation regarding the regulatory mechanism of zNanog during the development of PGCs. (C) 2017 Published by Elsevier B.V.
SubtypeArticle
KeywordZnanog Znikl Pgcs Zebrafish Embryonic Development
DOI10.1016/j.biochi.2017.07.014
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationNational Natural Science Foundation of China(31372147) ; National Natural Science Foundation of China(31372147) ; National Natural Science Foundation of China(31372147) ; National Natural Science Foundation of China(31372147)
Language英语
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemistry & Molecular Biology
WOS KeywordPRIMORDIAL GERM-CELLS ; STEM-CELLS ; MIGRATION ; DIFFERENTIATION ; PLURIPOTENCY ; PERSPECTIVE ; ACTIVATION ; KNOCKDOWN ; EPIBLAST ; HOMOLOG
WOS IDWOS:000415780500009
Funding OrganizationNational Natural Science Foundation of China(31372147) ; National Natural Science Foundation of China(31372147) ; National Natural Science Foundation of China(31372147) ; National Natural Science Foundation of China(31372147)
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/50594
Collection鱼类生物学及渔业生物技术研究中心
Affiliation1.Wuhan Univ, Coll Life Sci, Lab Mol Genet & Dev Biol, Wuhan 430072, Hubei, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Liu, Yanhua,Xue, Weiwei,Zhu, Lin,et al. Nanog suppresses the expression of vasa by directly regulating nlkl in the early zebrafish embryo[J]. BIOCHIMIE,2017,142:93-101.
APA Liu, Yanhua.,Xue, Weiwei.,Zhu, Lin.,Ye, Ding.,Zhu, Xiaoqin.,...&Deng, Fengjiao.(2017).Nanog suppresses the expression of vasa by directly regulating nlkl in the early zebrafish embryo.BIOCHIMIE,142,93-101.
MLA Liu, Yanhua,et al."Nanog suppresses the expression of vasa by directly regulating nlkl in the early zebrafish embryo".BIOCHIMIE 142(2017):93-101.
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