IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心
Involvement of two glycoside hydrolase family 19 members in colony morphotype and virulence in Flavobacterium columnare
Zhang Xiaolin1,2; Li Nan1; Qin Ting1; Huang Bei3; Nie Pin1
2017-11-01
Source PublicationCHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN0254-4059
Volume35Issue:6Pages:1511-1523
AbstractFlavobacterium columnare is the pathogenic agent of columnaris disease in aquaculture. Using a recently developed gene deletion strategy, two genes that encode the Glyco_hydro_19 domain (GH19 domain) containing proteins, ghd-1 and ghd-2, were deleted separately and together from the F. columnare G(4) wild type strain. Surprisingly, the single-, Delta ghd-1 and Delta ghd-2, and double-gene mutants, Delta ghd-1 Delta ghd-2, all had rhizoid and non-rhizoid colony morphotypes, which we named Delta ghd-1, Delta ghd-2, Delta ghd-1 Delta ghd-2, and N Delta ghd-1, N Delta ghd-2, and N Delta ghd-1 Delta ghd-2. However, chitin utilization was not detected in either these mutants or in the wild type. Instead, skimmed milk degradation was observed for the mutants and the wild type; the non-rhizoid strain N Delta ghd-2 exhibited higher degradation activity as revealed by the larger transparent circle on the skimmed milk plate. Using zebrafish as the model organism, we found that non-rhizoid mutants had higher LD50 values and were less virulent because zebrafish infected with these survived longer. Transcriptome analysis between the non-rhizoid and rhizoid colony morphotypes of each mutant, i.e., N Delta ghd -1 versus (vs) Delta ghd-1, N Delta ghd-2 vs Delta ghd-2, and N Delta ghd-1 Delta ghd-2 vs Delta ghd-1 Delta ghd-2, revealed a large number of differentially expressed genes, among which 39 genes were common in three of the pairs compared. Although most of these genes encode hypothetical proteins, a few molecules such as phage tail protein, rhs element Vgr protein, thiol-activated cytolysin, and TonB-dependent outer membrane receptor precursor, expression of which was down-regulated in non-rhizoid mutants but up-regulated in rhizoid mutants, may play a role F. columnare virulence.
SubtypeArticle
KeywordFlavobacterium Columnare Gh19 Domain Gene Deletion Rhizoid Colony Non-rhizoid Colony
DOI10.1007/s00343-017-6160-z
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
Indexed BySCI
Funding OrganizationChinese Academy of Sciences(XDA08010207) ; Chinese Academy of Sciences(XDA08010207) ; National Key Technology R& D Program of China(2012BAD25B02) ; National Key Technology R& D Program of China(2012BAD25B02) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2016FBZ04) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2016FBZ04) ; Chinese Academy of Sciences(XDA08010207) ; Chinese Academy of Sciences(XDA08010207) ; National Key Technology R& D Program of China(2012BAD25B02) ; National Key Technology R& D Program of China(2012BAD25B02) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2016FBZ04) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2016FBZ04)
Language英语
WOS Research AreaMarine & Freshwater Biology ; Oceanography
WOS SubjectLimnology ; Oceanography
WOS KeywordCHONDROITIN AC LYASE ; IX SECRETION SYSTEM ; FISH PATHOGEN ; GLIDING MOTILITY ; FLEXIBACTER-COLUMNARIS ; GENOME SEQUENCE ; CHITIN ; ADHESION ; GENE ; PSYCHROPHILUM
WOS IDWOS:000415810900022
Funding OrganizationChinese Academy of Sciences(XDA08010207) ; Chinese Academy of Sciences(XDA08010207) ; National Key Technology R& D Program of China(2012BAD25B02) ; National Key Technology R& D Program of China(2012BAD25B02) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2016FBZ04) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2016FBZ04) ; Chinese Academy of Sciences(XDA08010207) ; Chinese Academy of Sciences(XDA08010207) ; National Key Technology R& D Program of China(2012BAD25B02) ; National Key Technology R& D Program of China(2012BAD25B02) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2016FBZ04) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2016FBZ04)
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/50592
Collection鱼类生物学及渔业生物技术研究中心
Affiliation1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Jimei Univ, Coll Fisheries, Xiamen 361021, Peoples R China
Recommended Citation
GB/T 7714
Zhang Xiaolin,Li Nan,Qin Ting,et al. Involvement of two glycoside hydrolase family 19 members in colony morphotype and virulence in Flavobacterium columnare[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2017,35(6):1511-1523.
APA Zhang Xiaolin,Li Nan,Qin Ting,Huang Bei,&Nie Pin.(2017).Involvement of two glycoside hydrolase family 19 members in colony morphotype and virulence in Flavobacterium columnare.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,35(6),1511-1523.
MLA Zhang Xiaolin,et al."Involvement of two glycoside hydrolase family 19 members in colony morphotype and virulence in Flavobacterium columnare".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 35.6(2017):1511-1523.
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