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题名: Integrated Proteomic and Transcriptomic Analysis Reveals Long Noncoding RNA HOX Transcript Antisense Intergenic RNA (HOTAIR) Promotes Hepatocellular Carcinoma Cell Proliferation by Regulating Opioid Growth Factor Receptor (OGFr)
作者: Wu, Ying1, 2; Xiong, Qian1; Li, Siting1, 2; Yang, Xue1, 2; Ge, Feng1
刊名: MOLECULAR & CELLULAR PROTEOMICS
发表日期: 2018
DOI: 10.1074/mcp.RA117.000277
卷: 17, 期:1, 页:18-31
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Biochemical Research Methods
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: Long noncoding RNA HOX transcript antisense RNA (HOTAIR) is involved in human tumorigenesis and is dysregulated in hepatocellular carcinoma (HCC). However, the molecular mechanisms underlying HOTAIR functions in HCC are largely unknown. Here, we employed an integrated transcriptomic and quantitative proteomic analysis to systematically explore the regulatory role of HOTAIR in HCC. A total of 673 transcripts and 293 proteins were found to be dysregulated after HOTAIR inhibition. Bioinformatics studies indicated that differentially expressed genes (DEGs) and differentially expressed proteins (DEPs) are involved in many biological processes, especially cancer-related signaling pathways. A set of DEGs and DEPs were validated by quantitative RT-PCR, Western blot and parallel reaction monitoring (PRM) analysis, respectively. Further functional studies of the opioid growth factor receptor (OGFr), a negative biological regulator of cell proliferation in HCC, revealed that HOTAIR exerts its effects on cell proliferation, at least in part, through the regulation of OGFr expression. By correlating the omics data with functional studies, the current results provide novel insights into the functional mechanisms of HOTAIR in HCC cells.
关键词[WOS]: LARGE GENE LISTS ; GASTRIC-CANCER ; OVARIAN-CANCER ; INTERACTION NETWORKS ; CERVICAL-CANCER ; POOR-PROGNOSIS ; UP-REGULATION ; EXPRESSION ; AXIS ; PROGRESSION
语种: 英语
项目资助者: National Natural Science Foundation of China(31370746 ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDB14030202) ; 91540102)
WOS记录号: WOS:000419169700003
ISSN号: 1535-9476
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内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/43677
Appears in Collections:水生生物分子与细胞生物学研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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