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IHB OpenIR  > 中科院水生所知识产出(2009年前)  > 期刊论文
题名: GENE-TRANSFER VIA ELECTROPORATION IN FISH
作者: XIE, YF; LIU, D; ZOU, J; LI, GH; ZHU, ZY
刊名: AQUACULTURE
发表日期: 1993-04-01
卷: 111, 期:1-4, 页:207-213
收录类别: ISTP ; SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Fisheries ; Marine & Freshwater Biology
研究领域[WOS]: Fisheries ; Marine & Freshwater Biology
英文摘要: A fusion human growth hormone gene driven by the mouse metallothionein-1 promoter was successfully introduced into the fertilized eggs of loach and red crucian carp using a capacitor-based electroporator. The results of DNA hybridization showed that the transfer efficiency and the transgene copy number per genome were positively correlated to the voltage, duration and capacity of the pulse. Under optimum conditions, a transfer efficiency of up to 62.5% was achieved. Some fish arising from embryos treated in this manner exhibited over 100 copies of the transgene integrated into their genomes.
关键词[WOS]: TRANSGENIC FISH ; EGGS
语种: 英语
WOS记录号: WOS:A1993KZ99900022
ISSN号: 0044-8486
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/50578
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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