| Cultivation and Characterization of the MaMV-DC Cyanophage that Infects Bloom-forming Cyanobacterium Microcystis aeruginosa | |
| Ou Tong; Li Sanhua; Liao Xiangyong; Zhang Qiya | |
| 2013 | |
| Source Publication | 中国病毒学：英文版
 |
| ISSN | 1674-0769 |
| Volume | 000Issue:005Pages:266 |
| Abstract | The MaMV-DC cyanophage, which infects the bloom-forming cyanobacterium Microcystis aeruginosa, was isolated from Lake Dianchi, Kunming, China. Twenty-one cyanobacterial strains were used to detect the host range of MaMV-DC. Microcystic aeruginosa FACHB-524 and plaque purification were used to isolate individual cyanophages, and culturing MaMV-DC with cyanobacteria allowed us to prepare purified cyanophages for further analysis. Electron microscopy demonstrated that the negatively stained viral particles are tadpole-shaped with an icosahedral head approximately 70 nm in diameter and a contractile tail approximately 160 nm in length. Using one-step growth experiments, the latent period and burst size of MaMV-DC were estimated to be 2448 hours and approximately 80 infectious units per cell, respectively. Restriction endonuclease digestion and agarose gel electrophoresis were performed using purified MaMV-DC genomic DNA, and the genome size was estimated to be approximately 160 kb. Sodium dodecyl sulfate polyacrylamide gel electrophoresis （SDS-PAGE） analysis revealed four major structural proteins. These results support the growing interest in using freshwater cyanophages to control bloom-forming cyanobacterium. |
| Keyword | 铜绿微囊藻 水华蓝藻 噬藻体 感染 聚丙烯酰胺凝胶电泳 SDS-PAGE 基因组DNA 琼脂糖凝胶电泳 |
| Language | 英语 |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/42634 |
| Collection | 鱼类生物学及渔业生物技术研究中心_期刊论文 |
| Affiliation | 中国科学院水生生物研究所 |
| Recommended Citation GB/T 7714 | Ou Tong,Li Sanhua,Liao Xiangyong,et al. Cultivation and Characterization of the MaMV-DC Cyanophage that Infects Bloom-forming Cyanobacterium Microcystis aeruginosa[J]. 中国病毒学：英文版,2013,000(005):266. |
| APA | Ou Tong,Li Sanhua,Liao Xiangyong,&Zhang Qiya.(2013).Cultivation and Characterization of the MaMV-DC Cyanophage that Infects Bloom-forming Cyanobacterium Microcystis aeruginosa.中国病毒学：英文版,000(005),266. |
| MLA | Ou Tong,et al."Cultivation and Characterization of the MaMV-DC Cyanophage that Infects Bloom-forming Cyanobacterium Microcystis aeruginosa".中国病毒学：英文版 000.005(2013):266. |
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