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GENE-TRANSFER VIA ELECTROPORATION IN FISH
XIE, YF; LIU, D; ZOU, J; LI, GH; ZHU, ZY
1993-04-01
Source PublicationAQUACULTURE
ISSN0044-8486
Volume111Issue:1-4Pages:207-213
AbstractA fusion human growth hormone gene driven by the mouse metallothionein-1 promoter was successfully introduced into the fertilized eggs of loach and red crucian carp using a capacitor-based electroporator. The results of DNA hybridization showed that the transfer efficiency and the transgene copy number per genome were positively correlated to the voltage, duration and capacity of the pulse. Under optimum conditions, a transfer efficiency of up to 62.5% was achieved. Some fish arising from embryos treated in this manner exhibited over 100 copies of the transgene integrated into their genomes.
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed ByISTP ; SCI
Language英语
WOS Research AreaFisheries ; Marine & Freshwater Biology
WOS SubjectFisheries ; Marine & Freshwater Biology
WOS KeywordTRANSGENIC FISH ; EGGS
WOS IDWOS:A1993KZ99900022
Citation statistics
Cited Times:17[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/50578
Collection中科院水生所知识产出(2009年前)
Recommended Citation
GB/T 7714
XIE, YF,LIU, D,ZOU, J,et al. GENE-TRANSFER VIA ELECTROPORATION IN FISH[J]. AQUACULTURE,1993,111(1-4):207-213.
APA XIE, YF,LIU, D,ZOU, J,LI, GH,&ZHU, ZY.(1993).GENE-TRANSFER VIA ELECTROPORATION IN FISH.AQUACULTURE,111(1-4),207-213.
MLA XIE, YF,et al."GENE-TRANSFER VIA ELECTROPORATION IN FISH".AQUACULTURE 111.1-4(1993):207-213.
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