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题名: Raman-Activated Droplet Sorting (RADS) for Label-Free High-Throughput Screening of Microalgal Single-Cells
作者: Wang, Xixian1, 2, 4; Ren, Lihui1, 2, 4; Su, Yetian1, 2; Ji, Yuetong1, 2, 4; Liu, Yaoping5; Li, Chunyu1, 2, 4; Li, Xunrong1, 2, 4; Zhang, Yi1, 2; Wang, Wei5; Hu, Qiang3; Han, Danxiang3; Xu, Jian1, 2, 4; Ma, Bo1, 2, 4
刊名: ANALYTICAL CHEMISTRY
发表日期: 2017-11-21
DOI: 10.1021/acs.analchem.7b03884
卷: 89, 期:22, 页:12569-12577
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Raman-activated cell sorting (RACS) has attracted increasing interest, yet throughput remains one major factor limiting its broader application. Here we present an integrated Raman-activated droplet sorting (RADS) microfluidic system for functional screening of live cells in a label-free and high-throughput manner, by employing AXT-synthetic industrial microalga Haematococcus pluvialis (H. pluvialis) as a model. Raman microspectroscopy analysis of individual cells is carried out prior to their microdroplet encapsulation, which is then directly coupled to DEP-based droplet sorting. To validate the system, H. pluvialis cells containing different levels of AXT were mixed and underwent RADS. Those AXT-hyperproducing cells were sorted with an accuracy of 98.3%, an enrichment ratio of eight folds, and a throughput of similar to 260 cells/min. Of the RADS-sorted cells, 92.7% remained alive and able to proliferate, which is equivalent to the unsorted cells. Thus, the RADS achieves a much higher throughput than existing RACS systems, preserves the vitality of cells, and facilitates seamless coupling with downstream manipulations such as single-cell sequencing and cultivation.
关键词[WOS]: HAEMATOCOCCUS-PLUVIALIS MUTANTS ; MICROFLUIDIC FLOW ; MICROBIAL-CELLS ; STRESS-RESPONSE ; GREEN-ALGA ; SPECTROSCOPY ; ASTAXANTHIN ; EVOLUTION ; TWEEZERS ; IDENTIFICATION
语种: 英语
项目资助者: China Postdoctoral Science Foundation ; National Natural Science Foundation of China(31600076 ; Ministry of Science and Technology of China(2016YFF0202303) ; Chinese Academy of Sciences(KFJ-SW-STS-165 ; China Postdoctoral Science Foundation(2017M612364 ; Shandong Province Natural Science Foundation(ZR2014CQ005) ; State Development & Investment Corporation (SDIC) ; 31470220 ; XDBP0305) ; 2017T100522) ; 31670101 ; 31327001 ; 31601084 ; 21775155 ; 31425002)
WOS记录号: WOS:000416498100092
ISSN号: 0003-2700
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/43581
Appears in Collections:藻类生物学及应用研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Single Cell Ctr, CAS Key Lab Biofuels, Qingdao 266101, Shandong, Peoples R China
2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Key Lab Energy Genet, Qingdao 266101, Shandong, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, Ctr Microalgal Biofuels & Biotechnol, Wuhan 430072, Hubei, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
5.Peking Univ, Inst Microelect, Natl Key Lab Sci & Technol Micro Nano Fabricat, Beijing 100871, Peoples R China
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