Claudin7b is required for the formation and function of inner ear in zebrafish
Li, Xiaohui1,2; Song, Guili1; Zhao, Yasong1,2; Zhao, Feng3; Liu, Chunyan1; Liu, Dong3; Li, Qing1; Cui, Zongbin1
2018-04-01
Source PublicationJOURNAL OF CELLULAR PHYSIOLOGY
ISSN0021-9541
Volume233Issue:4Pages:3195-3206
AbstractZebrafish has become an excellent model for studying the development and function of inner ear. We report here a zebrafish line in which claudin 7b (cldn7b) locus is interrupted by a Tol2 transposon at its first intron. The homozygous mutants have enlarged otocysts, smaller or no otoliths, slowly formed semicircular canals, and insensitiveness to sound stimulation. These abnormal phenotypes and hearing loss of inner ear could be mostly rescued by injection of cldn7b-mRNA into one-cell stage homozygous mutant embryos. Mechanistically, cldn7b-deficiency interrupted the formation of apical junction complexes (AJCs) in otic epithelial cells of inner ear and the ion-homeostasis of endolymph, which then led to the loss of proper contact between otoliths and normally developed hair cells in utricle and saccule or aberrant mechanosensory transduction. Thus, Cldn7b is essential for the formation and proper function of inner ear through its unique role in keeping an initial integrity of otic epithelia during zebrafish embryogenesis.
SubtypeArticle
KeywordClaudin7b Hearing Loss Inner Ear Tight Junction Zebrafish
DOI10.1002/jcp.26162
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationNational Natural Science Foundation of China(31571504 ; National Natural Science Foundation of China(31571504 ; National Basic Research Program of China(2012CB944500) ; National Basic Research Program of China(2012CB944500) ; 31571496) ; 31571496) ; National Natural Science Foundation of China(31571504 ; National Natural Science Foundation of China(31571504 ; National Basic Research Program of China(2012CB944500) ; National Basic Research Program of China(2012CB944500) ; 31571496) ; 31571496)
Language英语
WOS Research AreaCell Biology ; Physiology
WOS SubjectCell Biology ; Physiology
WOS KeywordOTOLITH FORMATION ; CILIARY MOTILITY ; TIGHT JUNCTION ; VERTEBRATE EAR ; HAIR-CELLS ; EXPRESSION ; EMBRYOS ; MECHANISMS ; COMPLEX ; ABILITY
WOS IDWOS:000418723200050
Funding OrganizationNational Natural Science Foundation of China(31571504 ; National Natural Science Foundation of China(31571504 ; National Basic Research Program of China(2012CB944500) ; National Basic Research Program of China(2012CB944500) ; 31571496) ; 31571496) ; National Natural Science Foundation of China(31571504 ; National Natural Science Foundation of China(31571504 ; National Basic Research Program of China(2012CB944500) ; National Basic Research Program of China(2012CB944500) ; 31571496) ; 31571496)
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/43673
Collection藻类生物学及应用研究中心_水生生物分子与细胞生物学研究中心
Affiliation1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Southern Univ Sci & Technol, Dept Biol, Shenzhen, Peoples R China
Recommended Citation
GB/T 7714
Li, Xiaohui,Song, Guili,Zhao, Yasong,et al. Claudin7b is required for the formation and function of inner ear in zebrafish[J]. JOURNAL OF CELLULAR PHYSIOLOGY,2018,233(4):3195-3206.
APA Li, Xiaohui.,Song, Guili.,Zhao, Yasong.,Zhao, Feng.,Liu, Chunyan.,...&Cui, Zongbin.(2018).Claudin7b is required for the formation and function of inner ear in zebrafish.JOURNAL OF CELLULAR PHYSIOLOGY,233(4),3195-3206.
MLA Li, Xiaohui,et al."Claudin7b is required for the formation and function of inner ear in zebrafish".JOURNAL OF CELLULAR PHYSIOLOGY 233.4(2018):3195-3206.
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