IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Cultivation and Characterization of the MaMV-DC Cyanophage that Infects Bloom-forming Cyanobacterium Microcystis aeruginosa
Ou Tong; Li Sanhua; Liao Xiangyong; Zhang Qiya
2013
Source Publication中国病毒学:英文版
ISSN1674-0769
Volume000Issue:005Pages:266
AbstractThe MaMV-DC cyanophage, which infects the bloom-forming cyanobacterium Microcystis aeruginosa, was isolated from Lake Dianchi, Kunming, China. Twenty-one cyanobacterial strains were used to detect the host range of MaMV-DC. Microcystic aeruginosa FACHB-524 and plaque purification were used to isolate individual cyanophages, and culturing MaMV-DC with cyanobacteria allowed us to prepare purified cyanophages for further analysis. Electron microscopy demonstrated that the negatively stained viral particles are tadpole-shaped with an icosahedral head approximately 70 nm in diameter and a contractile tail approximately 160 nm in length. Using one-step growth experiments, the latent period and burst size of MaMV-DC were estimated to be 2448 hours and approximately 80 infectious units per cell, respectively. Restriction endonuclease digestion and agarose gel electrophoresis were performed using purified MaMV-DC genomic DNA, and the genome size was estimated to be approximately 160 kb. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis revealed four major structural proteins. These results support the growing interest in using freshwater cyanophages to control bloom-forming cyanobacterium.
Keyword铜绿微囊藻 水华蓝藻 噬藻体 感染 聚丙烯酰胺凝胶电泳 SDS-PAGE 基因组DNA 琼脂糖凝胶电泳
Language英语
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/42634
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Affiliation中国科学院水生生物研究所
Recommended Citation
GB/T 7714
Ou Tong,Li Sanhua,Liao Xiangyong,et al. Cultivation and Characterization of the MaMV-DC Cyanophage that Infects Bloom-forming Cyanobacterium Microcystis aeruginosa[J]. 中国病毒学:英文版,2013,000(005):266.
APA Ou Tong,Li Sanhua,Liao Xiangyong,&Zhang Qiya.(2013).Cultivation and Characterization of the MaMV-DC Cyanophage that Infects Bloom-forming Cyanobacterium Microcystis aeruginosa.中国病毒学:英文版,000(005),266.
MLA Ou Tong,et al."Cultivation and Characterization of the MaMV-DC Cyanophage that Infects Bloom-forming Cyanobacterium Microcystis aeruginosa".中国病毒学:英文版 000.005(2013):266.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Ou Tong]'s Articles
[Li Sanhua]'s Articles
[Liao Xiangyong]'s Articles
Baidu academic
Similar articles in Baidu academic
[Ou Tong]'s Articles
[Li Sanhua]'s Articles
[Liao Xiangyong]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Ou Tong]'s Articles
[Li Sanhua]'s Articles
[Liao Xiangyong]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.